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Strain Name:
C57BL/6J-MtgxR5481Btlr/Mmmh
Stock Number:
043042-MU
Citation ID:
RRID:MMRRC_043042-MU
Other Names:
R5481 (G1), C57BL/6J-MtgxR5481Btlr
Major Collection:

Strain Information

Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Fam3c
Name: FAM3 metabolism regulating signaling molecule C
Synonyms: Fam3c, D6Wsu176e, Ilei
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27999
Homologene: 8926
Manba
Name: mannosidase, beta A, lysosomal
Synonyms: Bmn, 2410030O07Rik, B930014J03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110173
HGNC: HGNC:6831
Homologene: 4317
Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894
Barhl1
Name: BarH like homeobox 1
Synonyms: Dres115, MBH2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 54422
HGNC: HGNC:953
Homologene: 81871
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,471,663 bp
  • T to C, chromosome 1 at 93,060,244 bp
  • T to C, chromosome 1 at 120,241,724 bp
  • A to G, chromosome 1 at 139,457,061 bp
  • A to G, chromosome 2 at 28,915,340 bp
  • C to A, chromosome 2 at 31,188,706 bp
  • C to A, chromosome 2 at 48,940,386 bp
  • T to A, chromosome 2 at 73,566,926 bp
  • T to C, chromosome 2 at 82,979,886 bp
  • A to T, chromosome 2 at 91,572,447 bp
  • T to C, chromosome 2 at 120,728,393 bp
  • C to A, chromosome 2 at 164,506,422 bp
  • A to T, chromosome 3 at 88,453,040 bp
  • A to G, chromosome 3 at 92,429,351 bp
  • G to A, chromosome 3 at 125,683,193 bp
  • A to T, chromosome 3 at 135,524,556 bp
  • G to T, chromosome 3 at 138,325,958 bp
  • A to G, chromosome 4 at 107,060,579 bp
  • A to G, chromosome 4 at 108,644,349 bp
  • A to G, chromosome 4 at 109,505,562 bp
  • A to T, chromosome 5 at 24,589,342 bp
  • A to G, chromosome 5 at 108,648,810 bp
  • T to C, chromosome 5 at 108,801,770 bp
  • A to G, chromosome 5 at 110,649,069 bp
  • A to T, chromosome 5 at 117,867,754 bp
  • A to G, chromosome 5 at 122,680,820 bp
  • T to A, chromosome 5 at 129,514,818 bp
  • A to G, chromosome 5 at 138,194,816 bp
  • A to T, chromosome 5 at 150,260,319 bp
  • T to C, chromosome 6 at 22,321,358 bp
  • C to A, chromosome 6 at 23,113,476 bp
  • G to A, chromosome 6 at 29,441,217 bp
  • T to A, chromosome 6 at 87,050,969 bp
  • A to C, chromosome 7 at 26,766,821 bp
  • G to A, chromosome 7 at 127,532,197 bp
  • T to A, chromosome 7 at 128,442,447 bp
  • A to G, chromosome 8 at 80,993,323 bp
  • T to C, chromosome 8 at 83,319,594 bp
  • A to G, chromosome 8 at 119,599,240 bp
  • A to T, chromosome 9 at 38,657,620 bp
  • A to C, chromosome 9 at 95,977,914 bp
  • T to C, chromosome 9 at 111,229,837 bp
  • A to G, chromosome 10 at 34,395,664 bp
  • A to G, chromosome 10 at 75,735,066 bp
  • A to G, chromosome 10 at 127,647,826 bp
  • T to C, chromosome 10 at 128,572,480 bp
  • A to G, chromosome 11 at 30,935,378 bp
  • A to T, chromosome 11 at 50,878,071 bp
  • C to A, chromosome 11 at 54,502,644 bp
  • A to G, chromosome 11 at 80,663,095 bp
  • A to G, chromosome 11 at 96,107,543 bp
  • A to G, chromosome 11 at 99,985,327 bp
  • T to C, chromosome 13 at 12,188,155 bp
  • T to C, chromosome 13 at 30,946,959 bp
  • T to C, chromosome 13 at 33,038,093 bp
  • A to G, chromosome 14 at 31,308,871 bp
  • C to A, chromosome 14 at 48,252,633 bp
  • A to T, chromosome 14 at 56,373,389 bp
  • A to G, chromosome 15 at 44,558,646 bp
  • A to C, chromosome 15 at 76,691,021 bp
  • A to T, chromosome 15 at 96,420,617 bp
  • A to G, chromosome 15 at 98,862,005 bp
  • T to A, chromosome 16 at 23,224,168 bp
  • T to A, chromosome 16 at 34,921,604 bp
  • T to A, chromosome 16 at 48,561,485 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • C to A, chromosome 16 at 93,862,655 bp
  • C to A, chromosome 17 at 13,516,888 bp
  • C to T, chromosome 17 at 25,201,172 bp
  • T to C, chromosome 17 at 66,118,865 bp
  • A to C, chromosome 18 at 33,205,245 bp
  • T to C, chromosome 19 at 8,336,837 bp
  • A to G, chromosome 19 at 10,200,658 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5481 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043042-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.