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Strain Name:
C57BL/6J-MtgxR5526Btlr/Mmmh
Stock Number:
043084-MU
Citation ID:
RRID:MMRRC_043084-MU
Other Names:
R5526 (G1), C57BL/6J-MtgxR5526Btlr
Major Collection:

Strain Information

Elavl3
Name: ELAV like RNA binding protein 3
Synonyms: mHuC, Huc, 2600009P04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15571
VEGA: 9
HGNC: HGNC:3314
Homologene: 31035
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Gatad2a
Name: GATA zinc finger domain containing 2A
Synonyms: 1110066C11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234366
Homologene: 9766
Phax
Name: phosphorylated adaptor for RNA export
Synonyms: p55, 4933427L19Rik, 2810055C14Rik, D18Ertd65e, phosphorylation regulated, Phax, Rnuxa
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 56698
Homologene: 10558
Fzd6
Name: frizzled class receptor 6
Synonyms: Fz6, rst
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14368
VEGA: 15
HGNC: HGNC:4044
Homologene: 2617
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328329
Homologene: 42094
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 71,292,446 bp
  • T to A, chromosome 1 at 106,030,254 bp
  • A to T, chromosome 2 at 5,911,851 bp
  • G to A, chromosome 2 at 30,285,808 bp
  • C to T, chromosome 2 at 76,980,156 bp
  • A to G, chromosome 2 at 82,258,590 bp
  • T to C, chromosome 2 at 87,457,765 bp
  • T to C, chromosome 2 at 121,305,662 bp
  • C to A, chromosome 2 at 125,365,639 bp
  • T to A, chromosome 2 at 132,861,245 bp
  • A to T, chromosome 2 at 140,008,789 bp
  • T to C, chromosome 2 at 157,323,267 bp
  • T to C, chromosome 2 at 158,432,785 bp
  • T to C, chromosome 4 at 42,972,125 bp
  • T to C, chromosome 4 at 133,601,604 bp
  • T to C, chromosome 5 at 45,733,727 bp
  • C to T, chromosome 6 at 40,612,980 bp
  • A to T, chromosome 7 at 18,349,348 bp
  • T to A, chromosome 7 at 26,382,366 bp
  • A to G, chromosome 7 at 30,580,444 bp
  • A to G, chromosome 7 at 41,872,645 bp
  • A to T, chromosome 7 at 131,041,190 bp
  • T to A, chromosome 8 at 58,472,970 bp
  • T to C, chromosome 8 at 69,935,941 bp
  • A to G, chromosome 8 at 106,873,947 bp
  • G to A, chromosome 8 at 106,917,633 bp
  • C to A, chromosome 9 at 19,713,698 bp
  • T to C, chromosome 9 at 22,036,326 bp
  • A to T, chromosome 9 at 39,719,596 bp
  • T to G, chromosome 9 at 40,150,706 bp
  • T to C, chromosome 9 at 95,871,572 bp
  • G to A, chromosome 9 at 119,521,171 bp
  • T to C, chromosome 10 at 116,918,929 bp
  • A to T, chromosome 10 at 127,555,724 bp
  • T to A, chromosome 11 at 55,269,361 bp
  • A to G, chromosome 11 at 83,296,264 bp
  • G to T, chromosome 11 at 94,704,118 bp
  • A to T, chromosome 12 at 85,269,373 bp
  • A to T, chromosome 13 at 22,524,103 bp
  • T to C, chromosome 13 at 102,754,215 bp
  • T to C, chromosome 13 at 113,367,893 bp
  • A to G, chromosome 14 at 55,864,351 bp
  • C to T, chromosome 14 at 118,631,037 bp
  • T to A, chromosome 15 at 10,326,426 bp
  • A to T, chromosome 15 at 39,031,164 bp
  • A to G, chromosome 15 at 42,512,341 bp
  • A to G, chromosome 15 at 84,791,805 bp
  • A to G, chromosome 16 at 20,737,106 bp
  • A to G, chromosome 16 at 45,660,904 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • C to A, chromosome 17 at 23,954,649 bp
  • T to C, chromosome 17 at 26,624,678 bp
  • T to A, chromosome 17 at 37,467,112 bp
  • T to A, chromosome 17 at 37,924,492 bp
  • T to G, chromosome 17 at 44,724,862 bp
  • T to G, chromosome 17 at 80,227,749 bp
  • T to A, chromosome 18 at 23,646,230 bp
  • C to T, chromosome 18 at 56,584,310 bp
  • A to T, chromosome 19 at 33,467,884 bp
  • T to A, chromosome 19 at 53,226,607 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5526 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043084-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.