Strain Name:
C57BL/6J-MtgxR5539Btlr/Mmmh
Stock Number:
043097-MU
Citation ID:
RRID:MMRRC_043097-MU
Other Names:
R5539 (G1), C57BL/6J-MtgxR5539Btlr
Major Collection:

Gene Information

Dnajc3
Name: DnaJ heat shock protein family (Hsp40) member C3
Synonyms: p58IPK, mp58, Prkri, Dnajc3, Dnajc3a, Dnajc3b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100037258
VEGA: 14
HGNC: HGNC:9439
Homologene: 2486
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Slc12a5
Name: solute carrier family 12, member 5
Synonyms: KCC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57138
Homologene: 10665
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75415
Homologene: 23089
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Med30
Name: mediator complex subunit 30
Synonyms: 2510044J04Rik, 1810038N03Rik, Trap25, Thrap6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69790
VEGA: 15
Homologene: 12329
Pan2
Name: PAN2 poly(A) specific ribonuclease subunit
Synonyms: 1200014O24Rik, Usp52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103135
VEGA: 10
Homologene: 5918
Trip11
Name: thyroid hormone receptor interactor 11
Synonyms: 3110031G15Rik, 2610511G22Rik, 6030460N08Rik, TRIP230, GMAP-210
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 109181
Homologene: 20897
Prpf8
Name: pre-mRNA processing factor 8
Synonyms: DBF3/PRP8, Prp8, D11Bwg0410e, Sfprp8l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192159
Homologene: 4706
Rtf1
Name: RTF1, Paf1/RNA polymerase II complex component
Synonyms: 6530416A09Rik, 2900005O08Rik, Gtl7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76246
Homologene: 133868
Trim23
Name: tripartite motif-containing 23
Synonyms: Arfd1, 6330516O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 81003
HGNC: HGNC:660
Homologene: 1251
Aldh4a1
Name: aldehyde dehydrogenase 4 family, member A1
Synonyms: Ahd-1, Ssdh1, ALDH4, A930035F14Rik, Ahd1, P5CDH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 212647
HGNC: HGNC:406
Homologene: 6081
Kri1
Name: KRI1 homolog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215194
Homologene: 133742
Ccdc141
Name: coiled-coil domain containing 141
Synonyms: ENSMUSG00000075261, CAMDI, 2610301F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 545428
Homologene: 52149
Gabrr3
Name: gamma-aminobutyric acid (GABA) receptor, rho 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 328699
VEGA: 16
Homologene: 26716
Pygo1
Name: pygopus 1
Synonyms: 2600014C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72135
Homologene: 41050
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68795
Homologene: 52092
Zfp951
Name: zinc finger protein 951
Synonyms: C230055K05Rik, Gm38611
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 626391
Homologene: 134630
Raf1
Name: v-raf-leukemia viral oncogene 1
Synonyms: Raf-1, v-Raf, sarcoma 3611 oncogene, 6430402F14Rik, c-Raf, Craf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110157
HGNC: HGNC:9829
Homologene: 48145
Pcdh12
Name: protocadherin 12
Synonyms: VE-cadherin-2, vascular endothelial cadherin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 53601
HGNC: HGNC:8657
Homologene: 9574
Nr4a3
Name: nuclear receptor subfamily 4, group A, member 3
Synonyms: TEC, NOR-1, Nor1, MINOR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18124
HGNC: HGNC:7982
Homologene: 5074
Spata31
Name: spermatogenesis associated 31
Synonyms: 4930458L03Rik, Spata31a, Fam75a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78124
Homologene: 84621
Abca4
Name: ATP-binding cassette, sub-family A (ABC1), member 4
Synonyms: Rim protein, RmP, Abc10, D430003I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11304
HGNC: HGNC:34
Homologene: 298
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: LOC382552, 2810003K23Rik, Dnahcl1, Dnahc17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Slc35b4
Name: solute carrier family 35, member B4
Synonyms: 4930474D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58246
Homologene: 5799
Ccdc175
Name: coiled-coil domain containing 175
Synonyms: 4930403N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73936
VEGA: 12
Homologene: 79144
Gm5422
Name: predicted pseudogene 5422
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432448
VEGA: 10
Col5a2
Name: collagen, type V, alpha 2
Synonyms: 1110014L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12832
HGNC: HGNC:2210
Homologene: 20119
Flg2
Name: filaggrin family member 2
Synonyms: EG229574
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229574
Homologene: 134146
Mybpc2
Name: myosin binding protein C, fast-type
Synonyms: Fast-type C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233199
HGNC: HGNC:7550
Homologene: 3331
Ces1d
Name: carboxylesterase 1D
Synonyms: TGH, Ces3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104158
HGNC: HGNC:1863
Homologene: 35606
Nxpe3
Name: neurexophilin and PC-esterase domain family, member 3
Synonyms: LOC208684, LOC385658, Fam55c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 385658
VEGA: 16
Homologene: 17030
Tor2a
Name: torsin family 2, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 30933
Homologene: 25260
Lcp1
Name: lymphocyte cytosolic protein 1
Synonyms: L-fimbrin, Pls2, D14Ertd310e, L-plastin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18826
HGNC: HGNC:6528
Homologene: 80174
Gm10717
Name: predicted gene 10717
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Prss40
Name: protease, serine 40
Synonyms: Tesp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21756
Homologene: 69043
Trmt10c
Name: tRNA methyltransferase 10C, mitochondrial RNase P subunit
Synonyms: 1300018J16Rik, D16Ertd454e, Rg9mtd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 52575
Homologene: 9858
Olfr24
Name: olfactory receptor 24
Synonyms: MTPCR51, MOR132-1, GA_x6K02T2PVTD-12498544-12497603
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18322
HGNC: HGNC:8220
Homologene: 64896
Olfr482
Name: olfactory receptor 482
Synonyms: GA_x6K02T2PBJ9-10424354-10423383, MOR204-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258728
Homologene: 133604
Fndc5
Name: fibronectin type III domain containing 5
Synonyms: PeP, 1500001L03Rik, Pxp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 384061
Homologene: 17812
Cybb
Name: cytochrome b-245, beta polypeptide
Synonyms: gp91phox, gp91phox, Nox2, Cgd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 13058
HGNC: HGNC:2578
Homologene: 68054
Olfr934
Name: olfactory receptor 934
Synonyms: GA_x6K02T2PVTD-32678895-32677963, MOR224-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258434
VEGA: 9
Homologene: 51730
Rogdi
Name: rogdi homolog
Synonyms: 0610011C19Rik, Lzf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66049
Homologene: 11605
Ntf5
Name: neurotrophin 5
Synonyms: NT-4, NT4/5, NT4, neurotrophin-4, Ntf-5, Ntf4, 2900040K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78405
HGNC: HGNC:8024
Homologene: 4503
Prox2
Name: prospero homeobox 2
Synonyms: 1700058C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73422
Homologene: 18421
Gm9271
Name: predicted gene 9271
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668618
Homologene: 45597
CR974439.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
AC125199.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,552,679 bp
  • T to A, chromosome 1 at 45,423,706 bp
  • T to C, chromosome 2 at 32,760,660 bp
  • A to T, chromosome 2 at 70,020,533 bp
  • A to T, chromosome 2 at 77,015,093 bp
  • A to G, chromosome 2 at 119,729,924 bp
  • T to A, chromosome 2 at 164,987,206 bp
  • T to A, chromosome 3 at 93,220,446 bp
  • C to T, chromosome 3 at 98,137,582 bp
  • A to G, chromosome 3 at 122,169,908 bp
  • T to A, chromosome 4 at 48,056,525 bp
  • T to A, chromosome 4 at 129,138,721 bp
  • T to C, chromosome 4 at 139,638,522 bp
  • T to C, chromosome 4 at 143,132,694 bp
  • C to T, chromosome 5 at 104,814,846 bp
  • A to G, chromosome 6 at 34,176,802 bp
  • G to T, chromosome 6 at 115,619,356 bp
  • T to C, chromosome 7 at 27,327,724 bp
  • A to G, chromosome 7 at 39,362,884 bp
  • A to G, chromosome 7 at 44,514,893 bp
  • G to T, chromosome 7 at 45,415,930 bp
  • A to T, chromosome 7 at 108,095,226 bp
  • G to A, chromosome 8 at 93,197,762 bp
  • A to T, chromosome 9 at 3,030,438 bp
  • G to A, chromosome 9 at 18,754,838 bp
  • G to A, chromosome 9 at 21,279,372 bp
  • T to C, chromosome 9 at 38,982,277 bp
  • C to T, chromosome 9 at 72,944,779 bp
  • A to G, chromosome 10 at 31,248,650 bp
  • C to A, chromosome 10 at 128,308,133 bp
  • A to G, chromosome 11 at 75,503,638 bp
  • T to C, chromosome 11 at 118,073,660 bp
  • T to C, chromosome 12 at 72,144,813 bp
  • G to T, chromosome 12 at 85,095,064 bp
  • A to G, chromosome 12 at 101,885,127 bp
  • T to A, chromosome 13 at 64,922,969 bp
  • T to C, chromosome 13 at 104,198,033 bp
  • T to C, chromosome 14 at 75,229,298 bp
  • G to A, chromosome 14 at 118,970,747 bp
  • G to T, chromosome 15 at 52,721,066 bp
  • G to A, chromosome 16 at 5,010,009 bp
  • A to G, chromosome 16 at 55,890,671 bp
  • G to A, chromosome 16 at 56,034,961 bp
  • A to T, chromosome 16 at 59,461,395 bp
  • A to T, chromosome 16 at 88,759,029 bp
  • T to A, chromosome 17 at 37,610,755 bp
  • A to T, chromosome 18 at 6,111,932 bp
  • T to C, chromosome 18 at 38,281,744 bp
  • C to G, chromosome X at 9,450,750 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5539 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043097-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.