Strain Name:
C57BL/6J-MtgxR5519Btlr/Mmmh
Stock Number:
043078-MU
Citation ID:
RRID:MMRRC_043078-MU
Other Names:
R5519 (G1), C57BL/6J-MtgxR5519Btlr
Major Collection:

Strain Information

St8sia1
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Synonyms: alpha-2,8-sialyltransferase, ST8Sia I, GD3S, GD3 synthase, Siat8, Siat8a, 9330109E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20449
Homologene: 2282
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Zc3h4
Name: zinc finger CCCH-type containing 4
Synonyms: LOC330474, Bwq1, Kiaa1064-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330474
Homologene: 87150
Med30
Name: mediator complex subunit 30
Synonyms: 2510044J04Rik, 1810038N03Rik, Trap25, Thrap6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69790
VEGA: 15
Homologene: 12329
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: apollon, Bruce, A430032G04Rik, D630005A10Rik, A430040A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12211
Homologene: 7248
Pspc1
Name: paraspeckle protein 1
Synonyms: 5730470C09Rik, PSP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66645
Homologene: 10108
Tank
Name: TRAF family member-associated Nf-kappa B activator
Synonyms: I-TRAF, E430026L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21353
Homologene: 3081
Lrpprc
Name: leucine-rich PPR-motif containing
Synonyms: Lrp130, 3110001K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72416
Homologene: 32695
Mmp16
Name: matrix metallopeptidase 16
Synonyms: Membrane type 3-MMP, MT3-MMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17389
HGNC: HGNC:7162
Homologene: 55939
Zfp74
Name: zinc finger protein 74
Synonyms: Zfp66, KRAB8, 2810054M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72723
Homologene: 51861
2810021J22Rik
Name: RIKEN cDNA 2810021J22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69944
Homologene: 138400
Asph
Name: aspartate-beta-hydroxylase
Synonyms: cI-37, 2310005F16Rik, aspartyl beta-hydroxylase, calsequestrin-binding protein, Junctin, jumbug, BAH, 3110001L23Rik, junctate
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 65973
HGNC: HGNC:757
Homologene: 20910
Polr3g
Name: polymerase (RNA) III (DNA directed) polypeptide G
Synonyms: RPC32, 2310047G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67486
Homologene: 38184
Nfkb2
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
Synonyms: p52, NF kappaB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18034
VEGA: 19
HGNC: HGNC:7795
Homologene: 1873
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20265
Homologene: 21375
Rundc3a
Name: RUN domain containing 3A
Synonyms: Rpip8, Rap2ip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 51799
Homologene: 4871
Emilin2
Name: elastin microfibril interfacer 2
Synonyms: basilin, FOAP-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 246707
VEGA: 17
Homologene: 36483
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212167
Homologene: 45504
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: 6330444M21Rik, D16Ertd642e, Wdr52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 212517
Homologene: 75085
Cacna1i
Name: calcium channel, voltage-dependent, alpha 1I subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239556
HGNC: HGNC:1396
Homologene: 69331
Col9a1
Name: collagen, type IX, alpha 1
Synonyms: Col9a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12839
HGNC: HGNC:2217
Homologene: 1393
Acpp
Name: acid phosphatase, prostate
Synonyms: PAP, A030005E02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56318
HGNC: HGNC:125
Homologene: 55552
Padi2
Name: peptidyl arginine deiminase, type II
Synonyms: PAD type II, Pdi, Pdi2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18600
Homologene: 7214
Axl
Name: AXL receptor tyrosine kinase
Synonyms: Tyro7, Ark, Ufo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26362
HGNC: HGNC:905
Homologene: 7583
Zfp518b
Name: zinc finger protein 518B
Synonyms: 6820424L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100515
Homologene: 19115
Gm12790
Name: predicted gene 12790
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 115489970
Ctf2
Name: cardiotrophin 2
Synonyms: LOC244218, neuropoietin, NP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244218
Homologene: 19463
Or51a42
Name: olfactory receptor family 51 subfamily A member 42
Synonyms: GA_x6K02T2PBJ9-6793628-6792684, MOR13-2, Olfr643
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259081
Homologene: 17491
Pde11a
Name: phosphodiesterase 11A
Synonyms: A630086N24Rik, 6330414F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241489
HGNC: HGNC:8773
Homologene: 56763
Serpinb3b
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3B
Synonyms: Scca2-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 383548
Homologene: 131278
Tdpoz4
Name: TD and POZ domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 399675
Homologene: 130121
Niban3
Name: niban apoptosis regulator 3
Synonyms: Bcnp1, Fam129c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100037278
Homologene: 45462
Acadsb
Name: acyl-Coenzyme A dehydrogenase, short/branched chain
Synonyms: 1300003O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66885
HGNC: HGNC:91
Homologene: 1216
Acox1
Name: acyl-Coenzyme A oxidase 1, palmitoyl
Synonyms: Acyl-CoA oxidase, AOX, D130055E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11430
HGNC: HGNC:119
Homologene: 38299
A530084C06Rik
Name: RIKEN cDNA A530084C06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
1700067K01Rik
Name: RIKEN cDNA 1700067K01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 73453
Homologene: 85176
Ipp
Name: IAP promoted placental gene
Synonyms: D4Jhu8, Mipp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16351
HGNC: HGNC:6108
Homologene: 4309
Cybb
Name: cytochrome b-245, beta polypeptide
Synonyms: gp91phox, gp91phox, Nox2, Cgd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 13058
HGNC: HGNC:2578
Homologene: 68054
Tpm2
Name: tropomyosin 2, beta
Synonyms: Trop-2, Tpm-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22004
Homologene: 134309
Mosmo
Name: modulator of smoothened
Synonyms: BC030336
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233812
Homologene: 16600
Jakmip3
Name: janus kinase and microtubule interacting protein 3
Synonyms: 6330417G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74004
Homologene: 90425
Trdv1
Name: T cell receptor delta variable 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 627813
2510009E07Rik
Name: RIKEN cDNA 2510009E07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 72190
VEGA: 16
Homologene: 78167
Tef
Name: thyrotroph embryonic factor
Synonyms: 2310028D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 21685
Homologene: 31140
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 24,230,254 bp
  • A to G, chromosome 1 at 107,159,776 bp
  • T to C, chromosome 2 at 61,627,758 bp
  • T to A, chromosome 2 at 66,332,213 bp
  • T to A, chromosome 2 at 76,075,955 bp
  • A to T, chromosome 3 at 93,797,499 bp
  • T to C, chromosome 4 at 9,604,535 bp
  • T to C, chromosome 4 at 18,051,816 bp
  • T to G, chromosome 4 at 43,522,751 bp
  • G to A, chromosome 4 at 101,967,691 bp
  • T to C, chromosome 4 at 116,510,767 bp
  • A to G, chromosome 4 at 140,949,222 bp
  • T to A, chromosome 5 at 21,289,859 bp
  • G to A, chromosome 5 at 38,674,098 bp
  • T to C, chromosome 6 at 142,963,561 bp
  • A to T, chromosome 7 at 16,435,232 bp
  • G to A, chromosome 7 at 25,778,662 bp
  • G to T, chromosome 7 at 29,935,134 bp
  • G to A, chromosome 7 at 104,059,297 bp
  • C to T, chromosome 7 at 120,730,510 bp
  • T to A, chromosome 7 at 127,719,291 bp
  • T to C, chromosome 7 at 131,429,965 bp
  • T to C, chromosome 7 at 139,007,791 bp
  • G to A, chromosome 8 at 71,600,477 bp
  • T to C, chromosome 8 at 84,002,992 bp
  • C to A, chromosome 9 at 104,291,488 bp
  • G to A, chromosome 11 at 58,880,097 bp
  • A to T, chromosome 11 at 102,402,031 bp
  • A to G, chromosome 11 at 116,178,203 bp
  • T to C, chromosome 13 at 31,558,719 bp
  • A to T, chromosome 13 at 81,699,703 bp
  • T to A, chromosome 14 at 53,881,948 bp
  • T to C, chromosome 14 at 56,771,956 bp
  • G to T, chromosome 15 at 52,721,066 bp
  • T to C, chromosome 15 at 80,371,499 bp
  • C to T, chromosome 15 at 81,815,272 bp
  • A to G, chromosome 16 at 21,653,468 bp
  • A to T, chromosome 16 at 44,404,088 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • G to A, chromosome 17 at 71,252,935 bp
  • A to T, chromosome 17 at 74,580,178 bp
  • G to A, chromosome 17 at 84,750,518 bp
  • G to T, chromosome 19 at 46,307,567 bp
  • C to G, chromosome X at 9,450,750 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5519 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043078-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.