Strain Name:
C57BL/6J-MtgxR5498Btlr/Mmmh
Stock Number:
043059-MU
Citation ID:
RRID:MMRRC_043059-MU
Other Names:
R5498 (G1), C57BL/6J-MtgxR5498Btlr
Major Collection:

Strain Information

Pias3
Name: protein inhibitor of activated STAT 3
Synonyms: Pias3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229615
Homologene: 4447
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Resf1
Name: retroelement silencing factor 1
Synonyms: GET, 2810474O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67246
Homologene: 19251
Cnot1
Name: CCR4-NOT transcription complex, subunit 1
Synonyms: 6030411K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234594
HGNC: HGNC:7877
Homologene: 9453
Bub1
Name: BUB1, mitotic checkpoint serine/threonine kinase
Synonyms: Bub1a, D2Xrf87
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12235
HGNC: HGNC:1148
Homologene: 37910
Rnpc3
Name: RNA-binding region (RNP1, RRM) containing 3
Synonyms: C030014B17Rik, 2810441O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67225
Homologene: 9746
Klf10
Name: Kruppel-like factor 10
Synonyms: mGIF, Gdnfif, Egral, Tieg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21847
VEGA: 15
Homologene: 4135
Polg
Name: polymerase (DNA directed), gamma
Synonyms: Pol gamma, polymerase gamma, mitochondrial DNA polymerase gamma, mitochondrial DNA polymerase-gamma, Polga
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18975
HGNC: HGNC:9179
Homologene: 2016
Rnd2
Name: Rho family GTPase 2
Synonyms: Arhn, Rohn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11858
Homologene: 21123
Ube3b
Name: ubiquitin protein ligase E3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 117146
Homologene: 13775
Furin
Name: furin (paired basic amino acid cleaving enzyme)
Synonyms: Fur, PACE, SPC1, Pcsk3, 9130404I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18550
HGNC: HGNC:8568
Homologene: 1930
Sarnp
Name: SAP domain containing ribonucleoprotein
Synonyms: 1110005A23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66118
VEGA: 10
Homologene: 41628
Yars2
Name: tyrosyl-tRNA synthetase 2 (mitochondrial)
Synonyms: 2210023C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70120
Homologene: 7097
4930522L14Rik
Name: RIKEN cDNA 4930522L14 gene
Synonyms: Gm42152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041734
Vps51
Name: VPS51 GARP complex subunit
Synonyms: 3110057M17Rik, 1110014N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68505
HGNC: HGNC:1172
Homologene: 34675
Tlr3
Name: toll-like receptor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 142980
Homologene: 20696
Zfp287
Name: zinc finger protein 287
Synonyms: SKAT-2, B230333C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 170740
Homologene: 23244
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: bls, Dbr, Aim1, Aim-1, dominant brown, blanc-sale, Matp, Oca4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22293
Homologene: 9412
Zscan21
Name: zinc finger and SCAN domain containing 21
Synonyms: CTfin51, RU49, Zfp-38, Zfp38, Zipro1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22697
Homologene: 56530
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Fam124a
Name: family with sequence similarity 124, member A
Synonyms: EG629059
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 629059
Homologene: 86241
Wdr24
Name: WD repeat domain 24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 268933
Homologene: 6870
Cdt1
Name: chromatin licensing and DNA replication factor 1
Synonyms: 2610318F11Rik, Ris2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67177
Homologene: 32650
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: EG640524, Spnb5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 640524
Homologene: 41150
Trim45
Name: tripartite motif-containing 45
Synonyms: 4921530N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229644
Homologene: 11865
Slc16a10
Name: solute carrier family 16 (monocarboxylic acid transporters), member 10
Synonyms: PRO0813, TAT1, 2610103N14Rik, Mct10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72472
VEGA: 10
Homologene: 75089
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: Cryabp1, alphaA-CRYBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Myom2
Name: myomesin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17930
HGNC: HGNC:7614
Homologene: 2953
Acot12
Name: acyl-CoA thioesterase 12
Synonyms: 1300004O04Rik, 4930449F15Rik, Cach
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 74156
Homologene: 12540
Cdh15
Name: cadherin 15
Synonyms: Cdh14, Mcad, M cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12555
HGNC: HGNC:1754
Homologene: 3622
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228432
Homologene: 57147
Hapln2
Name: hyaluronan and proteoglycan link protein 2
Synonyms: 4930401E20Rik, Bral1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 73940
Homologene: 11045
Rusf1
Name: RUS family member 1
Synonyms: BC017158
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233913
Homologene: 11232
Phc2
Name: polyhomeotic 2
Synonyms: Mph2, D4Ertd810e, Edr2, D130050K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 54383
HGNC: HGNC:3183
Homologene: 75090
Slfn5
Name: schlafen 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327978
Homologene: 18839
Or51b4
Name: olfactory receptor family 51 subfamily B member 4
Synonyms: 5'[b]1, MOR1-3, GA_x6K02T2PBJ9-6620959-6620024, Olfr66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18367
Homologene: 88376
Or2r11
Name: olfactory receptor family 2 subfamily R member 11
Synonyms: GA_x6K02T2P3E9-5100053-5100994, MOR257-4, Olfr458
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 258436
Homologene: 133704
Sh3bp5
Name: SH3-domain binding protein 5 (BTK-associated)
Synonyms: Sab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 24056
Homologene: 23450
Ppp2r3d
Name: protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta
Synonyms: PR59, Ppp2r3, Ppp2r3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19054
Vmn1r227
Name: vomeronasal 1 receptor 227
Synonyms: V1re6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171229
Spag17os
Name: sperm associated antigen 17, opposite strand
Synonyms: B930086A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Igkv3-9
Name: immunoglobulin kappa variable 3-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 667928
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 2 at 110,697,103 bp
  • A to T, chromosome 2 at 120,076,638 bp
  • A to G, chromosome 2 at 125,360,176 bp
  • T to C, chromosome 2 at 127,814,709 bp
  • T to A, chromosome 2 at 135,976,172 bp
  • G to T, chromosome 3 at 88,023,969 bp
  • A to G, chromosome 3 at 96,702,188 bp
  • G to A, chromosome 3 at 100,103,345 bp
  • T to C, chromosome 3 at 100,925,141 bp
  • A to G, chromosome 3 at 113,611,207 bp
  • C to A, chromosome 4 at 128,708,994 bp
  • A to T, chromosome 4 at 148,540,364 bp
  • T to A, chromosome 5 at 109,737,547 bp
  • T to C, chromosome 5 at 114,418,574 bp
  • T to C, chromosome 5 at 138,133,260 bp
  • C to T, chromosome 6 at 42,460,294 bp
  • A to G, chromosome 6 at 70,588,743 bp
  • A to G, chromosome 6 at 149,328,240 bp
  • A to G, chromosome 7 at 79,454,670 bp
  • A to T, chromosome 7 at 80,391,794 bp
  • T to G, chromosome 7 at 103,881,632 bp
  • C to T, chromosome 7 at 128,276,196 bp
  • G to A, chromosome 8 at 15,129,142 bp
  • C to T, chromosome 8 at 45,398,814 bp
  • A to T, chromosome 8 at 95,757,355 bp
  • C to T, chromosome 8 at 122,570,500 bp
  • G to T, chromosome 8 at 122,865,178 bp
  • T to C, chromosome 9 at 124,439,123 bp
  • C to T, chromosome 10 at 40,037,327 bp
  • T to A, chromosome 10 at 128,853,194 bp
  • T to C, chromosome 11 at 62,728,505 bp
  • A to T, chromosome 11 at 82,957,147 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • A to T, chromosome 13 at 42,123,158 bp
  • A to T, chromosome 13 at 55,213,302 bp
  • T to C, chromosome 13 at 91,781,233 bp
  • C to A, chromosome 14 at 31,377,495 bp
  • A to G, chromosome 14 at 62,587,409 bp
  • C to T, chromosome 15 at 11,027,785 bp
  • G to A, chromosome 15 at 38,296,039 bp
  • G to A, chromosome 15 at 76,177,454 bp
  • T to A, chromosome 16 at 16,306,523 bp
  • A to G, chromosome 17 at 20,736,043 bp
  • A to G, chromosome 17 at 25,824,561 bp
  • T to G, chromosome 19 at 6,071,033 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5498 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043059-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.