Strain Name:
C57BL/6J-MtgxR5473Btlr/Mmmh
Stock Number:
043034-MU
Citation ID:
RRID:MMRRC_043034-MU
Other Names:
R5473 (G1), C57BL/6J-MtgxR5473Btlr
Major Collection:

Gene Information

Col2a1
Name: collagen, type II, alpha 1
Synonyms: Del1, Lpk, M100856, Col2, Rgsc856, Col2a, Col2a-1, M100413, Rgsc413
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 12824
HGNC: HGNC:2200
Homologene: 55607
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 18193
VEGA: 13
Homologene: 32543
Birc5
Name: baculoviral IAP repeat-containing 5
Synonyms: TIAP, survivin, Api4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11799
HGNC: HGNC:593
Homologene: 37450
Rsrc2
Name: arginine/serine-rich coiled-coil 2
Synonyms: 1500011J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 208606
Homologene: 133892
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Mcpr, Apc1, 2610021O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17222
Homologene: 7414
Mdc1
Name: mediator of DNA damage checkpoint 1
Synonyms: NFBD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240087
Homologene: 67092
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: Ayu17-449, E130014J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 214133
Homologene: 49498
Plcg2
Name: phospholipase C, gamma 2
Synonyms: PLCgamma2, Plcg-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234779
HGNC: HGNC:9066
Homologene: 55671
Brca1
Name: breast cancer 1, early onset
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 12189
HGNC: HGNC:1100
Homologene: 5276
Cntrob
Name: centrobin, centrosomal BRCA2 interacting protein
Synonyms: 9830165K03Rik, Nip2, Lip8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216846
Homologene: 14205
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: 4930400K19Rik, 2310003F20Rik, Garnl1, Tulip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 56784
VEGA: 12
Homologene: 84805
Fgd6
Name: FYVE, RhoGEF and PH domain containing 6
Synonyms: ZFYVE24, Etohd4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 13998
Homologene: 14209
Nuf2
Name: NUF2, NDC80 kinetochore complex component
Synonyms: Nuf2R, Cdca1, 2410003C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66977
Homologene: 40205
Mcm3ap
Name: minichromosome maintenance complex component 3 associated protein
Synonyms: GANP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 54387
VEGA: 10
HGNC: HGNC:6946
Homologene: 2902
Prpf31
Name: pre-mRNA processing factor 31
Synonyms: 1500019O16Rik, 2810404O06Rik, RP11, PRP31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 68988
Homologene: 5980
Neo1
Name: neogenin
Synonyms: Igdcc2, 2610028H22Rik, D930014N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 18007
VEGA: 9
HGNC: HGNC:7754
Homologene: 1870
Hnrnpk
Name: heterogeneous nuclear ribonucleoprotein K
Synonyms: Hnrpk, KBBP, hnRNPK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 15387
HGNC: HGNC:5044
Homologene: 81909
Ppfia1
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Synonyms: liprin, C030014K08Rik, Liprin-alpha1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233977
HGNC: HGNC:9245
Homologene: 20802
Pms2
Name: PMS1 homolog2, mismatch repair system component
Synonyms: mismatch repair, DNA mismatch repair
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18861
HGNC: HGNC:9122
Homologene: 133560
Ilf2
Name: interleukin enhancer binding factor 2
Synonyms: TEG-267, Tex261, 6230405A16Rik, Tex267
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 67781
HGNC: HGNC:6037
Homologene: 26894
Igf2r
Name: insulin-like growth factor 2 receptor
Synonyms: CI-MPR, M6P/IGF2R, Mpr300, mannose-6-phosphate receptor, cation independent, CD222, IGF-II/CI-MPR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 16004
HGNC: HGNC:5467
Homologene: 676
Pum3
Name: pumilio RNA-binding family member 3
Synonyms: 1110069H02Rik, D19Bwg1357e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 52874
VEGA: 19
Homologene: 5762
Ccdc47
Name: coiled-coil domain containing 47
Synonyms: asp4, 2610204L23Rik, calumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67163
Homologene: 41351
Rpf2
Name: ribosome production factor 2 homolog
Synonyms: 2810470K21Rik, Bxdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67239
Homologene: 6404
Ankrd26
Name: ankyrin repeat domain 26
Synonyms: 5730521P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232339
Homologene: 45968
Zdhhc5
Name: zinc finger, DHHC domain containing 5
Synonyms: Zisp, 1110032A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228136
Homologene: 9147
Gorasp2
Name: golgi reassembly stacking protein 2
Synonyms: GRS2, 5730520M13Rik, 9430094F20Rik, ENSMUSG00000075299, p59, GRASP55, GOLPH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70231
Homologene: 9180
Wdr92
Name: WD repeat domain 92
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103784
Homologene: 16321
Pcgf3
Name: polycomb group ring finger 3
Synonyms: 2310035N15Rik, D630042K08Rik, RNF3A, Rnf3, DONG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69587
Homologene: 4605
Cpne5
Name: copine V
Synonyms: A830083G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240058
HGNC: HGNC:2318
Homologene: 23266
Col24a1
Name: collagen, type XXIV, alpha 1
Synonyms: 5430404K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 71355
Homologene: 65061
Myl3
Name: myosin, light polypeptide 3
Synonyms: slow skeletal, MLC1v, ventricular, alkali, Mylc, MLC1s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17897
HGNC: HGNC:7584
Homologene: 20099
Camk2d
Name: calcium/calmodulin-dependent protein kinase II, delta
Synonyms: 8030469K03Rik, 2810011D23Rik, CaMK II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 108058
HGNC: HGNC:1462
Homologene: 55561
Madd
Name: MAP-kinase activating death domain
Synonyms: Rab3 GEP, 9630059K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228355
HGNC: HGNC:6766
Homologene: 14249
Scara5
Name: scavenger receptor class A, member 5
Synonyms: 4933425F03Rik, 4932433F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71145
Homologene: 12556
Tdrd7
Name: tudor domain containing 7
Synonyms: 5730495N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100121
Homologene: 8618
Pde1a
Name: phosphodiesterase 1A, calmodulin-dependent
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18573
HGNC: HGNC:8774
Homologene: 21043
Adamtsl4
Name: ADAMTS-like 4
Synonyms: Tsrc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229595
Homologene: 23141
Fam83b
Name: family with sequence similarity 83, member B
Synonyms: C530008M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208994
Homologene: 19478
Nrxn1
Name: neurexin I
Synonyms: neurexin I beta, neurexin I beta, neurexin I alpha, neurexin I alpha, A230068P09Rik, 1700062G21Rik, neurexin I alpha, neurexin I beta, alpha-latrotoxin receptor (calcium-dependent), 9330127H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 18189
HGNC: HGNC:8008
Homologene: 21005
Crat
Name: carnitine acetyltransferase
Synonyms: CARAT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12908
HGNC: HGNC:2342
Homologene: 598
Plagl2
Name: pleiomorphic adenoma gene-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 54711
HGNC: HGNC:9047
Homologene: 1994
Pon3
Name: paraoxonase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 269823
HGNC: HGNC:9206
Homologene: 37371
Olfr218
Name: olfactory receptor 218
Synonyms: Olfr1405-ps1, GA_x6K02T2R7CC-643715-642847, MOR267-3, MOR267-3, GA_x6K02SYWG4P-534-1100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 258880
Homologene: 105155
Hrh4
Name: histamine receptor H4
Synonyms: H4R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225192
VEGA: 18
Homologene: 11002
Kiz
Name: kizuna centrosomal protein
Synonyms: Gm114, Ncrna00153, Plk1s1, LOC228730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228730
Homologene: 10219
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 636808
Homologene: 43974
Ufsp2
Name: UFM1-specific peptidase 2
Synonyms: 1810047C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 192169
Homologene: 10151
Slc30a1
Name: solute carrier family 30 (zinc transporter), member 1
Synonyms: C130040I11Rik, Znt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22782
Homologene: 6681
Eya4
Name: EYA transcriptional coactivator and phosphatase 4
Synonyms: B130023L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 14051
VEGA: 10
HGNC: HGNC:3522
Homologene: 3025
Dlgap1
Name: DLG associated protein 1
Synonyms: SAPAP1, Sapap1, D17Bwg0511e, Gkap, 4933422O14Rik, GKAP/SAPAP, DAP-1 beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 224997
HGNC: HGNC:2905
Homologene: 31258
Pramef8
Name: PRAME family member 8
Synonyms: 4732496O08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242736
Homologene: 128393
Kcnq5
Name: potassium voltage-gated channel, subfamily Q, member 5
Synonyms: 9230107O05Rik, D1Mgi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226922
HGNC: HGNC:6299
Homologene: 28270
Oxsm
Name: 3-oxoacyl-ACP synthase, mitochondrial
Synonyms: 4933425A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 71147
Homologene: 5820
H2-M10.3
Name: histocompatibility 2, M region locus 10.3
Synonyms: 5.3H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 110696
Homologene: 117973
Olfr1166
Name: olfactory receptor 1166
Synonyms: MOR174-6, GA_x6K02T2Q125-49616865-49615915
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258644
Homologene: 138307
Acsm4
Name: acyl-CoA synthetase medium-chain family member 4
Synonyms: OMACS, O-MACS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233801
Homologene: 72281
Ugt1a7c
Name: UDP glucuronosyltransferase 1 family, polypeptide A7C
Synonyms: A10'
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 394432
Homologene: 136043
Saraf
Name: store-operated calcium entry-associated regulatory factor
Synonyms: 1810045K07Rik, Tmem66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67887
Homologene: 9398
Olfr125
Name: olfactory receptor 125
Synonyms: MOR218-8, GA_x6K02T2PSCP-2291580-2292542
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258287
Homologene: 114633
Nlrp5-ps
Name: NLR family, pyrin domain containing 5, pseudogene
Synonyms: Gm18756
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 100417675
Olfr908
Name: olfactory receptor 908
Synonyms: GA_x6K02T2PVTD-32216179-32217111, MOR165-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258872
VEGA: 9
Homologene: 74149
Gm26883
Name: predicted gene, 26883
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 21,457,402 bp
  • T to A, chromosome 1 at 88,095,437 bp
  • T to A, chromosome 1 at 116,089,256 bp
  • T to C, chromosome 1 at 169,507,287 bp
  • G to C, chromosome 1 at 173,204,165 bp
  • T to C, chromosome 1 at 191,909,622 bp
  • A to G, chromosome 2 at 30,407,714 bp
  • A to C, chromosome 2 at 70,678,606 bp
  • G to T, chromosome 2 at 79,906,028 bp
  • A to G, chromosome 2 at 84,690,466 bp
  • A to T, chromosome 2 at 88,124,637 bp
  • A to G, chromosome 2 at 91,170,643 bp
  • G to T, chromosome 2 at 128,607,195 bp
  • G to T, chromosome 2 at 146,969,995 bp
  • A to T, chromosome 2 at 153,232,194 bp
  • T to C, chromosome 2 at 169,883,798 bp
  • C to T, chromosome 3 at 90,485,754 bp
  • G to T, chromosome 3 at 95,679,993 bp
  • G to T, chromosome 3 at 126,597,399 bp
  • G to T, chromosome 3 at 133,466,707 bp
  • A to C, chromosome 3 at 145,537,261 bp
  • G to T, chromosome 4 at 46,020,877 bp
  • A to G, chromosome 4 at 143,419,304 bp
  • C to T, chromosome 5 at 108,473,875 bp
  • C to T, chromosome 5 at 123,731,087 bp
  • T to C, chromosome 5 at 143,916,394 bp
  • A to T, chromosome 6 at 5,256,177 bp
  • A to T, chromosome 6 at 118,515,836 bp
  • A to G, chromosome 7 at 3,639,825 bp
  • G to A, chromosome 7 at 14,580,975 bp
  • T to C, chromosome 7 at 119,712,950 bp
  • A to T, chromosome 7 at 144,491,492 bp
  • G to A, chromosome 8 at 34,161,258 bp
  • A to G, chromosome 8 at 45,992,221 bp
  • A to G, chromosome 8 at 117,634,401 bp
  • A to G, chromosome 9 at 38,516,212 bp
  • T to C, chromosome 9 at 58,880,843 bp
  • T to C, chromosome 9 at 76,491,500 bp
  • C to A, chromosome 9 at 110,767,958 bp
  • T to A, chromosome 10 at 23,163,453 bp
  • A to G, chromosome 10 at 40,227,631 bp
  • T to G, chromosome 10 at 76,502,759 bp
  • T to C, chromosome 10 at 94,044,676 bp
  • T to C, chromosome 11 at 17,224,591 bp
  • G to T, chromosome 11 at 69,322,753 bp
  • G to A, chromosome 11 at 101,512,737 bp
  • A to T, chromosome 11 at 106,205,029 bp
  • T to C, chromosome 11 at 117,852,707 bp
  • T to C, chromosome 12 at 55,676,710 bp
  • A to G, chromosome 13 at 55,247,772 bp
  • A to T, chromosome 13 at 58,394,099 bp
  • A to T, chromosome 14 at 16,242,045 bp
  • T to A, chromosome 14 at 65,740,339 bp
  • G to T, chromosome 15 at 97,987,489 bp
  • G to T, chromosome 17 at 12,695,314 bp
  • G to A, chromosome 17 at 29,160,365 bp
  • A to G, chromosome 17 at 35,848,060 bp
  • A to T, chromosome 17 at 36,367,369 bp
  • T to C, chromosome 17 at 37,835,739 bp
  • A to T, chromosome 17 at 70,517,030 bp
  • A to T, chromosome 17 at 90,590,092 bp
  • T to C, chromosome 18 at 13,021,928 bp
  • C to A, chromosome 19 at 27,418,848 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5473 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043034-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.