Strain Name:
C57BL/6J-MtgxR5494Btlr/Mmmh
Stock Number:
043055-MU
Citation ID:
RRID:MMRRC_043055-MU
Other Names:
R5494 (G1), C57BL/6J-MtgxR5494Btlr
Major Collection:

Gene Information

Atrn
Name: attractin
Synonyms: Mgca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11990
HGNC: HGNC:885
Homologene: 22542
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Fzr1
Name: fizzy and cell division cycle 20 related 1
Synonyms: Fyr, Cdh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56371
Homologene: 9444
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: LIP1, LKB1IP, 1200014D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71728
Homologene: 12406
Vps13c
Name: vacuolar protein sorting 13C
Synonyms: C230055H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320528
VEGA: 9
Homologene: 41188
Dctn1
Name: dynactin 1
Synonyms: Glued, p150, p150glued
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 13191
HGNC: HGNC:2711
Homologene: 3011
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 320365
Homologene: 113770
Atp2c1
Name: ATPase, Ca++-sequestering
Synonyms: 1700121J11Rik, PMR1, ATP2C1A, D930003G21Rik, SPCA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235574
Homologene: 56672
Med25
Name: mediator complex subunit 25
Synonyms: ESTM2, 2610529E18Rik, 2610034E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75613
Homologene: 12614
Scfd1
Name: Sec1 family domain containing 1
Synonyms: 3110021P21Rik, STXBP1L2, RA410
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76983
VEGA: 12
Homologene: 5650
Mgat4a
Name: mannoside acetylglucosaminyltransferase 4, isoenzyme A
Synonyms: GnT-IVa, 9530018I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269181
HGNC: HGNC:7047
Homologene: 8153
Frem1
Name: Fras1 related extracellular matrix protein 1
Synonyms: heb, eyes2, eyem02Jus, crf11, QBRICK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329872
Homologene: 27049
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Rbms2
Name: RNA binding motif, single stranded interacting protein 2
Synonyms: Scr3, 2610315E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56516
VEGA: 10
HGNC: HGNC:9909
Homologene: 37706
Igf2r
Name: insulin-like growth factor 2 receptor
Synonyms: CD222, IGF-II/CI-MPR, CI-MPR, mannose-6-phosphate receptor, cation independent, Mpr300, M6P/IGF2R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16004
HGNC: HGNC:5467
Homologene: 676
Gemin5
Name: gem nuclear organelle associated protein 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216766
Homologene: 9155
Robo4
Name: roundabout guidance receptor 4
Synonyms: Magic roundabout, 1200012D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Gucy1b1
Name: guanylate cyclase 1, soluble, beta 1
Synonyms: Gucy1b3, beta 1 sGC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 54195
HGNC: HGNC:4687
Homologene: 664
Hc
Name: hemolytic complement
Synonyms: C5, C5a, Hfib2, He
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15139
HGNC: HGNC:1331
Homologene: 20412
Ak9
Name: adenylate kinase 9
Synonyms: Akd1, LOC215946, Gm7127, Akd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 633979
Homologene: 67934
Oas3
Name: 2'-5' oligoadenylate synthetase 3
Synonyms: Oasl10, 2'-5' oligoadenylate synthetase-like 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 246727
HGNC: HGNC:8088
Homologene: 4510
Golgb1
Name: golgi autoantigen, golgin subfamily b, macrogolgin 1
Synonyms: F730017E11Rik, Gm6840, Giantin, 6330407A06Rik, C130074L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224139
HGNC: HGNC:4429
Homologene: 68401
Ccdc81
Name: coiled-coil domain containing 81
Synonyms: 4921513D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70884
Homologene: 128447
Heg1
Name: heart development protein with EGF-like domains 1
Synonyms: 5530401I02Rik, 9530025L16Rik, 4632417D23Rik, LOC268884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77446
Homologene: 35276
Dgkz
Name: diacylglycerol kinase zeta
Synonyms: E130307B02Rik, mDGK[z]
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 104418
HGNC: HGNC:2857
Homologene: 37831
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: brachyphalangy, Bph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Plch2
Name: phospholipase C, eta 2
Synonyms: Plcl4, A930027K05Rik, PLCeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269615
Homologene: 85172
Eif4a3l1
Name: eukaryotic translation initiation factor 4A3 like 1
Synonyms: B020013A22Rik, Gm8994
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 668137
Trim62
Name: tripartite motif-containing 62
Synonyms: Dear1, 6330414G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67525
Homologene: 10071
Slc28a1
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 1
Synonyms: Cnt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434203
Homologene: 37902
Aldh18a1
Name: aldehyde dehydrogenase 18 family, member A1
Synonyms: Pycs, 2810433K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56454
HGNC: HGNC:9722
Homologene: 2142
Shank3
Name: SH3 and multiple ankyrin repeat domains 3
Synonyms: ProSAP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 58234
Homologene: 75163
Pcdhb17
Name: protocadherin beta 17
Synonyms: Pcdhb16, PcdhbQ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93888
Homologene: 81881
Tspoap1
Name: TSPO associated protein 1
Synonyms: Bzrap1, peripheral
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207777
Homologene: 37961
Olfr655
Name: olfactory receptor 655
Synonyms: GA_x6K02T2PBJ9-7224628-7223702, MOR38-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258817
Homologene: 17403
Krt1
Name: keratin 1
Synonyms: Krt-2.1, Krt2-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Clvs1
Name: clavesin 1
Synonyms: 4933402J24Rik, Rlbp1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74438
Homologene: 65267
Tas2r102
Name: taste receptor, type 2, member 102
Synonyms: mt2r51, Tas2r2, mGR02, STC 9-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387339
Homologene: 41543
Olfr1140
Name: olfactory receptor 1140
Synonyms: GA_x6K02T2Q125-49250025-49250960, MOR177-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258635
Homologene: 74081
Thg1l
Name: tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Synonyms: 5730409G07Rik, 1700121M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66628
Homologene: 5959
Asb18
Name: ankyrin repeat and SOCS box-containing 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 208372
Homologene: 16382
Polm
Name: polymerase (DNA directed), mu
Synonyms: B230309I03Rik, Tdt-N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 54125
HGNC: HGNC:9185
Homologene: 41170
Cep112
Name: centrosomal protein 112
Synonyms: Ccdc46, 1700001M19Rik, 1700029K01Rik, Macoco, 8430407H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76380
Homologene: 44915
Ddn
Name: dendrin
Synonyms: LOC328602
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13199
VEGA: 15
Homologene: 12816
Zfp703
Name: zinc finger protein 703
Synonyms: End2, Csmn1, 1110032O19Rik, Zeppo1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 353310
Homologene: 81391
Il27
Name: interleukin 27
Synonyms: IL-27, p28, IL-27p28, Il30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 246779
Homologene: 17087
Rbpjl
Name: recombination signal binding protein for immunoglobulin kappa J region-like
Synonyms: RBP-J kappa-like, Rbpsuhl, RBP-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19668
Homologene: 7512
Hils1
Name: histone H1-like protein in spermatids 1
Synonyms: TISP64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 54388
Homologene: 56818
Gjb5
Name: gap junction protein, beta 5
Synonyms: Cnx31.1, connexin 31.1, Cx31.1, Gjb-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14622
HGNC: HGNC:4287
Homologene: 3858
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 37,454,817 bp
  • C to T, chromosome 1 at 75,536,264 bp
  • T to C, chromosome 1 at 89,954,399 bp
  • A to T, chromosome 2 at 35,003,539 bp
  • T to C, chromosome 2 at 87,746,606 bp
  • A to T, chromosome 2 at 91,941,049 bp
  • T to C, chromosome 2 at 131,023,075 bp
  • GCC to GC, chromosome 2 at 164,414,410 bp
  • T to A, chromosome 3 at 82,039,876 bp
  • C to A, chromosome 4 at 9,350,869 bp
  • T to C, chromosome 4 at 82,940,753 bp
  • C to T, chromosome 4 at 127,355,554 bp
  • A to T, chromosome 4 at 128,885,206 bp
  • T to C, chromosome 4 at 154,991,122 bp
  • A to G, chromosome 5 at 120,761,644 bp
  • A to T, chromosome 5 at 150,390,667 bp
  • G to T, chromosome 6 at 83,182,564 bp
  • T to C, chromosome 6 at 132,763,143 bp
  • C to G, chromosome 6 at 136,329,557 bp
  • C to T, chromosome 7 at 44,885,801 bp
  • G to A, chromosome 7 at 81,168,039 bp
  • A to G, chromosome 7 at 89,877,573 bp
  • C to A, chromosome 7 at 104,596,725 bp
  • A to G, chromosome 7 at 126,592,928 bp
  • C to T, chromosome 8 at 26,979,205 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • G to A, chromosome 9 at 67,948,146 bp
  • A to T, chromosome 9 at 105,521,024 bp
  • A to G, chromosome 10 at 41,347,169 bp
  • C to T, chromosome 10 at 81,371,344 bp
  • G to T, chromosome 10 at 128,137,691 bp
  • G to A, chromosome 11 at 5,837,555 bp
  • G to A, chromosome 11 at 45,954,109 bp
  • T to A, chromosome 11 at 58,130,700 bp
  • C to T, chromosome 11 at 87,775,205 bp
  • T to C, chromosome 11 at 94,968,017 bp
  • A to G, chromosome 11 at 108,664,605 bp
  • A to G, chromosome 12 at 8,011,762 bp
  • A to G, chromosome 12 at 51,396,739 bp
  • A to T, chromosome 13 at 15,725,982 bp
  • C to T, chromosome 15 at 38,019,281 bp
  • C to T, chromosome 15 at 89,548,238 bp
  • C to A, chromosome 15 at 98,807,003 bp
  • C to T, chromosome 15 at 101,850,714 bp
  • A to G, chromosome 16 at 33,725,434 bp
  • T to A, chromosome 16 at 36,928,683 bp
  • A to T, chromosome 17 at 12,693,145 bp
  • T to C, chromosome 18 at 37,487,247 bp
  • C to T, chromosome 19 at 40,551,290 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5494 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043055-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.