Strain Name:
C57BL/6J-MtgxR5550Btlr/Mmmh
Stock Number:
043107-MU
Citation ID:
RRID:MMRRC_043107-MU
Other Names:
R5550 (G1), C57BL/6J-MtgxR5550Btlr
Major Collection:

Strain Information

Dpy30
Name: dpy-30, histone methyltransferase complex regulatory subunit
Synonyms: 2810410M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66310
VEGA: 17
Homologene: 32582
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Dlgap4
Name: DLG associated protein 4
Synonyms: WBP16, SAP90/PSD-95-associated protein 4, PSD-95/SAP90 binding protein 4, DAP4, Sapap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228836
Homologene: 8935
Tnks2
Name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Synonyms: 5430432P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74493
Homologene: 11890
Trip12
Name: thyroid hormone receptor interactor 12
Synonyms: Gtl6, 1110036I07Rik, 6720416K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14897
Homologene: 44226
Mdc1
Name: mediator of DNA damage checkpoint 1
Synonyms: NFBD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240087
Homologene: 67092
Xpo5
Name: exportin 5
Synonyms: 2410004H11Rik, 2700038C24Rik, Exp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72322
VEGA: 17
Homologene: 69316
Adgrb2
Name: adhesion G protein-coupled receptor B2
Synonyms: Bai2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230775
HGNC: HGNC:944
Homologene: 1288
Gcat
Name: glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
Synonyms: Kbl, aminoacetone synthase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26912
VEGA: 15
HGNC: HGNC:4188
Homologene: 8475
Cux2
Name: cut-like homeobox 2
Synonyms: Cux-2, Cux2, ENSMUSG00000072641, Cutl2, 1700051K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13048
Homologene: 22426
Kank4
Name: KN motif and ankyrin repeat domains 4
Synonyms: Ankrd38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242553
Homologene: 18244
Tbc1d2
Name: TBC1 domain family, member 2
Synonyms: LOC381605, PARIS-1, PARIS1, A630005A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381605
Homologene: 10190
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1, G1-415-19, ferf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Nfkbid
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, delta
Synonyms: IkappaBNS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243910
Homologene: 13101
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237954
Homologene: 138824
Sntg1
Name: syntrophin, gamma 1
Synonyms: G1SYN, SYN4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71096
Homologene: 56834
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Henmt1
Name: HEN1 methyltransferase homolog 1 (Arabidopsis)
Synonyms: Hen1, 4921515J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66715
Homologene: 32638
Ces2b
Name: carboxyesterase 2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234669
HGNC: HGNC:1864
Homologene: 135851
Aatk
Name: apoptosis-associated tyrosine kinase
Synonyms: AATYK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11302
HGNC: HGNC:21
Homologene: 74861
Speg
Name: SPEG complex locus
Synonyms: BPEG, SPEGbeta, SPEGalpha, D1Bwg1450e, SPEG, Apeg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11790
Homologene: 55619
Or2ag15
Name: olfactory receptor family 2 subfamily AG member 15
Synonyms: GA_x6K02T2PBJ9-9119301-9118348, MOR283-5, Olfr697
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258592
Gbp4
Name: guanylate binding protein 4
Synonyms: Mag-2, Mpa-2, Mpa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17472
Homologene: 128731
P2ry1
Name: purinergic receptor P2Y, G-protein coupled 1
Synonyms: P2Y1, P2Y1 receptor, P2y1r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18441
HGNC: HGNC:8539
Homologene: 1926
Tdpoz4
Name: TD and POZ domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 399675
Homologene: 130121
Fastkd1
Name: FAST kinase domains 1
Synonyms: 5330408N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320720
Homologene: 36420
Bdh2
Name: 3-hydroxybutyrate dehydrogenase, type 2
Synonyms: 1810026B04Rik, Dhrs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69772
Homologene: 134268
Or6c75
Name: olfactory receptor family 6 subfamily C member 75
Synonyms: GA_x6K02T2PULF-11179777-11180721, MOR112-1, Olfr790
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258935
Homologene: 74101
Bud23
Name: BUD23, rRNA methyltransferase and ribosome maturation factor
Synonyms: 1110003N24Rik, Wbscr22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66138
Homologene: 5486
Adig
Name: adipogenin
Synonyms: SMAF1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 246747
H2bc27
Name: H2B clustered histone 27
Synonyms: 1500011O09Rik, Hist3h2ba, H2bu2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78303
Homologene: 134740
Dio3
Name: deiodinase, iodothyronine type III
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 107585
HGNC: HGNC:2885
Homologene: 1044
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 8,624,784 bp
  • A to T, chromosome 1 at 75,429,100 bp
  • A to G, chromosome 1 at 84,761,099 bp
  • A to T, chromosome 2 at 69,705,394 bp
  • A to G, chromosome 2 at 156,746,196 bp
  • A to T, chromosome 2 at 158,507,960 bp
  • T to C, chromosome 3 at 61,003,811 bp
  • A to T, chromosome 3 at 93,797,499 bp
  • A to G, chromosome 3 at 108,953,868 bp
  • A to G, chromosome 3 at 135,288,313 bp
  • G to A, chromosome 4 at 46,646,138 bp
  • A to G, chromosome 4 at 98,771,441 bp
  • G to T, chromosome 4 at 130,014,934 bp
  • C to T, chromosome 5 at 105,122,045 bp
  • G to A, chromosome 5 at 121,873,841 bp
  • A to G, chromosome 5 at 135,063,890 bp
  • T to A, chromosome 7 at 30,426,001 bp
  • T to C, chromosome 7 at 106,741,133 bp
  • A to G, chromosome 8 at 104,838,437 bp
  • A to G, chromosome 10 at 129,501,783 bp
  • A to G, chromosome 11 at 58,949,320 bp
  • T to A, chromosome 11 at 103,498,177 bp
  • T to C, chromosome 11 at 120,009,303 bp
  • A to T, chromosome 12 at 110,280,126 bp
  • T to A, chromosome 14 at 31,316,708 bp
  • G to C, chromosome 15 at 48,185,357 bp
  • G to A, chromosome 15 at 79,042,211 bp
  • G to A, chromosome 17 at 12,243,558 bp
  • A to G, chromosome 17 at 35,845,884 bp
  • T to A, chromosome 17 at 46,234,492 bp
  • A to G, chromosome 17 at 74,315,925 bp
  • T to A, chromosome 19 at 36,862,346 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5550 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043107-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.