Strain Name:
C57BL/6J-MtgxR5496Btlr/Mmmh
Stock Number:
043057-MU
Citation ID:
RRID:MMRRC_043057-MU
Other Names:
R5496 (G1), C57BL/6J-MtgxR5496Btlr
Major Collection:

Gene Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFATp, NFAT1-D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Cyb561
Name: cytochrome b-561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13056
HGNC: HGNC:2571
Homologene: 37552
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Carmil1
Name: capping protein regulator and myosin 1 linker 1
Synonyms: 1110037D04Rik, Lrrc16, Carmil, Lrrc16a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68732
Homologene: 9757
Ano6
Name: anoctamin 6
Synonyms: 2900059G15Rik, F730003B03Rik, Tmem16f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105722
VEGA: 15
Homologene: 27888
Ints1
Name: integrator complex subunit 1
Synonyms: 1110015K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 68510
Homologene: 53111
Ebag9
Name: estrogen receptor-binding fragment-associated gene 9
Synonyms: Rcas1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 55960
VEGA: 15
HGNC: HGNC:3123
Homologene: 3107
Nme7
Name: NME/NM23 family member 7
Synonyms: Nm23-M7, nucleoside-diphosphate kinase, D530024H21Rik, non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 171567
Homologene: 8344
Iqgap2
Name: IQ motif containing GTPase activating protein 2
Synonyms: 4933417J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544963
VEGA: 13
HGNC: HGNC:6111
Homologene: 101543
Smurf1
Name: SMAD specific E3 ubiquitin protein ligase 1
Synonyms: 4930431E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75788
Homologene: 10712
Unc13d
Name: unc-13 homolog D
Synonyms: Munc13-4, 2610108D09Rik, Jinx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70450
Homologene: 26714
Rif1
Name: replication timing regulatory factor 1
Synonyms: 5730435J01Rik, 6530403D07Rik, D2Ertd145e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 51869
Homologene: 41231
Eps15l1
Name: epidermal growth factor receptor pathway substrate 15-like 1
Synonyms: Eps15R, 9830147J04Rik, Eps15-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 13859
Homologene: 31881
Trhr
Name: thyrotropin releasing hormone receptor
Synonyms: TRH-R1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22045
VEGA: 15
Homologene: 20707
Pan3
Name: PAN3 poly(A) specific ribonuclease subunit
Synonyms: 2700050F09Rik, A430027N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72587
Homologene: 6372
Dnah7a
Name: dynein, axonemal, heavy chain 7A
Synonyms: LOC381341, Dnahc7, Dnahc7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 627872
Homologene: 41287
Cbln1
Name: cerebellin 1 precursor protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12404
HGNC: HGNC:1543
Homologene: 74520
Gm3604
Name: predicted gene 3604
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100041979
Homologene: 136271
Timp2
Name: tissue inhibitor of metalloproteinase 2
Synonyms: TIMP-2, Timp-2, D11Bwg1104e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21858
Homologene: 2444
Il22
Name: interleukin 22
Synonyms: IL-TIF, IL-22, Iltif
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 50929
Homologene: 9669
Zfp12
Name: zinc finger protein 12
Synonyms: Krox-7, Zfp-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231866
Homologene: 65328
Stau2
Name: staufen double-stranded RNA binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 29819
Homologene: 8666
Bicra
Name: BRD4 interacting chromatin remodeling complex associated protein
Synonyms: Gltscr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243842
HGNC: HGNC:4332
Homologene: 9250
Gak
Name: cyclin G associated kinase
Synonyms: D130045N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231580
HGNC: HGNC:4113
Homologene: 3846
Kif18b
Name: kinesin family member 18B
Synonyms: N-8 kinesin, 3000004C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70218
Homologene: 15214
Zic5
Name: zinc finger protein of the cerebellum 5
Synonyms: odd-paired related, Opr, 1700049L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 65100
Homologene: 11301
Egln3
Name: egl-9 family hypoxia-inducible factor 3
Synonyms: SM-20, 2610021G09Rik, Phd3, Hif-p4h-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 112407
Homologene: 32531
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Rfx8
Name: regulatory factor X 8
Synonyms: 4933400N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 619289
Homologene: 82436
Usp2
Name: ubiquitin specific peptidase 2
Synonyms: ubp41, B930035K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 53376
Homologene: 3098
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Dbr, bls, Aim-1, Aim1, dominant brown, blanc-sale, Matp, Oca4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22293
Homologene: 9412
Atmin
Name: ATM interactor
Synonyms: Asciz, gpg6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234776
Homologene: 35321
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Gpn1
Name: GPN-loop GTPase 1
Synonyms: 2410004J02Rik, Xab1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74254
Homologene: 5257
Eaf2
Name: ELL associated factor 2
Synonyms: Traits, Festa, U19, FESTA-S, FESTA-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106389
Homologene: 10208
Cdt1
Name: chromatin licensing and DNA replication factor 1
Synonyms: 2610318F11Rik, Ris2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67177
Homologene: 32650
Kcnn3
Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3
Synonyms: small conductance calcium-activated potassium channel 3, SK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 140493
HGNC: HGNC:6292
Homologene: 20516
Kif5c
Name: kinesin family member 5C
Synonyms: Khc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16574
HGNC: HGNC:6325
Homologene: 56234
Uspl1
Name: ubiquitin specific peptidase like 1
Synonyms: E430026A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231915
Homologene: 4235
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22635
Homologene: 124417
Cdh7
Name: cadherin 7, type 2
Synonyms: CDH7L1, 9330156F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241201
HGNC: HGNC:1766
Homologene: 68391
Olfr1097
Name: olfactory receptor 1097
Synonyms: GA_x6K02T2Q125-48376288-48375341, MOR206-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258840
Homologene: 37005
Psma1
Name: proteasome subunit alpha 1
Synonyms: C2, Pros-30, HC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26440
HGNC: HGNC:9530
Homologene: 2080
Olfr116
Name: olfactory receptor 116
Synonyms: MOR218-2, GA_x6K02T2PSCP-2084102-2083137
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258625
Dnm3
Name: dynamin 3
Synonyms: B230343F03Rik, 9630020E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 103967
Homologene: 22906
Mthfsd
Name: methenyltetrahydrofolate synthetase domain containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234814
Homologene: 11245
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 77827
Homologene: 15880
Adam10
Name: a disintegrin and metallopeptidase domain 10
Synonyms: kuz, kuzbanian, 1700031C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11487
HGNC: HGNC:188
Homologene: 865
Prss39
Name: protease, serine 39
Synonyms: Tesp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21755
Homologene: 84781
Csf2rb
Name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Synonyms: AIC2B, common beta chain, CDw131, beta c, Bc, Il5rb, Il3r, Il3rb1, Csf2rb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 12983
VEGA: 15
HGNC: HGNC:2436
Homologene: 339
Gmcl1
Name: germ cell-less, spermatogenesis associated 1
Synonyms: mglc-1, 2810049L19Rik, Gcl, Btbd13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23885
Homologene: 8021
Pde6a
Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
Synonyms: Pdea
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225600
HGNC: HGNC:8785
Homologene: 380
Olfr653
Name: olfactory receptor 653
Synonyms: GA_x6K02T2PBJ9-7206970-7207923, MOR33-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57250
Homologene: 115518
Cyp3a16
Name: cytochrome P450, family 3, subfamily a, polypeptide 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13114
HGNC: HGNC:2638
Homologene: 133568
Niban3
Name: niban apoptosis regulator 3
Synonyms: Bcnp1, Fam129c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100037278
Homologene: 45462
Abcb1a
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1A
Synonyms: mdr-3, Mdr1a, P-gp, P-glycoprotein, Pgy-3, Pgy3, multiple drug resistant 1a, MDR3, Pgp, Evi32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18671
HGNC: HGNC:40
Homologene: 55496
Dyrk2
Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
Synonyms: 1810038L18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69181
VEGA: 10
HGNC: HGNC:3093
Homologene: 48437
Tlr5
Name: toll-like receptor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 53791
Homologene: 20698
H2-M11
Name: histocompatibility 2, M region locus 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224754
Homologene: 52241
Spink10
Name: serine peptidase inhibitor, Kazal type 10
Synonyms: A230091H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328971
Homologene: 131225
Zfp850
Name: zinc finger protein 850
Synonyms: C130069I09Rik, Gm4636
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043772
Glra1
Name: glycine receptor, alpha 1 subunit
Synonyms: ot, oscillator, B230397M16Rik, nmf11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14654
HGNC: HGNC:4326
Homologene: 20083
Leprot
Name: leptin receptor overlapping transcript
Synonyms: OB-RGRP, Obrgrp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230514
Homologene: 10582
Glrx2
Name: glutaredoxin 2 (thioltransferase)
Synonyms: 1700010P22Rik, Grx2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69367
Homologene: 41098
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 24056
Homologene: 23450
Diras2
Name: DIRAS family, GTP-binding RAS-like 2
Synonyms: 2900052J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68203
VEGA: 13
Homologene: 56777
Gm17108
Name: predicted gene 17108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Cfap91
Name: cilia and flagella associated protein 91
Synonyms: Spata26, 4932425I24Rik, Maats1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 320214
Homologene: 33470
Ighv1-55
Name: immunoglobulin heavy variable 1-55
Synonyms: Gm16792
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 780932
Ccl26
Name: chemokine (C-C motif) ligand 26
Synonyms: eotaxin-3, Ccl26l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 541307
Homologene: 86746
Gm8212
Name: predicted gene 8212
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 666647
Homologene: 128452
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 16,390,021 bp
  • T to C, chromosome 1 at 34,500,261 bp
  • T to C, chromosome 1 at 39,670,347 bp
  • T to G, chromosome 1 at 53,457,768 bp
  • T to A, chromosome 1 at 110,048,917 bp
  • T to A, chromosome 1 at 143,745,207 bp
  • C to T, chromosome 1 at 161,982,674 bp
  • T to A, chromosome 1 at 164,328,251 bp
  • T to C, chromosome 1 at 182,973,632 bp
  • T to C, chromosome 2 at 40,927,973 bp
  • C to G, chromosome 2 at 49,730,190 bp
  • T to C, chromosome 2 at 52,098,916 bp
  • A to G, chromosome 2 at 79,288,218 bp
  • G to T, chromosome 2 at 86,890,314 bp
  • G to C, chromosome 2 at 86,890,315 bp
  • G to A, chromosome 2 at 168,536,278 bp
  • C to T, chromosome 3 at 89,609,490 bp
  • T to A, chromosome 4 at 101,657,896 bp
  • G to A, chromosome 5 at 8,674,818 bp
  • T to A, chromosome 5 at 31,509,396 bp
  • A to G, chromosome 5 at 108,576,617 bp
  • A to G, chromosome 5 at 135,563,363 bp
  • A to G, chromosome 5 at 137,436,345 bp
  • G to A, chromosome 5 at 139,755,198 bp
  • C to A, chromosome 5 at 143,244,795 bp
  • C to A, chromosome 5 at 144,882,593 bp
  • T to A, chromosome 5 at 145,467,531 bp
  • G to A, chromosome 5 at 147,527,128 bp
  • A to G, chromosome 5 at 149,209,779 bp
  • A to G, chromosome 6 at 48,406,356 bp
  • G to T, chromosome 6 at 86,697,525 bp
  • C to T, chromosome 7 at 15,987,841 bp
  • A to C, chromosome 7 at 28,007,346 bp
  • G to C, chromosome 7 at 104,580,494 bp
  • A to G, chromosome 7 at 114,274,407 bp
  • G to A, chromosome 8 at 71,605,075 bp
  • A to G, chromosome 8 at 72,382,775 bp
  • A to G, chromosome 8 at 87,471,696 bp
  • A to G, chromosome 8 at 116,957,172 bp
  • A to T, chromosome 8 at 121,098,814 bp
  • C to T, chromosome 8 at 122,570,500 bp
  • T to C, chromosome 9 at 44,085,208 bp
  • T to G, chromosome 9 at 70,722,739 bp
  • A to G, chromosome 9 at 79,602,185 bp
  • T to C, chromosome 10 at 116,507,121 bp
  • T to G, chromosome 10 at 118,205,097 bp
  • T to C, chromosome 10 at 118,860,051 bp
  • T to C, chromosome 11 at 55,527,415 bp
  • A to T, chromosome 11 at 102,913,742 bp
  • A to G, chromosome 11 at 105,937,719 bp
  • A to G, chromosome 11 at 116,066,708 bp
  • T to G, chromosome 11 at 118,303,881 bp
  • A to G, chromosome 12 at 53,140,653 bp
  • A to T, chromosome 12 at 54,203,324 bp
  • C to G, chromosome 12 at 115,208,520 bp
  • G to A, chromosome 13 at 24,155,450 bp
  • C to T, chromosome 13 at 52,507,750 bp
  • A to T, chromosome 13 at 62,371,579 bp
  • A to G, chromosome 13 at 69,612,080 bp
  • A to G, chromosome 13 at 95,630,053 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • A to T, chromosome 14 at 44,201,157 bp
  • G to A, chromosome 14 at 122,459,343 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • G to A, chromosome 15 at 44,197,536 bp
  • T to G, chromosome 15 at 44,640,420 bp
  • A to G, chromosome 15 at 78,340,561 bp
  • T to A, chromosome 15 at 95,967,614 bp
  • T to C, chromosome 16 at 36,808,076 bp
  • A to G, chromosome 16 at 38,321,493 bp
  • T to C, chromosome 17 at 36,547,979 bp
  • T to C, chromosome 17 at 37,624,578 bp
  • A to T, chromosome 18 at 61,253,665 bp
  • T to C, chromosome 18 at 62,653,467 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5496 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043057-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.