Strain Name:
C57BL/6J-MtgxR5478Btlr/Mmmh
Stock Number:
043039-MU
Citation ID:
RRID:MMRRC_043039-MU
Other Names:
R5478 (G1), C57BL/6J-MtgxR5478Btlr
Major Collection:

Strain Information

Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: 1110037D04Rik, Lrrc16, Carmil, Lrrc16a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68732
Homologene: 9757
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Rspry1
Name: ring finger and SPRY domain containing 1
Synonyms: 4930470D19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67610
Homologene: 12164
Amotl1
Name: angiomotin-like 1
Synonyms: 4932416D09Rik, JEAP, 2310067L22Rik, 2310010G08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75723
Homologene: 43977
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230895
Homologene: 15583
Srpk2
Name: serine/arginine-rich protein specific kinase 2
Synonyms: mSRPK2, WBP6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20817
Homologene: 101663
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, 5830472H07Rik, A130095G14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Cdkl2
Name: cyclin dependent kinase like 2
Synonyms: Kkm, KKIAMRE, 5330436L21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53886
HGNC: HGNC:1782
Homologene: 55793
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75619
Homologene: 8957
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: tigmin, FPC
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Mmrn2
Name: multimerin 2
Synonyms: EndoGlyx-1, ENDOGLYX1, Emilin3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105450
VEGA: 14
Homologene: 11697
Slc9c1
Name: solute carrier family 9, subfamily C (Na+-transporting carboxylic acid decarboxylase), member 1
Synonyms: LOC208169, spermNHE, Slc9a10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208169
Homologene: 19505
Epha3
Name: Eph receptor A3
Synonyms: Tyro4, Mek4, Hek4, Hek, Cek4, End3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13837
VEGA: 16
HGNC: HGNC:3387
Homologene: 21083
Slc4a1
Name: solute carrier family 4 (anion exchanger), member 1
Synonyms: erythrocyte membrane protein band 3, band 3, Empb3, Ae1, CD233, l11Jus51
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20533
Homologene: 133556
Per2
Name: period circadian clock 2
Synonyms: mPer2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18627
HGNC: HGNC:8846
Homologene: 7885
Ppargc1b
Name: peroxisome proliferative activated receptor, gamma, coactivator 1 beta
Synonyms: PGC-1beta/ERRL1, 4631412G21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 170826
Homologene: 15776
Or5h17
Name: olfactory receptor family 5 subfamily H member 17
Synonyms: GA_x54KRFPKG5P-55229158-55230087, MOR183-2, Olfr183
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258478
Homologene: 27178
Adamts2
Name: ADAM metallopeptidase with thrombospondin type 1 motif 2
Synonyms: ADAM-TS2, hPCPNI, a disintegrin and metalloproteinase with thrombospondin repeats, procollagen N-proteinase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216725
HGNC: HGNC:218
Homologene: 8597
Capn3
Name: calpain 3
Synonyms: Capa-3, Capa3, Lp82, p94
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12335
HGNC: HGNC:1480
Homologene: 52
Fshr
Name: follicle stimulating hormone receptor
Synonyms: follicle-stimulating hormone receptor, Follitropin receptor, FSH-R
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14309
VEGA: 17
HGNC: HGNC:3969
Homologene: 117
Chl1
Name: cell adhesion molecule L1-like
Synonyms: CALL, A530023M13Rik, close homolog of L1, LICAM2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12661
HGNC: HGNC:1939
Homologene: 21314
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620928
Homologene: 115466
Col4a2
Name: collagen, type IV, alpha 2
Synonyms: Col4a-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12827
HGNC: HGNC:2203
Homologene: 1390
Tbc1d16
Name: TBC1 domain family, member 16
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207592
Homologene: 10380
Gm4922
Name: predicted gene 4922
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237300
VEGA: 10
Homologene: 50497
Osmr
Name: oncostatin M receptor
Synonyms: OSMRB
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18414
HGNC: HGNC:8507
Homologene: 2972
Abcc2
Name: ATP-binding cassette, sub-family member 2
Synonyms: multidrug resistance protein 2, Mrp2, Cmoat
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12780
HGNC: HGNC:53
Homologene: 68052
Hnf4a
Name: hepatic nuclear factor 4, alpha
Synonyms: HNF-4, Nr2a1, Nuclear receptor 2A1, HNF4 alpha, Tcf14, MODY1, Tcf4, Hnf4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15378
HGNC: HGNC:5024
Homologene: 395
Cdhr4
Name: cadherin-related family member 4
Synonyms: D330022A01Rik, 1700021K14Rik, Cdh29
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69398
Homologene: 82362
Defa35
Name: defensin, alpha, 35
Synonyms: ENSMUSG00000061845, OTTMUSG00000018258, Gm10104
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100041688
Homologene: 113382
Idh1
Name: isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms: Id-1, Idh-1, E030024J03Rik, IDPc
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 15926
HGNC: HGNC:5382
Homologene: 21195
Veph1
Name: ventricular zone expressed PH domain-containing 1
Synonyms: Veph, 2810471M23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72789
Homologene: 11624
Plaat5
Name: phospholipase A and acyltransferase 5
Synonyms: 1700086E08Rik, 1700055A14Rik, 4921526K24Rik, iNAT, Hrasls5
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66727
Homologene: 12019
Ugt2b36
Name: UDP glucuronosyltransferase 2 family, polypeptide B36
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231396
Homologene: 128251
Apba2
Name: amyloid beta precursor protein binding family A member 2
Synonyms: X11-like, X11L, Mint 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11784
HGNC: HGNC:579
Homologene: 4021
BC048562
Name: cDNA sequence BC048562
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 434439
Homologene: 79041
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71481
Homologene: 11849
Prpf18
Name: pre-mRNA processing factor 18
Synonyms: 2810441A10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67229
Homologene: 2726
Pnliprp1
Name: pancreatic lipase related protein 1
Synonyms: Plrp1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18946
VEGA: 19
HGNC: HGNC:9156
Homologene: 21253
Tktl2
Name: transketolase-like 2
Synonyms: 4933401I19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 74419
Homologene: 69456
Or52ab7
Name: olfactory receptor family 52 subfamily AB member 7
Synonyms: GA_x6K02T2PBJ9-6037823-6038782, MOR23-4P, MOR23-5, Olfr598
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257975
Homologene: 132401
Krt222
Name: keratin 222
Synonyms: 6330509G02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268481
Homologene: 17556
Ctsm
Name: cathepsin M
Synonyms: Cat M, Catm, 1600027J17Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 64139
VEGA: 13
Homologene: 75181
Slc26a10
Name: solute carrier family 26, member 10
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216441
VEGA: 10
Homologene: 66953
Slc25a23
Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 23
Synonyms: 2310067G05Rik, SCaMC-3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66972
Homologene: 11467
Rnase11
Name: ribonuclease, RNase A family, 11 (non-active)
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 497113
Homologene: 17054
Pdrg1
Name: p53 and DNA damage regulated 1
Synonyms: 1110004D19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68559
Homologene: 12779
Spmip8
Name: sperm microtubule inner protein 8
Synonyms: 1700055M20Rik, Tepp
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73407
Homologene: 28311
Gm16686
Name: predicted gene, 16686
Type: Gene
Species: Mouse
Chromosome: 4
Gm5709
Name: predicted gene 5709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 435732
Homologene: 33954
Pcdhga8
Name: protocadherin gamma subfamily A, 8
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93716
HGNC: HGNC:8706
Homologene: 57162
A430057M04Rik
Name: RIKEN cDNA A430057M04 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 319486
Gm26637
Name: predicted gene, 26637
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 105246320
VEGA: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 20,201,156 bp
  • T to C, chromosome 1 at 63,739,186 bp
  • A to T, chromosome 1 at 65,161,838 bp
  • T to A, chromosome 1 at 91,432,868 bp
  • A to T, chromosome 2 at 4,638,894 bp
  • A to G, chromosome 2 at 120,464,185 bp
  • A to G, chromosome 2 at 153,015,232 bp
  • A to T, chromosome 2 at 163,569,006 bp
  • A to T, chromosome 3 at 59,635,674 bp
  • T to C, chromosome 3 at 66,255,022 bp
  • A to G, chromosome 3 at 127,677,719 bp
  • C to A, chromosome 4 at 49,792,481 bp
  • A to T, chromosome 4 at 88,755,477 bp
  • T to A, chromosome 4 at 130,751,484 bp
  • G to A, chromosome 4 at 145,167,550 bp
  • A to T, chromosome 5 at 22,004,203 bp
  • G to A, chromosome 5 at 23,524,183 bp
  • A to T, chromosome 5 at 87,089,482 bp
  • T to A, chromosome 5 at 92,039,249 bp
  • A to G, chromosome 6 at 39,677,574 bp
  • A to T, chromosome 6 at 103,683,221 bp
  • T to A, chromosome 7 at 64,695,186 bp
  • A to T, chromosome 7 at 85,869,788 bp
  • T to C, chromosome 7 at 103,328,825 bp
  • T to A, chromosome 8 at 11,398,697 bp
  • A to T, chromosome 8 at 21,065,820 bp
  • T to C, chromosome 8 at 66,513,398 bp
  • T to A, chromosome 8 at 94,629,778 bp
  • A to G, chromosome 8 at 95,325,565 bp
  • A to G, chromosome 9 at 14,592,752 bp
  • G to A, chromosome 9 at 107,995,591 bp
  • G to A, chromosome 9 at 108,445,164 bp
  • T to C, chromosome 10 at 18,784,137 bp
  • C to A, chromosome 10 at 127,173,949 bp
  • T to C, chromosome 11 at 50,792,651 bp
  • G to T, chromosome 11 at 65,233,088 bp
  • A to G, chromosome 11 at 99,234,948 bp
  • T to A, chromosome 11 at 102,350,314 bp
  • T to C, chromosome 11 at 119,155,091 bp
  • T to C, chromosome 13 at 24,112,045 bp
  • A to T, chromosome 13 at 61,537,729 bp
  • A to T, chromosome 14 at 21,848,913 bp
  • A to T, chromosome 14 at 34,396,582 bp
  • A to G, chromosome 14 at 51,049,875 bp
  • T to C, chromosome 15 at 6,842,808 bp
  • A to T, chromosome 16 at 45,554,246 bp
  • A to T, chromosome 16 at 59,000,062 bp
  • A to C, chromosome 16 at 63,583,533 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • T to A, chromosome 17 at 57,052,780 bp
  • A to T, chromosome 17 at 78,894,672 bp
  • T to C, chromosome 17 at 80,433,847 bp
  • C to T, chromosome 17 at 89,001,715 bp
  • C to T, chromosome 18 at 37,817,322 bp
  • T to A, chromosome 18 at 61,307,568 bp
  • A to C, chromosome 19 at 7,614,671 bp
  • T to A, chromosome 19 at 25,079,822 bp
  • T to C, chromosome 19 at 43,839,465 bp
  • A to T, chromosome 19 at 58,734,991 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5478 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043039-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.