Strain Name:
C57BL/6J-MtgxR5561Btlr/Mmmh
Stock Number:
043118-MU
Citation ID:
RRID:MMRRC_043118-MU
Other Names:
R5561 (G1), C57BL/6J-MtgxR5561Btlr
Major Collection:

Strain Information

Ing4
Name: inhibitor of growth family, member 4
Synonyms: p29ING4, D6Xrf92, D6Wsu147e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28019
Homologene: 22952
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: RA-GEF-1, Pdzgef1, CNRasGEF, nRapGEP, 5830453M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76089
Homologene: 35477
Adipor2
Name: adiponectin receptor 2
Synonyms: 1110001I14Rik, D6Ucla1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 68465
Homologene: 56119
Mllt10
Name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
Synonyms: B130021D15Rik, D630001B22Rik, Af10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17354
Homologene: 20973
Eed
Name: embryonic ectoderm development
Synonyms: l(7)5Rn, l7Rn5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13626
HGNC: HGNC:3188
Homologene: 2814
Fancc
Name: Fanconi anemia, complementation group C
Synonyms: Facc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14088
HGNC: HGNC:3584
Homologene: 109
Mdc1
Name: mediator of DNA damage checkpoint 1
Synonyms: NFBD1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240087
Homologene: 67092
Fer
Name: fer (fms/fps related) protein kinase
Synonyms: Fert2, Fert, C330004K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14158
VEGA: 17
HGNC: HGNC:3655
Homologene: 74300
Ift80
Name: intraflagellar transport 80
Synonyms: Wdr56, 4921524P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68259
Homologene: 12253
Foxi2
Name: forkhead box I2
Synonyms: B130055A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 270004
Homologene: 18838
Opn3
Name: opsin 3
Synonyms: encephalopsin, ERO, panopsin, Ecpn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13603
Homologene: 40707
Helq
Name: helicase, POLQ-like
Synonyms: D430018E21Rik, Hel308
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 191578
Homologene: 14667
Fbf1
Name: Fas (TNFRSF6) binding factor 1
Synonyms: 1110033G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217335
Homologene: 16531
Morc1
Name: microrchidia 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17450
VEGA: 16
HGNC: HGNC:7198
Homologene: 7843
Synrg
Name: synergin, gamma
Synonyms: L71-5, Ap1gbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217030
HGNC: HGNC:557
Homologene: 105680
Zdhhc12
Name: zinc finger, DHHC domain containing 12
Synonyms: 1190004A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66220
Homologene: 11934
Cops8
Name: COP9 signalosome subunit 8
Synonyms: Csn8, Sgn8, 9430009J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108679
Homologene: 4882
Palld
Name: palladin, cytoskeletal associated protein
Synonyms: 2410003B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72333
Homologene: 75052
Ppp1r12c
Name: protein phosphatase 1, regulatory subunit 12C
Synonyms: 2410197A17Rik, Mbs85
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232807
Homologene: 75071
Brd8
Name: bromodomain containing 8
Synonyms: p120, 2610007E11Rik, SMAP, 4432404P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 78656
Homologene: 41790
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 77505
Homologene: 131117
Col24a1
Name: collagen, type XXIV, alpha 1
Synonyms: 5430404K19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71355
Homologene: 65061
C8b
Name: complement component 8, beta polypeptide
Synonyms: 4930439B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110382
HGNC: HGNC:1353
Homologene: 48
Ttn
Name: titin
Synonyms: L56, 1100001C23Rik, D330041I19Rik, 2310057K23Rik, D830007G01Rik, 2310074I15Rik, 2310036G12Rik, mdm, connectin, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: Ugcgl2, A230065J02Rik, 3110027P15Rik, 1810064L21Rik, 3110001A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66435
Homologene: 56875
Hgsnat
Name: heparan-alpha-glucosaminide N-acetyltransferase
Synonyms: Tmem76, D8Ertd354e, 9430010M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52120
Homologene: 15586
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100213
Homologene: 18967
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: Cpfl8, Nesp2g, 6820443O06Rik, D12Ertd777e, nesprin-2, syne-2, dice, diminished cone electroretinogram
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Spats2l
Name: spermatogenesis associated, serine-rich 2-like
Synonyms: A230104H11Rik, 2810022L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67198
Homologene: 56711
Ephb2
Name: Eph receptor B2
Synonyms: Sek3, eteck, Nuk, Cek5, Qek5, Hek5, Drt, Erk, Tyro5, Prkm5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13844
HGNC: HGNC:3393
Homologene: 37925
Fer1l6
Name: fer-1-like 6 (C. elegans)
Synonyms: EG631797
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 631797
Homologene: 53396
Nav3
Name: neuron navigator 3
Synonyms: steerin 3, 4732483H20Rik, unc53H3, Pomfil1p, 9630020C08Rik, POMFIL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 260315
Homologene: 56688
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mass1, Gpr98, Mgr1, VLGR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: OTTMUSG00000020804, Heatr7b1, ENSMUSG00000044873
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Atxn7
Name: ataxin 7
Synonyms: A430107N12Rik, Sca7, ataxin-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 246103
VEGA: 14
Homologene: 30967
Ccdc110
Name: coiled-coil domain containing 110
Synonyms: LOC212392
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212392
Homologene: 17668
Slco3a1
Name: solute carrier organic anion transporter family, member 3a1
Synonyms: MJAM, Anr1, 5830414C08Rik, Slc21a11, OATP-D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108116
Homologene: 40862
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Or2d36
Name: olfactory receptor family 2 subfamily D member 36
Synonyms: Olfr716, MOR260-2, GA_x6K02T2PBJ9-9497411-9498355
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258597
Homologene: 121514
Stox2
Name: storkhead box 2
Synonyms: 4933409N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71069
Homologene: 18414
Vmn2r53
Name: vomeronasal 2, receptor 53
Synonyms: EG637908
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 637908
Homologene: 104040
Rpl22l1
Name: ribosomal protein L22 like 1
Synonyms: 3110001N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68028
Homologene: 134718
Dlg5
Name: discs large MAGUK scaffold protein 5
Synonyms: 4933429D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71228
HGNC: HGNC:2904
Homologene: 3486
Ceacam20
Name: CEA cell adhesion molecule 20
Synonyms: 9130012D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71601
Homologene: 19010
Atxn1
Name: ataxin 1
Synonyms: 2900016G23Rik, Atx1, Sca1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20238
VEGA: 13
Homologene: 281
Clip3
Name: CAP-GLY domain containing linker protein 3
Synonyms: 1500005P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76686
Homologene: 9176
Amn1
Name: antagonist of mitotic exit network 1
Synonyms: C730024G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232566
Homologene: 45811
Tm9sf1
Name: transmembrane 9 superfamily member 1
Synonyms: MP70, 1200014D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74140
Homologene: 4671
Lcp1
Name: lymphocyte cytosolic protein 1
Synonyms: Pls2, L-fimbrin, D14Ertd310e, L-plastin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18826
HGNC: HGNC:6528
Homologene: 80174
Trabd
Name: TraB domain containing
Synonyms: 5730502D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67976
Homologene: 36427
Ccdc202
Name: coiled-coil domain containing 202
Synonyms: Spertl, 4921530L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66732
VEGA: 14
Homologene: 41645
Ifit1bl1
Name: interferon induced protein with tetratricpeptide repeats 1B like 1
Synonyms: Gm14446
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 667373
Homologene: 78213
Lrif1
Name: ligand dependent nuclear receptor interacting factor 1
Synonyms: 2010012G17Rik, 4933421E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 321000
Homologene: 10160
Spire1
Name: spire type actin nucleation factor 1
Synonyms: Spir-1, 6030430B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 68166
VEGA: 18
Homologene: 35507
Psme1
Name: proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Synonyms: PA28a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19186
HGNC: HGNC:9568
Homologene: 4560
Dnajb12
Name: DnaJ heat shock protein family (Hsp40) member B12
Synonyms: mDj10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56709
Homologene: 9749
Gm11595
Name: predicted gene 11595
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100040276
Homologene: 124486
Hs3st5
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 5
Synonyms: D930005L05Rik, LOC382362
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 319415
Homologene: 17796
Dnase1l3
Name: deoxyribonuclease 1-like 3
Synonyms: DNasegamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13421
HGNC: HGNC:2959
Homologene: 3630
H2-DMb2
Name: histocompatibility 2, class II, locus Mb2
Synonyms: H-2Mb2, H2-M beta2, H2-Mb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15000
HGNC: HGNC:4935
Homologene: 68231
Hdhd3
Name: haloacid dehalogenase-like hydrolase domain containing 3
Synonyms: 2810435D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72748
Homologene: 11497
Rpl13
Name: ribosomal protein L13
Synonyms: L13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270106
Homologene: 5568
Rpp14
Name: ribonuclease P 14 subunit
Synonyms: 2610511E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67053
Homologene: 5113
Or12j2
Name: olfactory receptor family 12 subfamily J member 2
Synonyms: Olfr527, GA_x6K02T2PBJ9-42486061-42486978, MOR251-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257939
Homologene: 73958
Smtnl1
Name: smoothelin-like 1
Synonyms: Chasm, 1110030K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68678
Homologene: 11488
Acyp2
Name: acylphosphatase 2, muscle type
Synonyms: 2310004B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75572
HGNC: HGNC:180
Homologene: 41776
Pcdhga12
Name: protocadherin gamma subfamily A, 12
Synonyms: Pcdh13, pc2c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93724
HGNC: HGNC:8699
Homologene: 134588
Ring1
Name: ring finger protein 1
Synonyms: Ring1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19763
Homologene: 68283
Ugt1a5
Name: UDP glucuronosyltransferase 1 family, polypeptide A5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 394433
Homologene: 88621
A630095N17Rik
Name: RIKEN cDNA A630095N17 gene
Synonyms: LOC381272
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381272
Gm2666
Name: predicted gene 2666
Synonyms: Gm16182
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040213
Homologene: 86179
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 57,900,621 bp
  • T to C, chromosome 1 at 75,220,537 bp
  • T to C, chromosome 1 at 85,484,740 bp
  • T to A, chromosome 1 at 88,166,317 bp
  • GCCC to GC, chromosome 1 at 88,232,257 bp
  • T to G, chromosome 1 at 90,612,178 bp
  • C to T, chromosome 1 at 175,665,587 bp
  • T to A, chromosome 2 at 18,109,845 bp
  • A to T, chromosome 2 at 30,092,484 bp
  • T to A, chromosome 2 at 76,707,233 bp
  • C to T, chromosome 2 at 84,818,395 bp
  • T to A, chromosome 3 at 28,806,820 bp
  • T to G, chromosome 3 at 68,967,863 bp
  • A to T, chromosome 3 at 79,088,643 bp
  • A to T, chromosome 3 at 106,732,380 bp
  • T to C, chromosome 3 at 145,298,827 bp
  • C to T, chromosome 4 at 43,415,932 bp
  • T to C, chromosome 4 at 62,499,948 bp
  • T to C, chromosome 4 at 104,784,448 bp
  • C to T, chromosome 4 at 136,661,406 bp
  • G to T, chromosome 5 at 100,787,050 bp
  • G to A, chromosome 6 at 119,370,213 bp
  • C to T, chromosome 6 at 125,047,060 bp
  • G to A, chromosome 6 at 149,185,024 bp
  • A to T, chromosome 7 at 4,486,356 bp
  • A to T, chromosome 7 at 12,601,420 bp
  • A to T, chromosome 7 at 19,970,393 bp
  • A to G, chromosome 7 at 30,298,849 bp
  • A to G, chromosome 7 at 74,318,499 bp
  • G to A, chromosome 7 at 89,967,793 bp
  • G to A, chromosome 7 at 105,714,821 bp
  • A to G, chromosome 7 at 107,148,090 bp
  • A to G, chromosome 7 at 135,411,647 bp
  • C to T, chromosome 7 at 140,336,152 bp
  • A to G, chromosome 8 at 25,946,334 bp
  • T to G, chromosome 8 at 45,940,609 bp
  • T to C, chromosome 8 at 47,193,006 bp
  • G to A, chromosome 8 at 61,516,585 bp
  • C to A, chromosome 8 at 123,102,679 bp
  • C to G, chromosome 9 at 108,105,511 bp
  • T to A, chromosome 10 at 36,833,429 bp
  • GC to G, chromosome 10 at 59,892,752 bp
  • C to A, chromosome 10 at 109,716,552 bp
  • C to T, chromosome 11 at 30,506,354 bp
  • G to A, chromosome 11 at 59,036,093 bp
  • G to A, chromosome 11 at 84,002,240 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • T to C, chromosome 11 at 116,157,820 bp
  • C to T, chromosome 12 at 76,094,458 bp
  • G to T, chromosome 13 at 45,566,871 bp
  • T to C, chromosome 13 at 63,317,387 bp
  • A to G, chromosome 13 at 81,476,564 bp
  • A to G, chromosome 14 at 7,967,847 bp
  • A to G, chromosome 14 at 8,090,558 bp
  • A to T, chromosome 14 at 14,089,260 bp
  • T to A, chromosome 14 at 24,177,792 bp
  • G to A, chromosome 14 at 55,579,729 bp
  • C to T, chromosome 14 at 55,638,097 bp
  • G to A, chromosome 14 at 75,212,508 bp
  • C to A, chromosome 14 at 95,882,371 bp
  • C to T, chromosome 14 at 119,041,527 bp
  • A to G, chromosome 15 at 58,660,825 bp
  • T to C, chromosome 15 at 89,081,984 bp
  • G to T, chromosome 16 at 48,449,348 bp
  • T to C, chromosome 17 at 34,021,458 bp
  • G to T, chromosome 17 at 34,145,471 bp
  • T to A, chromosome 17 at 35,848,546 bp
  • T to A, chromosome 17 at 64,037,585 bp
  • C to T, chromosome 18 at 34,611,284 bp
  • G to A, chromosome 18 at 37,768,587 bp
  • T to A, chromosome 18 at 67,506,646 bp
  • A to T, chromosome 19 at 34,593,797 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5561 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043118-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.