Strain Name:
C57BL/6J-MtgxR5477Btlr/Mmmh
Stock Number:
043038-MU
Citation ID:
RRID:MMRRC_043038-MU
Other Names:
R5477 (G1), C57BL/6J-MtgxR5477Btlr
Major Collection:

Gene Information

Pias3
Name: protein inhibitor of activated STAT 3
Synonyms: Pias3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229615
Homologene: 4447
Senp6
Name: SUMO/sentrin specific peptidase 6
Synonyms: E130319N12Rik, 2810017C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215351
Homologene: 9196
Rc3h2
Name: ring finger and CCCH-type zinc finger domains 2
Synonyms: Rnf164, D930043C02Rik, 2900024N03Rik, 9430019J22Rik, Mnab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319817
Homologene: 28276
Top2a
Name: topoisomerase (DNA) II alpha
Synonyms: DNA Topoisomerase II alpha, Top-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21973
Homologene: 830
Aff4
Name: AF4/FMR2 family, member 4
Synonyms: Alf4, Laf4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 93736
Homologene: 8683
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Iars2
Name: isoleucine-tRNA synthetase 2, mitochondrial
Synonyms: 2010002H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381314
Homologene: 7118
D5Ertd579e
Name: DNA segment, Chr 5, ERATO Doi 579, expressed
Synonyms: 9030221A05Rik, A930018H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320661
Homologene: 19716
Dmrt1
Name: doublesex and mab-3 related transcription factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 50796
HGNC: HGNC:2934
Homologene: 9280
Myo15
Name: myosin XV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17910
HGNC: HGNC:7594
Homologene: 56504
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268379
Homologene: 27991
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Tm4sf5
Name: transmembrane 4 superfamily member 5
Synonyms: 2010003F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75604
Homologene: 2947
9930021J03Rik
Name: RIKEN cDNA 9930021J03 gene
Synonyms: Gm9832
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 240613
Homologene: 19046
Sycp1
Name: synaptonemal complex protein 1
Synonyms: SCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20957
Homologene: 2389
Irak3
Name: interleukin-1 receptor-associated kinase 3
Synonyms: IRAK-M, 4833428C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73914
Homologene: 36215
Hs2st1
Name: heparan sulfate 2-O-sulfotransferase 1
Synonyms: Hs2st
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23908
HGNC: HGNC:5193
Homologene: 8025
Vmn1r124
Name: vomeronasal 1 receptor 124
Synonyms: Gm9500
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 670764
Homologene: 104166
Slc39a12
Name: solute carrier family 39 (zinc transporter), member 12
Synonyms: LOC277468
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277468
Homologene: 17654
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Pappa2
Name: pappalysin 2
Synonyms: placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E, PAPP-A2, PLAC3, Pappe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23850
Homologene: 10661
Ssbp2
Name: single-stranded DNA binding protein 2
Synonyms: Hspc116, 2310079I02Rik, 9330163K02Rik, 1500004K09Rik, A830008M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66970
Homologene: 22724
Parl
Name: presenilin associated, rhomboid-like
Synonyms: PSENIP2, PRO2207, PSARL1, D16Ertd607e, Psarl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 381038
Homologene: 10239
Olfr1347
Name: olfactory receptor 1347
Synonyms: GA_x6K02T2QGBW-3218686-3217748, MOR103-19_i, MOR103-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258383
Homologene: 128065
Spdl1
Name: spindle apparatus coiled-coil protein 1
Synonyms: 1700018I02Rik, 2810049B11Rik, 2600001J17Rik, Ccdc99
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70385
Homologene: 9837
Aacs
Name: acetoacetyl-CoA synthetase
Synonyms: 2210408B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 78894
Homologene: 11322
Sox7
Name: SRY (sex determining region Y)-box 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20680
VEGA: 14
Homologene: 7949
4930505A04Rik
Name: RIKEN cDNA 4930505A04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75087
Homologene: 18988
Olfr181
Name: olfactory receptor 181
Synonyms: GA_x54KRFPKG5P-55145984-55145034, MOR184-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 259001
Homologene: 74112
Slc27a3
Name: solute carrier family 27 (fatty acid transporter), member 3
Synonyms: fatty acid transport protein 3, FATP3, Acsvl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26568
Homologene: 11529
Trim30d
Name: tripartite motif-containing 30D
Synonyms: TRIM30-3, Trim79
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 209387
Homologene: 114426
Lats2
Name: large tumor suppressor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50523
HGNC: HGNC:6515
Homologene: 56678
Pskh1
Name: protein serine kinase H1
Synonyms: E130013P03Rik, b2b1230Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244631
HGNC: HGNC:9529
Homologene: 48461
Cln6
Name: ceroid-lipofuscinosis, neuronal 6
Synonyms: 1810065L06Rik, D9Bwg1455e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76524
HGNC: HGNC:2077
Homologene: 9898
Olfr178
Name: olfactory receptor 178
Synonyms: GA_x54KRFPKG5P-55108059-55107100, MOR184-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258999
Homologene: 79412
Gm10521
Name: predicted gene 10521
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
VEGA: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 158,956,738 bp
  • T to A, chromosome 1 at 171,896,500 bp
  • C to T, chromosome 1 at 185,288,155 bp
  • T to C, chromosome 2 at 14,389,382 bp
  • T to C, chromosome 2 at 37,399,630 bp
  • A to G, chromosome 3 at 90,386,839 bp
  • G to A, chromosome 3 at 96,705,003 bp
  • A to T, chromosome 3 at 102,818,890 bp
  • A to G, chromosome 3 at 144,556,948 bp
  • G to T, chromosome 5 at 36,615,257 bp
  • T to C, chromosome 5 at 125,511,920 bp
  • A to C, chromosome 6 at 48,498,393 bp
  • T to C, chromosome 7 at 6,488,571 bp
  • G to A, chromosome 7 at 21,259,728 bp
  • T to A, chromosome 7 at 104,472,140 bp
  • C to T, chromosome 8 at 105,929,879 bp
  • C to T, chromosome 9 at 62,846,920 bp
  • C to A, chromosome 9 at 80,143,843 bp
  • G to A, chromosome 10 at 120,182,587 bp
  • A to G, chromosome 11 at 9,301,298 bp
  • C to T, chromosome 11 at 30,426,349 bp
  • A to T, chromosome 11 at 34,822,210 bp
  • T to A, chromosome 11 at 53,408,472 bp
  • A to C, chromosome 11 at 60,477,677 bp
  • C to T, chromosome 11 at 69,458,920 bp
  • C to T, chromosome 11 at 70,510,348 bp
  • T to A, chromosome 11 at 99,016,480 bp
  • G to T, chromosome 13 at 11,705,656 bp
  • T to A, chromosome 13 at 91,664,125 bp
  • T to C, chromosome 14 at 57,699,553 bp
  • A to G, chromosome 14 at 63,948,496 bp
  • G to T, chromosome 16 at 20,280,074 bp
  • T to A, chromosome 16 at 58,889,744 bp
  • A to T, chromosome 16 at 58,926,030 bp
  • A to G, chromosome 19 at 25,509,800 bp
  • A to G, chromosome 19 at 29,754,118 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5477 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043038-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.