Strain Name:
C57BL/6J-MtgxR5535Btlr/Mmmh
Stock Number:
043093-MU
Citation ID:
RRID:MMRRC_043093-MU
Other Names:
R5535 (G1), C57BL/6J-MtgxR5535Btlr
Major Collection:

Gene Information

Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52463
Homologene: 12735
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Helz
Name: helicase with zinc finger domain
Synonyms: 9630002H22Rik, 9430093I07Rik, 3110078M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78455
Homologene: 8918
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Immt
Name: inner membrane protein, mitochondrial
Synonyms: P87/89, P89, P87, HMP, 1700082C19Rik, D830041H16Rik, mitofilin, Micos60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76614
HGNC: HGNC:6047
Homologene: 38234
Ckap2l
Name: cytoskeleton associated protein 2-like
Synonyms: 2010016H04Rik, 2610318C08Rik, Radmis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70466
Homologene: 51866
Kcnh5
Name: potassium voltage-gated channel, subfamily H (eag-related), member 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238271
VEGA: 12
HGNC: HGNC:6254
Homologene: 15858
Rnf126
Name: ring finger protein 126
Synonyms: 2610010O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70294
VEGA: 10
Homologene: 27024
Atp5g3
Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228033
HGNC: HGNC:843
Homologene: 133873
Cntfr
Name: ciliary neurotrophic factor receptor
Synonyms: Cntfralpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12804
HGNC: HGNC:2170
Homologene: 9619
Flrt2
Name: fibronectin leucine rich transmembrane protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 399558
HGNC: HGNC:3761
Homologene: 8291
Plod2
Name: procollagen lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms: lysyl hydroxylase 2, LH2, Plod-2, D530025C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 26432
HGNC: HGNC:9082
Homologene: 22681
Lnpep
Name: leucyl/cystinyl aminopeptidase
Synonyms: gp160, vp165, IRAP, 4732490P18Rik, 2010309L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240028
VEGA: 17
HGNC: HGNC:6656
Homologene: 21148
Ucp3
Name: uncoupling protein 3 (mitochondrial, proton carrier)
Synonyms: UCP-3, Slc25a9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22229
Homologene: 2517
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Rdh10
Name: retinol dehydrogenase 10 (all-trans)
Synonyms: 3110069K09Rik, D1Ertd762e, 4921506A21Rik, m366Asp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98711
Homologene: 14061
Clk4
Name: CDC like kinase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 12750
Homologene: 56895
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Sdk2
Name: sidekick cell adhesion molecule 2
Synonyms: 5330435L01Rik, 4632412F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237979
Homologene: 10406
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329628
Homologene: 14377
Gm10801
Name: predicted gene 10801
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Elmo2
Name: engulfment and cell motility 2
Synonyms: 1190002F24Rik, CED-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 140579
Homologene: 44338
Unc79
Name: unc-79 homolog
Synonyms: 9030205A07Rik, Mlca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217843
Homologene: 41397
Btn2a2
Name: butyrophilin, subfamily 2, member A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238555
HGNC: HGNC:1137
Homologene: 56012
Col9a1
Name: collagen, type IX, alpha 1
Synonyms: Col9a-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12839
HGNC: HGNC:2217
Homologene: 1393
Myo15b
Name: myosin XVB
Synonyms: LOC217328, LOC380737, E330039G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217328
Parp9
Name: poly (ADP-ribose) polymerase family, member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 80285
Homologene: 12720
Agbl2
Name: ATP/GTP binding protein-like 2
Synonyms: A430081C19Rik, Ccp2, Ccp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271813
Homologene: 11715
Prex1
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
Synonyms: P-REX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 277360
Homologene: 10821
Bace2
Name: beta-site APP-cleaving enzyme 2
Synonyms: 1110059C24Rik, CEAP1, CDA13, ASP21, ALP56, BAE2, ARP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 56175
VEGA: 16
HGNC: HGNC:934
Homologene: 22696
Myo18b
Name: myosin XVIIIb
Synonyms: 4932408L24Rik, 4933411E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74376
Homologene: 53435
Efcab5
Name: EF-hand calcium binding domain 5
Synonyms: 4930563A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 319634
Homologene: 63366
Fkbpl
Name: FK506 binding protein-like
Synonyms: Ppiase-X, NG7, DIR1, WISp39, WAF-1/CIP1 stabilizing protein 39
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56299
Homologene: 10529
Prag1
Name: PEAK1 related kinase activating pseudokinase 1
Synonyms: NACK, D8Ertd82e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244418
Homologene: 18256
Acaa1b
Name: acetyl-Coenzyme A acyltransferase 1B
Synonyms: thiolase B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235674
HGNC: HGNC:82
Homologene: 91131
Amer2
Name: APC membrane recruitment 2
Synonyms: 2600011E07Rik, Amer2, Fam123a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 72125
VEGA: 14
Homologene: 45141
Olfr907
Name: olfactory receptor 907
Synonyms: GA_x6K02T2PVTD-32204729-32205661, MOR165-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258801
VEGA: 9
Homologene: 27268
Mmp25
Name: matrix metallopeptidase 25
Synonyms: Leukolysin, MT6-MMP, F730048C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240047
VEGA: 17
Homologene: 23375
Clip4
Name: CAP-GLY domain containing linker protein family, member 4
Synonyms: 1700074B05Rik, 5830409B12Rik, 4833417L20Rik, 1700024K14Rik, Rsnl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78785
VEGA: 17
Homologene: 11662
Mfhas1
Name: malignant fibrous histiocytoma amplified sequence 1
Synonyms: 2310066G09Rik, D8Ertd91e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52065
Homologene: 3114
Tmco2
Name: transmembrane and coiled-coil domains 2
Synonyms: 1700028F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69469
Homologene: 77950
Ces3a
Name: carboxylesterase 3A
Synonyms: Es-male carboxylesterase, Es31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382053
HGNC: HGNC:1865
Homologene: 84407
Hoxd13
Name: homeobox D13
Synonyms: Hox-4.8, spdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15433
HGNC: HGNC:5136
Homologene: 20147
Pcdha3
Name: protocadherin alpha 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 192163
HGNC: HGNC:8669
Homologene: 129613
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 16,131,184 bp
  • T to A, chromosome 1 at 24,230,147 bp
  • T to C, chromosome 2 at 73,909,846 bp
  • A to T, chromosome 2 at 74,668,797 bp
  • G to A, chromosome 2 at 90,810,006 bp
  • GT to GTTTTT, chromosome 2 at 98,662,499 bp
  • A to G, chromosome 2 at 129,285,842 bp
  • A to G, chromosome 2 at 165,310,212 bp
  • A to T, chromosome 2 at 166,580,273 bp
  • T to A, chromosome 3 at 38,951,391 bp
  • T to A, chromosome 4 at 41,663,216 bp
  • T to A, chromosome 4 at 121,105,993 bp
  • T to C, chromosome 5 at 112,790,042 bp
  • C to A, chromosome 6 at 71,852,784 bp
  • A to T, chromosome 7 at 100,480,666 bp
  • G to A, chromosome 8 at 16,223,330 bp
  • C to A, chromosome 8 at 35,590,269 bp
  • T to C, chromosome 8 at 36,104,014 bp
  • T to A, chromosome 8 at 105,051,564 bp
  • T to A, chromosome 9 at 38,498,998 bp
  • T to A, chromosome 9 at 92,606,569 bp
  • C to T, chromosome 9 at 119,148,406 bp
  • T to A, chromosome 10 at 14,131,022 bp
  • G to T, chromosome 10 at 62,832,907 bp
  • T to A, chromosome 10 at 79,762,699 bp
  • T to C, chromosome 11 at 51,280,319 bp
  • A to G, chromosome 11 at 77,151,921 bp
  • A to G, chromosome 11 at 107,646,120 bp
  • T to A, chromosome 11 at 113,943,158 bp
  • A to G, chromosome 11 at 115,881,301 bp
  • A to T, chromosome 12 at 51,802,326 bp
  • A to G, chromosome 12 at 75,130,907 bp
  • A to G, chromosome 12 at 95,780,426 bp
  • A to T, chromosome 12 at 103,169,703 bp
  • T to C, chromosome 13 at 23,478,275 bp
  • T to G, chromosome 13 at 96,495,497 bp
  • AAGGAGGAGGAGGAG to AAGGAGGAGGAG, chromosome 14 at 60,378,853 bp
  • A to G, chromosome 16 at 35,956,825 bp
  • A to T, chromosome 16 at 97,413,425 bp
  • T to G, chromosome 17 at 17,538,694 bp
  • A to G, chromosome 17 at 23,644,760 bp
  • G to A, chromosome 17 at 34,645,329 bp
  • A to G, chromosome 17 at 71,831,262 bp
  • G to T, chromosome 18 at 36,947,936 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5535 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043093-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.