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Strain Name:
C57BL/6J-MtgxR5569Btlr/Mmmh
Stock Number:
043126-MU
Citation ID:
RRID:MMRRC_043126-MU
Other Names:
R5569 (G1), C57BL/6J-MtgxR5569Btlr
Major Collection:

Strain Information

Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Eri3
Name: exoribonuclease 3
Synonyms: PINT1, Prnpip1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140546
Homologene: 15403
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 21827
Homologene: 5159
Slc6a4
Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
Synonyms: 5-HTT, Htt, Sert
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15567
Homologene: 817
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 4,345,237 bp
  • A to T, chromosome 1 at 36,799,338 bp
  • A to G, chromosome 1 at 90,214,841 bp
  • C to T, chromosome 1 at 91,168,738 bp
  • A to T, chromosome 1 at 153,467,092 bp
  • T to A, chromosome 1 at 171,417,442 bp
  • T to A, chromosome 1 at 175,655,122 bp
  • C to T, chromosome 1 at 175,743,597 bp
  • A to T, chromosome 1 at 178,111,596 bp
  • A to G, chromosome 1 at 190,960,291 bp
  • G to A, chromosome 2 at 27,102,833 bp
  • T to A, chromosome 2 at 103,819,275 bp
  • A to G, chromosome 2 at 131,056,566 bp
  • T to A, chromosome 2 at 132,915,203 bp
  • C to T, chromosome 2 at 164,043,554 bp
  • T to C, chromosome 2 at 164,471,336 bp
  • T to C, chromosome 3 at 19,978,877 bp
  • C to A, chromosome 3 at 20,015,596 bp
  • T to A, chromosome 3 at 89,219,463 bp
  • G to T, chromosome 3 at 127,561,025 bp
  • A to G, chromosome 4 at 11,816,990 bp
  • C to T, chromosome 4 at 34,724,892 bp
  • T to C, chromosome 4 at 47,045,007 bp
  • T to A, chromosome 4 at 113,688,706 bp
  • T to C, chromosome 4 at 116,155,967 bp
  • T to C, chromosome 4 at 117,649,356 bp
  • T to C, chromosome 4 at 130,278,479 bp
  • A to G, chromosome 4 at 145,647,054 bp
  • C to T, chromosome 5 at 23,748,834 bp
  • A to G, chromosome 5 at 35,603,033 bp
  • A to G, chromosome 5 at 66,686,873 bp
  • T to A, chromosome 5 at 109,220,395 bp
  • A to G, chromosome 5 at 121,626,080 bp
  • A to T, chromosome 5 at 122,106,720 bp
  • C to T, chromosome 5 at 124,584,297 bp
  • A to T, chromosome 5 at 142,474,451 bp
  • A to G, chromosome 5 at 142,961,044 bp
  • T to C, chromosome 5 at 143,202,421 bp
  • T to A, chromosome 6 at 11,934,016 bp
  • T to C, chromosome 6 at 66,553,172 bp
  • C to T, chromosome 6 at 83,079,705 bp
  • T to A, chromosome 6 at 91,477,354 bp
  • A to G, chromosome 6 at 149,014,089 bp
  • A to T, chromosome 7 at 44,848,331 bp
  • T to C, chromosome 7 at 47,590,011 bp
  • A to G, chromosome 7 at 91,968,180 bp
  • A to T, chromosome 7 at 106,678,483 bp
  • T to A, chromosome 7 at 108,565,565 bp
  • G to A, chromosome 7 at 127,867,823 bp
  • G to A, chromosome 8 at 4,267,271 bp
  • C to T, chromosome 8 at 13,005,481 bp
  • T to A, chromosome 8 at 45,039,836 bp
  • T to C, chromosome 8 at 83,668,332 bp
  • A to T, chromosome 8 at 105,617,515 bp
  • A to T, chromosome 8 at 112,733,487 bp
  • A to G, chromosome 9 at 40,074,297 bp
  • A to T, chromosome 9 at 53,516,450 bp
  • A to G, chromosome 9 at 62,070,203 bp
  • T to C, chromosome 10 at 24,778,821 bp
  • G to A, chromosome 10 at 28,781,891 bp
  • G to A, chromosome 10 at 45,097,437 bp
  • A to G, chromosome 10 at 62,286,441 bp
  • A to G, chromosome 11 at 44,992,401 bp
  • A to T, chromosome 11 at 59,760,963 bp
  • A to T, chromosome 11 at 60,416,880 bp
  • A to T, chromosome 11 at 73,486,692 bp
  • A to T, chromosome 11 at 77,023,255 bp
  • G to A, chromosome 11 at 90,661,881 bp
  • C to T, chromosome 11 at 95,749,290 bp
  • T to A, chromosome 12 at 16,995,628 bp
  • A to G, chromosome 12 at 31,487,441 bp
  • A to T, chromosome 12 at 80,340,201 bp
  • A to C, chromosome 12 at 103,654,460 bp
  • A to G, chromosome 12 at 112,601,679 bp
  • A to G, chromosome 12 at 114,366,935 bp
  • C to A, chromosome 12 at 114,455,856 bp
  • C to A, chromosome 13 at 22,187,214 bp
  • C to T, chromosome 13 at 38,192,652 bp
  • A to G, chromosome 13 at 65,152,979 bp
  • T to C, chromosome 14 at 30,663,226 bp
  • A to T, chromosome 14 at 60,802,934 bp
  • A to T, chromosome 14 at 103,135,243 bp
  • T to C, chromosome 15 at 52,478,228 bp
  • A to C, chromosome 15 at 78,440,303 bp
  • T to A, chromosome 16 at 20,595,339 bp
  • T to C, chromosome 17 at 32,377,170 bp
  • C to T, chromosome 17 at 46,511,262 bp
  • T to C, chromosome 17 at 56,102,147 bp
  • G to T, chromosome 18 at 37,768,252 bp
  • A to C, chromosome 18 at 87,757,268 bp
  • T to A, chromosome 19 at 6,013,660 bp
  • T to C, chromosome 19 at 47,738,081 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5569 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043126-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.