Strain Name:
C57BL/6J-MtgxR5527Btlr/Mmmh
Stock Number:
043085-MU
Citation ID:
RRID:MMRRC_043085-MU
Other Names:
R5527 (G1), C57BL/6J-MtgxR5527Btlr
Major Collection:

Strain Information

Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 72895
Homologene: 12485
Gga3
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 3
Synonyms: C230037M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 260302
Homologene: 121703
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Rubcn
Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein
Synonyms: 1700021K19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100502698
Homologene: 15687
Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Cdk7
Name: cyclin dependent kinase 7
Synonyms: CRK4 PK (CDC2-related-kinase-4 protein kinase), Crk4, Cdkn7
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12572
VEGA: 13
HGNC: HGNC:1778
Homologene: 1363
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Mob2
Name: MOB kinase activator 2
Synonyms: 1110017M21Rik, 2700078K21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101513
Homologene: 12477
Naa15
Name: N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Synonyms: tubedown, Tbdn-1, mNAT1, 5730450D16Rik, Narg1, ASTBDN
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74838
Homologene: 14211
Xrcc6
Name: X-ray repair complementing defective repair in Chinese hamster cells 6
Synonyms: Ku p70, Ku70, G22p1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14375
HGNC: HGNC:4055
Homologene: 37483
Baz2a
Name: bromodomain adjacent to zinc finger domain, 2A
Synonyms: Tip5, Walp3, C030005G16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 116848
VEGA: 10
HGNC: HGNC:962
Homologene: 8393
Xrn2
Name: 5'-3' exoribonuclease 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24128
Homologene: 6927
Heatr1
Name: HEAT repeat containing 1
Synonyms: B130016L12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 217995
VEGA: 13
Homologene: 34562
Bicd1
Name: BICD cargo adaptor 1
Synonyms: B830009D06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12121
HGNC: HGNC:1049
Homologene: 37518
Zfp292
Name: zinc finger protein 292
Synonyms: Zn-16, Zn-15, Krox-10, Zfp-15, 9430062L07Rik, 5730450D02Rik, Zfp15
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30046
Homologene: 8493
Ddx47
Name: DEAD box helicase 47
Synonyms: 4930588A18Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67755
Homologene: 6092
Tpd52l2
Name: tumor protein D52-like 2
Synonyms: D54, 2810411G23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66314
Homologene: 2469
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: A930005E13Rik, LOC381562, D930005K06Rik, 1810009A16Rik, Zubr1, p600
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69116
Homologene: 10804
Il7r
Name: interleukin 7 receptor
Synonyms: IL-7 receptor alpha chain, CD127, IL-7Ralpha
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16197
VEGA: 15
HGNC: HGNC:6024
Homologene: 1646
Setd1a
Name: SET domain containing 1A
Synonyms: KMT2F
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233904
Homologene: 52251
Nrros
Name: negative regulator of reactive oxygen species
Synonyms: E430025L02Rik, Lrrc33
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224109
Homologene: 17134
Calm2
Name: calmodulin 2
Synonyms: 1500001E21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12314
HGNC: HGNC:1445
Homologene: 116117
Bach1
Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
Synonyms: 6230421P05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12013
HGNC: HGNC:935
Homologene: 916
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329002
Homologene: 7198
Thoc6
Name: THO complex 6
Synonyms: F830014G06Rik, Wdr58
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 386612
Homologene: 11533
Uxs1
Name: UDP-glucuronate decarboxylase 1
Synonyms: 1600025I13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67883
Homologene: 41609
Me2
Name: malic enzyme 2, NAD(+)-dependent, mitochondrial
Synonyms: D030040L20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107029
VEGA: 18
HGNC: HGNC:6984
Homologene: 37615
Fkbp1b
Name: FK506 binding protein 1b
Synonyms: FKBP12.6, 12.6kDa, calstabin2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14226
VEGA: 12
HGNC: HGNC:3712
Homologene: 68380
Cat
Name: catalase
Synonyms: Cs-1, Cas-1, Cas1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12359
HGNC: HGNC:1516
Homologene: 55514
Ccnt2
Name: cyclin T2
Synonyms: 2900041I18Rik, CycT2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72949
HGNC: HGNC:1600
Homologene: 14043
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Mroh4
Name: maestro heat-like repeat family member 4
Synonyms: 1700016M24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69439
Homologene: 72545
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Xpr1
Name: xenotropic and polytropic retrovirus receptor 1
Synonyms: suppressor of yeast Ga deletion, Syg1, Rmc-1, Rmc1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19775
Homologene: 134226
Nr1i3
Name: nuclear receptor subfamily 1, group I, member 3
Synonyms: CAR1, MB67, Care2, ESTM32, mCAR, CAR
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12355
HGNC: HGNC:7969
Homologene: 3759
Nlrc5
Name: NLR family, CARD domain containing 5
Synonyms: AI451557
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 434341
Homologene: 88935
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19888
Homologene: 4564
Tma16
Name: translation machinery associated 16
Synonyms: 1810029B16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66282
Homologene: 10146
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620928
Homologene: 115466
Supv3l1
Name: suppressor of var1, 3-like 1 (S. cerevisiae)
Synonyms: 6330443E10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 338359
Homologene: 2386
Usp13
Name: ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms: IsoT-3, ISOT3, 2700071E21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72607
Homologene: 68372
Dpy19l3
Name: dpy-19 like C-mannosyltransferase 3
Synonyms: 9330164H19Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233115
Homologene: 18692
Fam118a
Name: family with sequence similarity 118, member A
Synonyms: C230014M12Rik, 3110048E14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73225
VEGA: 15
HGNC: HGNC:1313
Homologene: 9911
Cenpj
Name: centromere protein J
Synonyms: 4932437H03Rik, Sas4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219103
Homologene: 10204
Lilra6
Name: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6
Synonyms: 7M1, Pira3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18726
Homologene: 134028
Ipo4
Name: importin 4
Synonyms: 8430408O15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 75751
Homologene: 5835
Fcgbp
Name: Fc fragment of IgG binding protein
Synonyms: A430096B05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 215384
Homologene: 68369
Lrrc31
Name: leucine rich repeat containing 31
Synonyms: E230002P03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 320352
Abcc1
Name: ATP-binding cassette, sub-family C member 1
Synonyms: MRP, Mdrap, Mrp1, Abcc1b, Abcc1a
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17250
HGNC: HGNC:51
Homologene: 133779
Ube2j1
Name: ubiquitin-conjugating enzyme E2J 1
Synonyms: 0710008M05Rik, Ncube, Ubc6p, 1110030I22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56228
Homologene: 41090
Lvrn
Name: laeverin
Synonyms: 4833403I15Rik, Aqpep
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 74574
VEGA: 18
Homologene: 51352
Zpld1
Name: zona pellucida like domain containing 1
Synonyms: 9430016A21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239852
Homologene: 18401
Flt4
Name: FMS-like tyrosine kinase 4
Synonyms: VEGFR3, VEGFR-3, Flt-4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14257
HGNC: HGNC:3767
Homologene: 7321
Pcare
Name: photoreceptor cilium actin regulator
Synonyms: BC027072
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 225004
VEGA: 17
Homologene: 19792
Spef1
Name: sperm flagellar 1
Synonyms: 4931426K16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70997
Homologene: 15741
Wscd2
Name: WSC domain containing 2
Synonyms: 4933413A10Rik, C530024P05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320916
Homologene: 35372
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627569
Homologene: 129606
Sbds
Name: SBDS ribosome maturation factor
Synonyms: 4733401P19Rik, Shwachman-Bodian-Diamond syndrome homolog (human)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66711
Homologene: 6438
Cplane2
Name: ciliogenesis and planar polarity effector 2
Synonyms: LOC279260, 6330545A04Rik, b2b2827Clo, b2b2804Clo, Rsg1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76166
Homologene: 12793
Ncf2
Name: neutrophil cytosolic factor 2
Synonyms: NADPH oxidase subunit (67kDa), p67phox, Ncf-2, NOXA2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17970
HGNC: HGNC:7661
Homologene: 374
Sh3tc2
Name: SH3 domain and tetratricopeptide repeats 2
Synonyms: D430044G18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225608
VEGA: 18
Homologene: 11596
Tm4sf19
Name: transmembrane 4 L six family member 19
Synonyms: EG277203
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 277203
Homologene: 64632
Lag3
Name: lymphocyte-activation gene 3
Synonyms: CD223, Ly66, LAG-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16768
HGNC: HGNC:6476
Homologene: 1719
Or7c70
Name: olfactory receptor family 7 subfamily C member 70
Synonyms: GA_x6K02T2QGN0-2962025-2962987, MOR142-2_p, MOR142-1, Olfr1356
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258305
Homologene: 71958
Efcab15
Name: EF-hand calcium binding domain 15
Synonyms: 1700023F06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 69441
Homologene: 132455
Or52m2
Name: olfactory receptor family 52 subfamily M member 2
Synonyms: GA_x6K02T2PBJ9-5333671-5332712, MOR25-2, Olfr553
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233578
Homologene: 17194
Gm12250
Name: predicted gene 12250
Synonyms: Irgb10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 631323
Homologene: 115390
Hes2
Name: hes family bHLH transcription factor 2
Synonyms: HES-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15206
Homologene: 22455
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,346,393 bp
  • T to C, chromosome 1 at 43,780,080 bp
  • T to C, chromosome 1 at 127,802,664 bp
  • T to G, chromosome 1 at 152,824,267 bp
  • T to C, chromosome 1 at 155,290,235 bp
  • A to G, chromosome 1 at 171,214,352 bp
  • A to G, chromosome 2 at 52,334,453 bp
  • A to G, chromosome 2 at 103,472,973 bp
  • T to A, chromosome 2 at 131,172,741 bp
  • A to T, chromosome 2 at 147,029,755 bp
  • G to A, chromosome 2 at 181,502,054 bp
  • T to A, chromosome 3 at 30,691,228 bp
  • A to G, chromosome 3 at 32,865,838 bp
  • T to C, chromosome 3 at 51,441,947 bp
  • C to T, chromosome 4 at 33,045,164 bp
  • A to T, chromosome 4 at 34,806,261 bp
  • T to A, chromosome 4 at 139,480,788 bp
  • C to A, chromosome 4 at 141,219,992 bp
  • T to C, chromosome 4 at 152,160,392 bp
  • A to T, chromosome 5 at 109,086,617 bp
  • C to A, chromosome 5 at 113,551,255 bp
  • T to G, chromosome 5 at 124,590,832 bp
  • T to C, chromosome 5 at 130,246,406 bp
  • C to T, chromosome 6 at 23,000,053 bp
  • C to T, chromosome 6 at 73,159,229 bp
  • A to T, chromosome 6 at 113,117,551 bp
  • T to C, chromosome 6 at 124,908,629 bp
  • C to A, chromosome 6 at 135,011,694 bp
  • T to C, chromosome 6 at 149,494,636 bp
  • T to A, chromosome 7 at 3,914,587 bp
  • T to A, chromosome 7 at 28,093,635 bp
  • A to T, chromosome 7 at 35,714,130 bp
  • T to A, chromosome 7 at 68,207,821 bp
  • T to C, chromosome 7 at 85,858,367 bp
  • A to T, chromosome 7 at 102,614,561 bp
  • C to T, chromosome 7 at 127,785,629 bp
  • A to G, chromosome 7 at 142,009,410 bp
  • A to G, chromosome 8 at 66,484,124 bp
  • A to G, chromosome 8 at 94,490,416 bp
  • C to T, chromosome 10 at 62,429,829 bp
  • C to T, chromosome 10 at 78,847,775 bp
  • T to C, chromosome 10 at 128,124,917 bp
  • T to C, chromosome 11 at 49,634,754 bp
  • A to G, chromosome 11 at 58,188,771 bp
  • G to A, chromosome 11 at 69,437,188 bp
  • A to G, chromosome 11 at 103,200,745 bp
  • G to T, chromosome 11 at 115,587,436 bp
  • T to C, chromosome 12 at 4,833,746 bp
  • T to C, chromosome 13 at 12,402,760 bp
  • T to C, chromosome 13 at 12,404,948 bp
  • C to T, chromosome 13 at 100,730,472 bp
  • G to A, chromosome 14 at 55,632,050 bp
  • T to C, chromosome 14 at 56,526,983 bp
  • A to T, chromosome 15 at 9,512,924 bp
  • A to G, chromosome 15 at 74,615,016 bp
  • G to A, chromosome 15 at 82,037,560 bp
  • A to T, chromosome 15 at 85,058,798 bp
  • T to A, chromosome 16 at 14,460,978 bp
  • T to C, chromosome 16 at 32,144,470 bp
  • T to C, chromosome 16 at 32,407,921 bp
  • T to C, chromosome 16 at 32,826,711 bp
  • C to G, chromosome 16 at 55,226,362 bp
  • T to C, chromosome 16 at 87,719,545 bp
  • A to G, chromosome 17 at 23,670,795 bp
  • A to T, chromosome 17 at 71,752,640 bp
  • A to G, chromosome 17 at 87,435,128 bp
  • G to A, chromosome 18 at 46,873,803 bp
  • A to G, chromosome 18 at 62,011,861 bp
  • A to T, chromosome 18 at 73,791,116 bp
  • A to T, chromosome 18 at 82,658,034 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5527 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043085-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.