Strain Name:
C57BL/6J-MtgxR5486Btlr/Mmmh
Stock Number:
043047-MU
Citation ID:
RRID:MMRRC_043047-MU
Other Names:
R5486 (G1), C57BL/6J-MtgxR5486Btlr
Major Collection:

Gene Information

Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: Edd1, 4432411E13Rik, Edd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin, Frabin-alpha, Frabin-beta, Frabin-gamma, ZFYVE6, 9330209B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224014
Homologene: 26727
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, b2b1625.2Clo, D230004K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Smg7
Name: Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms: 9430023P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226517
Homologene: 32235
Arhgap21
Name: Rho GTPase activating protein 21
Synonyms: ARHGAP10, 5530401C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71435
Homologene: 10822
Snrnp200
Name: small nuclear ribonucleoprotein 200 (U5)
Synonyms: A330064G03Rik, U5-200KD, Ascc3l1, HELIC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320632
Homologene: 5859
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: P1, p1.m, Mcmd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Fam168a
Name: family with sequence similarity 168, member A
Synonyms: 2610030B18Rik, B930006L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 319604
Homologene: 18702
Thoc1
Name: THO complex 1
Synonyms: NMP-84, 3110002N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225160
VEGA: 18
Homologene: 38012
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, G630013P12Rik, B130055N23Rik, Tfnr, TFC5, TAF3B1, TFIIIB90
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Clic6
Name: chloride intracellular channel 6
Synonyms: 5730466J16Rik, CLIC1L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209195
HGNC: HGNC:2065
Homologene: 43154
Pld3
Name: phospholipase D family, member 3
Synonyms: Sam-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18807
Homologene: 7893
Bod1l
Name: biorientation of chromosomes in cell division 1-like
Synonyms: A230054D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 665775
Homologene: 45109
Adam12
Name: a disintegrin and metallopeptidase domain 12 (meltrin alpha)
Synonyms: ADAM12, Mltna
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11489
HGNC: HGNC:190
Homologene: 74862
Hpcal4
Name: hippocalcin-like 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 170638
Homologene: 9441
Add3
Name: adducin 3 (gamma)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 27360
VEGA: 19
HGNC: HGNC:245
Homologene: 40893
Lbr
Name: lamin B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98386
HGNC: HGNC:6518
Homologene: 2455
Tecpr2
Name: tectonin beta-propeller repeat containing 2
Synonyms: 4930573I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104859
VEGA: 12
Homologene: 8897
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Fussel18, Gm7348, Corl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 664805
VEGA: 18
Homologene: 64797
Wdr95
Name: WD40 repeat domain 95
Synonyms: 4930434E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381693
Homologene: 124480
4932431P20Rik
Name: RIKEN cDNA 4932431P20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, Fath2, EMI2, mKIAA0811
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Sclt1
Name: sodium channel and clathrin linker 1
Synonyms: Tg(ACTB-sb10)1Dla, CAGGS-SB10, Tg(CAG-sb10)1Dla, 2610207F23Rik, 4931421F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67161
Homologene: 27031
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225638
Homologene: 50475
Olfr646
Name: olfactory receptor 646
Synonyms: GA_x6K02T2PBJ9-6841330-6842268, MOR33-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259058
Homologene: 17481
Nr1d2
Name: nuclear receptor subfamily 1, group D, member 2
Synonyms: Rev-erb beta, RVR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 353187
HGNC: HGNC:7963
Homologene: 3763
Olfr853
Name: olfactory receptor 853
Synonyms: GA_x6K02T2PVTD-13277703-13276786, MOR154-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258908
HGNC: HGNC:8466
Homologene: 133623
Piwil2
Name: piwi-like RNA-mediated gene silencing 2
Synonyms: mili, Miwi like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 57746
VEGA: 14
Homologene: 23071
Sh2b2
Name: SH2B adaptor protein 2
Synonyms: Aps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23921
Homologene: 10309
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228432
Homologene: 57147
Olfr665
Name: olfactory receptor 665
Synonyms: MOR34-7, GA_x6K02T2PBJ9-7509539-7510489
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258810
Homologene: 110477
Slc22a22
Name: solute carrier family 22 (organic cation transporter), member 22
Synonyms: BC026439, OAT-PG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 210463
Homologene: 18166
Cyp2d9
Name: cytochrome P450, family 2, subfamily d, polypeptide 9
Synonyms: Cyp2d, P450-2D, testosterone 16alpha-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13105
VEGA: 15
Homologene: 86099
Taar7a
Name: trace amine-associated receptor 7A
Synonyms: Taar7a, LOC215856
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215856
Homologene: 110833
Ccdc7a
Name: coiled-coil domain containing 7A
Synonyms: 4930540C21Rik, 4930517G15Rik, Ccdc7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74703
Homologene: 134760
Dnajb12
Name: DnaJ heat shock protein family (Hsp40) member B12
Synonyms: mDj10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56709
Homologene: 9749
Cln5
Name: ceroid-lipofuscinosis, neuronal 5
Synonyms: A730075N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 211286
VEGA: 14
HGNC: HGNC:2076
Homologene: 4738
Erlec1
Name: endoplasmic reticulum lectin 1
Synonyms: 4933407N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66753
Homologene: 9247
Tex19.2
Name: testis expressed gene 19.2
Synonyms: Tex19.2, 4921530G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70956
Homologene: 132323
Acad8
Name: acyl-Coenzyme A dehydrogenase family, member 8
Synonyms: 2310016C19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66948
HGNC: HGNC:87
Homologene: 8662
A930009A15Rik
Name: RIKEN cDNA A930009A15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77798
Pim3
Name: proviral integration site 3
Synonyms: Kid1, KID-1/kinase induced by depolarization
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223775
VEGA: 15
Homologene: 56955
Gm21972
Name: predicted gene 21972
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Xntrpc
Name: Xndc1-transient receptor potential cation channel, subfamily C, member 2 readthrough
Synonyms: Xndr-trpc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102443351
Homologene: 134407
Gm23482
Name: predicted gene, 23482
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 20,814,894 bp
  • A to G, chromosome 1 at 86,137,050 bp
  • A to G, chromosome 1 at 152,846,176 bp
  • A to G, chromosome 1 at 181,818,838 bp
  • T to A, chromosome 2 at 20,855,656 bp
  • T to C, chromosome 2 at 69,437,465 bp
  • T to C, chromosome 2 at 110,745,870 bp
  • C to T, chromosome 2 at 127,233,066 bp
  • A to G, chromosome 3 at 41,647,471 bp
  • A to G, chromosome 4 at 123,190,764 bp
  • A to T, chromosome 5 at 41,807,181 bp
  • A to G, chromosome 5 at 136,232,090 bp
  • A to T, chromosome 5 at 149,596,330 bp
  • C to A, chromosome 7 at 27,533,731 bp
  • T to G, chromosome 7 at 29,536,848 bp
  • T to A, chromosome 7 at 100,834,169 bp
  • GTGTCCTA to GTGTCCTATGTCCTA, chromosome 7 at 102,095,213 bp
  • C to A, chromosome 7 at 104,106,498 bp
  • T to A, chromosome 7 at 104,880,961 bp
  • C to A, chromosome 7 at 133,907,672 bp
  • T to C, chromosome 8 at 128,985,403 bp
  • A to G, chromosome 9 at 19,537,294 bp
  • A to T, chromosome 9 at 26,999,495 bp
  • T to A, chromosome 10 at 23,992,458 bp
  • GC to G, chromosome 10 at 59,892,752 bp
  • G to T, chromosome 10 at 115,579,905 bp
  • A to T, chromosome 11 at 30,935,047 bp
  • A to T, chromosome 11 at 55,253,681 bp
  • A to T, chromosome 11 at 121,117,478 bp
  • A to T, chromosome 12 at 110,933,015 bp
  • T to A, chromosome 13 at 49,709,573 bp
  • T to C, chromosome 13 at 100,098,510 bp
  • A to G, chromosome 14 at 18,206,860 bp
  • T to C, chromosome 14 at 70,401,431 bp
  • T to C, chromosome 14 at 103,076,194 bp
  • C to A, chromosome 15 at 38,008,739 bp
  • A to G, chromosome 15 at 57,263,451 bp
  • T to A, chromosome 15 at 82,452,578 bp
  • T to C, chromosome 15 at 88,863,222 bp
  • A to T, chromosome 16 at 16,475,037 bp
  • A to G, chromosome 16 at 92,529,852 bp
  • A to G, chromosome 18 at 9,992,204 bp
  • A to T, chromosome 18 at 65,294,354 bp
  • A to G, chromosome 18 at 76,858,700 bp
  • G to A, chromosome 19 at 53,244,387 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5486 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043047-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.