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Strain Name:
C57BL/6J-MtgxR5474Btlr/Mmmh
Stock Number:
043035-MU
Citation ID:
RRID:MMRRC_043035-MU
Other Names:
R5474 (G1), C57BL/6J-MtgxR5474Btlr
Major Collection:

Strain Information

Ide
Name: insulin degrading enzyme
Synonyms: 1300012G03Rik, 4833415K22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 15925
HGNC: HGNC:5381
Homologene: 3645
Drd4
Name: dopamine receptor D4
Synonyms: Drd-4, D4R
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13491
HGNC: HGNC:3025
Homologene: 20215
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Kcnc4
Name: potassium voltage gated channel, Shaw-related subfamily, member 4
Synonyms: Kv3.4, Kcr2-4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99738
HGNC: HGNC:6236
Homologene: 68427
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: Gcap18, 4921514G19Rik, E430033I05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14479
VEGA: 10
Homologene: 101542
Prrc2c
Name: proline-rich coiled-coil 2C
Synonyms: 9630039I18Rik, 1810043M20Rik, Bat2d, Bat2l2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226562
Homologene: 41015
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238130
VEGA: 12
Homologene: 56680
Prrc2a
Name: proline-rich coiled-coil 2A
Synonyms: G2, Bat-2, D17H6S51E, 3110039B05Rik, Wbp12, Bat2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 53761
Homologene: 10567
Nemf
Name: nuclear export mediator factor
Synonyms: 4933405E14Rik, 1500011I12Rik, Sdccag1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66244
VEGA: 12
Homologene: 3458
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: 9030221A05Rik, A930018H20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320661
Homologene: 19716
Nrros
Name: negative regulator of reactive oxygen species
Synonyms: E430025L02Rik, Lrrc33
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224109
Homologene: 17134
Zfta
Name: zinc finger translocation associated
Synonyms: 2700081O15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 108899
Homologene: 52179
Scfd2
Name: Sec1 family domain containing 2
Synonyms: STXBP1L1, E430013M20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 212986
Homologene: 77411
Cngb1
Name: cyclic nucleotide gated channel beta 1
Synonyms: Cngb1, Cngb1b, BC016201
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 333329
HGNC: HGNC:2151
Homologene: 136420
Krt14
Name: keratin 14
Synonyms: epidermolysis bullosa simplex, Dowling-Meara, Koebner, Krt-1.14, K14, Cytokeratin 14, Krt1-14
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16664
HGNC: HGNC:6416
Homologene: 110439
Bud13
Name: BUD13 homolog
Synonyms: D030060M11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 215051
Homologene: 13102
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Slc22a29
Name: solute carrier family 22. member 29
Synonyms: D630002G06Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 236293
Homologene: 77136
Abcb5
Name: ATP-binding cassette, sub-family B member 5
Synonyms: 9230106F14Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 77706
HGNC: HGNC:46
Homologene: 83488
Cspg5
Name: chondroitin sulfate proteoglycan 5
Synonyms: NGC, neuroglycan C, CALEB
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 29873
HGNC: HGNC:2467
Homologene: 4795
Gtdc1
Name: glycosyltransferase-like domain containing 1
Synonyms: E330008O22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227835
Homologene: 32590
Or5w13
Name: olfactory receptor family 5 subfamily W member 13
Synonyms: GA_x6K02T2Q125-49193051-49192119, MOR177-3, Olfr1136
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258653
Homologene: 74193
Lrit1
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 1
Synonyms: Lrrc21
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239037
Homologene: 9215
Sec14l5
Name: SEC14-like lipid binding 5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 665119
VEGA: 16
Homologene: 96163
Cnga1
Name: cyclic nucleotide gated channel alpha 1
Synonyms: Cncg
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12788
HGNC: HGNC:2148
Homologene: 55432
Polb
Name: polymerase (DNA directed), beta
Synonyms: Pol beta, A430088C08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18970
HGNC: HGNC:9174
Homologene: 2013
Ankmy1
Name: ankyrin repeat and MYND domain containing 1
Synonyms: 4930483I10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241158
Homologene: 9561
Vmn2r17
Name: vomeronasal 2, receptor 17
Synonyms: EG384221
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384221
Homologene: 104825
H6pd
Name: hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
Synonyms: Gpd-1, Gpd1, G6pd1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100198
HGNC: HGNC:4795
Homologene: 48275
Cyp2c29
Name: cytochrome P450, family 2, subfamily c, polypeptide 29
Synonyms: AHOH, AHOHase, Ahh-1, Ah-2, P450-2C, Cyp2c
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13095
Homologene: 117948
Vav3
Name: vav 3 oncogene
Synonyms: A530094I06Rik, Idd18.1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 57257
Homologene: 38143
Zfp84
Name: zinc finger protein 84
Synonyms: C86188, Zfp69, KRAB18, 4633401C23Rik, 2210410P13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74352
Homologene: 51858
Clec4a4
Name: C-type lectin domain family 4, member a4
Synonyms: Dcir2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 474145
Homologene: 133910
Or6a2
Name: olfactory receptor family 6 subfamily A member 2
Synonyms: I54, I7, Olfr41, MOR103-15, GA_x6K02T2PBJ9-9381439-9380456, Olfr2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18317
Homologene: 2743
Ncs1
Name: neuronal calcium sensor 1
Synonyms: NCS-1, 9430075O15Rik, A730032G13Rik, Freq
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14299
HGNC: HGNC:3953
Homologene: 5719
H2-T3
Name: histocompatibility 2, T region locus 3
Synonyms: H-2T3, TL, H2-Tw3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15043
Homologene: 74884
Or8g51
Name: olfactory receptor family 8 subfamily G member 51
Synonyms: GA_x6K02T2PVTD-32400678-32399743, MOR171-23, Olfr919
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258432
VEGA: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 92,885,204 bp
  • T to C, chromosome 1 at 162,709,644 bp
  • A to T, chromosome 2 at 31,280,784 bp
  • A to T, chromosome 2 at 44,756,367 bp
  • G to A, chromosome 2 at 87,693,057 bp
  • A to T, chromosome 3 at 107,447,891 bp
  • A to G, chromosome 3 at 109,664,421 bp
  • A to G, chromosome 4 at 149,996,089 bp
  • G to T, chromosome 5 at 36,615,257 bp
  • T to C, chromosome 5 at 72,605,193 bp
  • C to T, chromosome 5 at 74,531,364 bp
  • G to A, chromosome 5 at 109,434,284 bp
  • T to C, chromosome 6 at 123,012,747 bp
  • C to T, chromosome 7 at 29,777,089 bp
  • T to A, chromosome 7 at 107,001,089 bp
  • T to C, chromosome 7 at 141,293,728 bp
  • A to G, chromosome 8 at 22,630,370 bp
  • A to T, chromosome 8 at 95,251,969 bp
  • T to A, chromosome 9 at 38,698,313 bp
  • G to C, chromosome 9 at 46,287,953 bp
  • A to T, chromosome 9 at 110,251,008 bp
  • C to T, chromosome 10 at 28,496,930 bp
  • T to C, chromosome 10 at 50,849,538 bp
  • T to C, chromosome 10 at 123,128,045 bp
  • C to T, chromosome 11 at 69,458,920 bp
  • A to T, chromosome 11 at 100,204,745 bp
  • T to C, chromosome 12 at 40,745,731 bp
  • C to A, chromosome 12 at 69,316,335 bp
  • C to T, chromosome 12 at 118,940,690 bp
  • T to A, chromosome 14 at 37,061,986 bp
  • A to G, chromosome 16 at 5,178,518 bp
  • T to C, chromosome 16 at 32,144,352 bp
  • G to A, chromosome 16 at 32,761,261 bp
  • A to T, chromosome 17 at 35,159,213 bp
  • G to A, chromosome 17 at 36,190,107 bp
  • A to T, chromosome 19 at 7,420,159 bp
  • A to G, chromosome 19 at 8,217,857 bp
  • A to G, chromosome 19 at 37,272,184 bp
  • G to A, chromosome 19 at 39,324,992 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5474 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043035-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.