Strain Name:
C57BL/6J-MtgxR5476Btlr/Mmmh
Stock Number:
043037-MU
Citation ID:
RRID:MMRRC_043037-MU
Other Names:
R5476 (G1), C57BL/6J-MtgxR5476Btlr
Major Collection:

Gene Information

Lefty1
Name: left right determination factor 1
Synonyms: Ebaf, lefty-1, Stra3, Lefty
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13590
HGNC: HGNC:6552
Homologene: 49231
Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 0610038M01Rik, 2610510B01Rik, Dopey2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Slc25a12
Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Synonyms: B230107K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78830
Homologene: 48235
Armc6
Name: armadillo repeat containing 6
Synonyms: 2410153K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76813
Homologene: 14121
Dock5
Name: dedicator of cytokinesis 5
Synonyms: 1110060D06Rik, lr2, rlc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Dock6
Name: dedicator of cytokinesis 6
Synonyms: 4931431C02Rik, C330023D02Rik, 2410095B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319899
VEGA: 9
Homologene: 83291
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Trappc8
Name: trafficking protein particle complex 8
Synonyms: 5033403J15Rik, D030074E01Rik, Trs85
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75964
VEGA: 18
Homologene: 40993
Wdr1
Name: WD repeat domain 1
Synonyms: D5Wsu185e, rede, Aip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22388
Homologene: 6628
Ddx6
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Synonyms: rck, C430015D01Rik, 1110001P04Rik, HLR2, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, E230023J21Rik, mRCK/P54, p54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13209
VEGA: 9
HGNC: HGNC:2747
Homologene: 3238
Rif1
Name: replication timing regulatory factor 1
Synonyms: 5730435J01Rik, 6530403D07Rik, D2Ertd145e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 51869
Homologene: 41231
Zfp442
Name: zinc finger protein 442
Synonyms: OTTMUSG00000015730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668923
Homologene: 136215
Chd8
Name: chromodomain helicase DNA binding protein 8
Synonyms: Duplin, 5830451P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67772
Homologene: 72405
Cep128
Name: centrosomal protein 128
Synonyms: 5430424K18Rik, 4930534B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 75216
Homologene: 35315
Aldh16a1
Name: aldehyde dehydrogenase 16 family, member A1
Synonyms: 2410004H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 69748
Homologene: 34938
Grm4
Name: glutamate receptor, metabotropic 4
Synonyms: Gprc1d, mGluR4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268934
HGNC: HGNC:4596
Homologene: 20230
Prr14l
Name: proline rich 14-like
Synonyms: Prl14l, C330019G07Rik, 6030436E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 215476
Homologene: 65866
Flvcr1
Name: feline leukemia virus subgroup C cellular receptor 1
Synonyms: 9630055N22Rik, Mfsd7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226844
Homologene: 56661
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: A930018H20Rik, 9030221A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320661
Homologene: 19716
Zfp157
Name: zinc finger protein 157
Synonyms: A630094N24Rik, 2610020C11Rik, Roma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72154
Homologene: 57009
Pbrm1
Name: polybromo 1
Synonyms: BAF180, Pb1, 2310032M22Rik, 2610016F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66923
Homologene: 10044
Phf21b
Name: PHD finger protein 21B
Synonyms: A730032D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 271305
Homologene: 78154
Pde4b
Name: phosphodiesterase 4B, cAMP specific
Synonyms: Dpde4, dunce
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18578
HGNC: HGNC:8781
Homologene: 1953
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: Ugcgl2, 3110001A05Rik, A230065J02Rik, 1810064L21Rik, 3110027P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66435
Homologene: 56875
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: Dnhd3, 2900022L05Rik, Dnahc2, D330014H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Snx13
Name: sorting nexin 13
Synonyms: RGS-PX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217463
VEGA: 12
Homologene: 41011
Mmp9
Name: matrix metallopeptidase 9
Synonyms: Gelatinase B, MMP-9, Clg4b, B/MMP9, gelatinase B, Gel B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17395
HGNC: HGNC:7176
Homologene: 3659
Ralgapa2
Name: Ral GTPase activating protein, alpha subunit 2 (catalytic)
Synonyms: RGC2, AS250, A230067G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241694
Homologene: 28131
Pard3b
Name: par-3 family cell polarity regulator beta
Synonyms: 1810008K04Rik, Als2cr19, 2810455B10Rik, PAR3beta, PAR3B, 2010002N16Rik, PAR3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72823
Homologene: 35389
Stpg2
Name: sperm tail PG rich repeat containing 2
Synonyms: B930007M17Rik, LOC381476
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381476
Homologene: 52167
Pik3r5
Name: phosphoinositide-3-kinase regulatory subunit 5
Synonyms: Foap2, p101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320207
Homologene: 8627
Smc1b
Name: structural maintenance of chromosomes 1B
Synonyms: Smc1l2, SMC1beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 140557
VEGA: 15
Homologene: 13786
Hps1
Name: HPS1, biogenesis of lysosomal organelles complex 3 subunit 1
Synonyms: 6030422N11Rik, Hermansky-Pudlak syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 192236
HGNC: HGNC:5163
Homologene: 163
Ccdc96
Name: coiled-coil domain containing 96
Synonyms: 4921513E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66717
Homologene: 32639
Mpp4
Name: membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Synonyms: DLG6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227157
Homologene: 23296
Spata2
Name: spermatogenesis associated 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 263876
Homologene: 4407
Cpox
Name: coproporphyrinogen oxidase
Synonyms: clone 560, cac, nct, Cpo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12892
VEGA: 16
HGNC: HGNC:2321
Homologene: 76
Nt5m
Name: 5',3'-nucleotidase, mitochondrial
Synonyms: dNT-2, 2010013E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103850
Homologene: 10622
Faim
Name: Fas apoptotic inhibitory molecule
Synonyms: Faim-S, Faim-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 23873
Homologene: 8018
Vmn1r175
Name: vomeronasal 1 receptor 175
Synonyms: Gm6299
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 622222
Homologene: 128341
Myl6b
Name: myosin, light polypeptide 6B
Synonyms: 5730437E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216459
VEGA: 10
Homologene: 86887
Alg5
Name: asparagine-linked glycosylation 5 (dolichyl-phosphate beta-glucosyltransferase)
Synonyms: 2600005J22Rik, 1500026A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66248
Homologene: 7060
Tor3a
Name: torsin family 3, member A
Synonyms: Adir
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 30935
Homologene: 23359
Pbp2
Name: phosphatidylethanolamine binding protein 2
Synonyms: Pebp2, 1700023A18Rik, Pebp-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76400
Homologene: 135930
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 59,158,662 bp
  • G to T, chromosome 1 at 62,010,406 bp
  • G to T, chromosome 1 at 156,673,567 bp
  • T to C, chromosome 1 at 180,937,698 bp
  • C to T, chromosome 1 at 191,011,619 bp
  • T to C, chromosome 2 at 20,880,686 bp
  • A to G, chromosome 2 at 52,089,595 bp
  • A to G, chromosome 2 at 71,275,322 bp
  • A to G, chromosome 2 at 146,447,436 bp
  • A to T, chromosome 2 at 150,408,159 bp
  • A to G, chromosome 2 at 164,952,494 bp
  • A to T, chromosome 2 at 167,484,159 bp
  • T to A, chromosome 3 at 54,746,598 bp
  • T to A, chromosome 3 at 139,243,138 bp
  • A to G, chromosome 4 at 102,602,699 bp
  • T to C, chromosome 5 at 32,844,138 bp
  • A to G, chromosome 5 at 36,485,637 bp
  • G to T, chromosome 5 at 36,615,257 bp
  • C to T, chromosome 5 at 38,529,588 bp
  • T to G, chromosome 5 at 138,457,181 bp
  • A to G, chromosome 6 at 135,309,924 bp
  • C to T, chromosome 7 at 23,809,131 bp
  • T to G, chromosome 7 at 45,142,069 bp
  • A to G, chromosome 8 at 70,225,042 bp
  • A to G, chromosome 9 at 21,809,589 bp
  • A to G, chromosome 9 at 44,607,456 bp
  • A to G, chromosome 9 at 98,992,729 bp
  • C to T, chromosome 10 at 128,497,347 bp
  • A to G, chromosome 11 at 59,875,907 bp
  • G to A, chromosome 11 at 68,495,370 bp
  • C to T, chromosome 11 at 69,458,920 bp
  • A to G, chromosome 12 at 35,106,820 bp
  • T to C, chromosome 12 at 91,213,618 bp
  • T to A, chromosome 14 at 31,032,519 bp
  • A to G, chromosome 14 at 52,207,300 bp
  • T to C, chromosome 14 at 67,814,007 bp
  • T to A, chromosome 14 at 119,090,709 bp
  • A to G, chromosome 15 at 84,787,265 bp
  • A to T, chromosome 15 at 85,086,151 bp
  • G to C, chromosome 16 at 58,678,725 bp
  • A to G, chromosome 16 at 93,773,913 bp
  • A to G, chromosome 17 at 27,434,798 bp
  • A to G, chromosome 18 at 20,865,108 bp
  • T to C, chromosome 19 at 42,769,602 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5476 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043037-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.