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Strain Name:
C57BL/6J-MtgxR5478Btlr/Mmmh
Stock Number:
043039-MU
Citation ID:
RRID:MMRRC_043039-MU
Other Names:
R5478 (G1), C57BL/6J-MtgxR5478Btlr
Major Collection:

Strain Information

Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: 1110037D04Rik, Lrrc16, Carmil, Lrrc16a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68732
Homologene: 9757
Braf
Name: Braf transforming gene
Synonyms: Braf-2, Braf2, 9930012E13Rik, D6Ertd631e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109880
HGNC: HGNC:1097
Homologene: 3197
Rspry1
Name: ring finger and SPRY domain containing 1
Synonyms: 4930470D19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67610
Homologene: 12164
Amotl1
Name: angiomotin-like 1
Synonyms: 4932416D09Rik, JEAP, 2310067L22Rik, 2310010G08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75723
Homologene: 43977
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 20,201,156 bp
  • T to C, chromosome 1 at 63,739,186 bp
  • A to T, chromosome 1 at 65,161,838 bp
  • T to A, chromosome 1 at 91,432,868 bp
  • A to T, chromosome 2 at 4,638,894 bp
  • A to G, chromosome 2 at 120,464,185 bp
  • A to G, chromosome 2 at 153,015,232 bp
  • A to T, chromosome 2 at 163,569,006 bp
  • A to T, chromosome 3 at 59,635,674 bp
  • T to C, chromosome 3 at 66,255,022 bp
  • A to G, chromosome 3 at 127,677,719 bp
  • C to A, chromosome 4 at 49,792,481 bp
  • A to T, chromosome 4 at 88,755,477 bp
  • T to A, chromosome 4 at 130,751,484 bp
  • G to A, chromosome 4 at 145,167,550 bp
  • A to T, chromosome 5 at 22,004,203 bp
  • G to A, chromosome 5 at 23,524,183 bp
  • A to T, chromosome 5 at 87,089,482 bp
  • T to A, chromosome 5 at 92,039,249 bp
  • A to G, chromosome 6 at 39,677,574 bp
  • A to T, chromosome 6 at 103,683,221 bp
  • T to A, chromosome 7 at 64,695,186 bp
  • A to T, chromosome 7 at 85,869,788 bp
  • T to C, chromosome 7 at 103,328,825 bp
  • T to A, chromosome 8 at 11,398,697 bp
  • A to T, chromosome 8 at 21,065,820 bp
  • T to C, chromosome 8 at 66,513,398 bp
  • T to A, chromosome 8 at 94,629,778 bp
  • A to G, chromosome 8 at 95,325,565 bp
  • A to G, chromosome 9 at 14,592,752 bp
  • G to A, chromosome 9 at 107,995,591 bp
  • G to A, chromosome 9 at 108,445,164 bp
  • T to C, chromosome 10 at 18,784,137 bp
  • C to A, chromosome 10 at 127,173,949 bp
  • T to C, chromosome 11 at 50,792,651 bp
  • G to T, chromosome 11 at 65,233,088 bp
  • A to G, chromosome 11 at 99,234,948 bp
  • T to A, chromosome 11 at 102,350,314 bp
  • T to C, chromosome 11 at 119,155,091 bp
  • T to C, chromosome 13 at 24,112,045 bp
  • A to T, chromosome 13 at 61,537,729 bp
  • A to T, chromosome 14 at 21,848,913 bp
  • A to T, chromosome 14 at 34,396,582 bp
  • A to G, chromosome 14 at 51,049,875 bp
  • T to C, chromosome 15 at 6,842,808 bp
  • A to T, chromosome 16 at 45,554,246 bp
  • A to T, chromosome 16 at 59,000,062 bp
  • A to C, chromosome 16 at 63,583,533 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • T to A, chromosome 17 at 57,052,780 bp
  • A to T, chromosome 17 at 78,894,672 bp
  • T to C, chromosome 17 at 80,433,847 bp
  • C to T, chromosome 17 at 89,001,715 bp
  • C to T, chromosome 18 at 37,817,322 bp
  • T to A, chromosome 18 at 61,307,568 bp
  • A to C, chromosome 19 at 7,614,671 bp
  • T to A, chromosome 19 at 25,079,822 bp
  • T to C, chromosome 19 at 43,839,465 bp
  • A to T, chromosome 19 at 58,734,991 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5478 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043039-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.