Strain Name:
C57BL/6J-MtgxR5491Btlr/Mmmh
Stock Number:
043052-MU
Citation ID:
RRID:MMRRC_043052-MU
Other Names:
R5491 (G1), C57BL/6J-MtgxR5491Btlr
Major Collection:

Strain Information

Neurod4
Name: neurogenic differentiation 4
Synonyms: MATH-3, Math3, Atoh3, bHLHa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11923
VEGA: 10
Homologene: 7234
Phf20l1
Name: PHD finger protein 20-like 1
Synonyms: CGI-72, E130113K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239510
VEGA: 15
Homologene: 9341
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103554
Homologene: 113742
Ncaph
Name: non-SMC condensin I complex, subunit H
Synonyms: HCAP-H, A730011O11Rik, Brrn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 215387
HGNC: HGNC:1112
Homologene: 133986
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108989
Homologene: 37753
Gpat4
Name: glycerol-3-phosphate acyltransferase 4
Synonyms: Agpat6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102247
Homologene: 32425
Pten
Name: phosphatase and tensin homolog
Synonyms: MMAC1, TEP1, 2310035O07Rik, A130070J02Rik, B430203M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19211
HGNC: HGNC:9588
Homologene: 265
Cisd2
Name: CDGSH iron sulfur domain 2
Synonyms: 1500031D15Rik, 1500026J14Rik, B630006A20Rik, 1500009M05Rik, Miner1, Zcd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67006
Homologene: 36436
Rassf1
Name: Ras association (RalGDS/AF-6) domain family member 1
Synonyms: 123F protein, REH3P21, NORE2A, RDA32, Rassf1C, Rassf1B, Rassf1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56289
HGNC: HGNC:9882
Homologene: 10499
Shisal1
Name: shisa like 1
Synonyms: 1810041L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72301
Homologene: 28255
Bach2
Name: BTB and CNC homology, basic leucine zipper transcription factor 2
Synonyms: E030004N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12014
Homologene: 7240
Zfp60
Name: zinc finger protein 60
Synonyms: Mfg-3, Mfg3, 6330516O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22718
Homologene: 137228
Fbxo7
Name: F-box protein 7
Synonyms: 2410015K21Rik, A230052G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69754
Homologene: 8136
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Gm12695
Name: predicted gene 12695
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 620779
Homologene: 51847
Col6a6
Name: collagen, type VI, alpha 6
Synonyms: E330026B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 245026
Homologene: 18260
Nebl
Name: nebulette
Synonyms: Lnebl, 1200007O21Rik, A630080F05Rik, D830029A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74103
Homologene: 128431
Cd248
Name: CD248 antigen, endosialin
Synonyms: 2610111G01Rik, Tem1, Cd164l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 70445
VEGA: 19
Homologene: 10699
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218989
Homologene: 49506
Eif4a3l1
Name: eukaryotic translation initiation factor 4A3 like 1
Synonyms: Gm8994, B020013A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 668137
Garin5b
Name: golgi associated RAB2 interactor family member 5B
Synonyms: 4930401F20Rik, Fam71e2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243822
Homologene: 89225
Aldh1l2
Name: aldehyde dehydrogenase 1 family, member L2
Synonyms: D330038I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216188
Homologene: 51942
A1cf
Name: APOBEC1 complementation factor
Synonyms: apobec-1 complementation factor, ACF, 1810073H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 69865
VEGA: 19
Homologene: 16363
Or3a1d
Name: olfactory receptor family 3 subfamily A member 1D
Synonyms: GA_x6K02T2P1NL-4481525-4480578, MOR255-3, Olfr411
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258704
Homologene: 74999
Phactr2
Name: phosphatase and actin regulator 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215789
Homologene: 8816
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229534
Homologene: 10740
Or13j1
Name: olfactory receptor family 13 subfamily J member 1
Synonyms: mOR17, MOR262-4, GA_x6K02T2N78B-16230286-16231224, Olfr71
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56015
Homologene: 10460
She
Name: src homology 2 domain-containing transforming protein E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214547
Homologene: 18186
Cela1
Name: chymotrypsin-like elastase family, member 1
Synonyms: PC-TsF, Ela-1, 1810009A17Rik, 1810062B19Rik, Ela1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 109901
VEGA: 15
HGNC: HGNC:3308
Homologene: 20454
Or4f56
Name: olfactory receptor family 4 subfamily F member 56
Synonyms: GA_x6K02T2Q125-72930843-72929905, MOR245-8, Olfr1305
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258396
Homologene: 74056
Fbxo48
Name: F-box protein 48
Synonyms: A630050E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319701
Homologene: 18541
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 150,440,224 bp
  • C to T, chromosome 1 at 150,609,825 bp
  • G to T, chromosome 2 at 17,434,972 bp
  • T to A, chromosome 2 at 76,732,358 bp
  • T to A, chromosome 2 at 111,873,562 bp
  • T to C, chromosome 2 at 127,123,675 bp
  • T to A, chromosome 3 at 89,443,159 bp
  • A to T, chromosome 3 at 89,831,790 bp
  • A to T, chromosome 3 at 135,408,840 bp
  • G to T, chromosome 4 at 32,562,681 bp
  • A to G, chromosome 4 at 43,705,990 bp
  • T to A, chromosome 4 at 96,769,668 bp
  • C to G, chromosome 6 at 136,329,557 bp
  • T to C, chromosome 7 at 4,757,926 bp
  • T to G, chromosome 7 at 27,748,515 bp
  • A to G, chromosome 8 at 23,180,664 bp
  • T to A, chromosome 9 at 105,738,236 bp
  • T to A, chromosome 9 at 107,561,415 bp
  • T to C, chromosome 10 at 13,261,846 bp
  • A to T, chromosome 10 at 83,522,785 bp
  • C to A, chromosome 10 at 86,048,026 bp
  • C to T, chromosome 10 at 130,271,067 bp
  • C to T, chromosome 11 at 16,954,280 bp
  • T to C, chromosome 11 at 30,815,246 bp
  • A to T, chromosome 11 at 74,346,914 bp
  • T to A, chromosome 14 at 48,512,170 bp
  • C to T, chromosome 15 at 66,615,785 bp
  • C to A, chromosome 15 at 84,406,510 bp
  • T to A, chromosome 15 at 100,682,980 bp
  • T to C, chromosome 19 at 5,070,209 bp
  • G to T, chromosome 19 at 31,918,062 bp
  • A to T, chromosome 19 at 32,800,141 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5491 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043052-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.