Strain Name:
C57BL/6J-MtgxR5577Btlr/Mmmh
Stock Number:
043132-MU
Citation ID:
RRID:MMRRC_043132-MU
Other Names:
R5577 (G1), C57BL/6J-MtgxR5577Btlr
Major Collection:

Strain Information

Eno1
Name: enolase 1, alpha non-neuron
Synonyms: 2-phospho-D-glycerate hydrolase, alpha-enolase, Eno-1, MBP-1, c-Myc promoter binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13806
HGNC: HGNC:3350
Homologene: 134343
Myo1e
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Ppp2r5d
Name: protein phosphatase 2, regulatory subunit B', delta
Synonyms: TEG-271, Tex271, B'delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21770
VEGA: 17
HGNC: HGNC:9312
Homologene: 37661
Trappc8
Name: trafficking protein particle complex 8
Synonyms: 5033403J15Rik, D030074E01Rik, Trs85
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75964
VEGA: 18
Homologene: 40993
Sec24a
Name: Sec24 related gene family, member A (S. cerevisiae)
Synonyms: 9430090N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77371
Homologene: 70131
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Prdx6
Name: peroxiredoxin 6
Synonyms: acidic calcium-independent phospholipase A2, aiPLA2, GPx, Brp-12, Ltw4, Lvtw-4, Ltw-4, 1-Cys Prx, CP-3, Aop2, 1-cysPrx, 9430088D19Rik, NSGP, CC26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11758
Homologene: 3606
Gls
Name: glutaminase
Synonyms: B230365M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14660
HGNC: HGNC:4331
Homologene: 22726
Ccn3
Name: cellular communication network factor 3
Synonyms: C130088N23Rik, CCN3, Nov
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18133
HGNC: HGNC:7885
Homologene: 1884
Lrrk2
Name: leucine-rich repeat kinase 2
Synonyms: cI-46, LOC381026, 9330188B09Rik, D630001M17Rik, 4921513O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66725
Homologene: 18982
Akt2
Name: thymoma viral proto-oncogene 2
Synonyms: PKBbeta, PKB, 2410016A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11652
HGNC: HGNC:392
Homologene: 48773
Dmkn
Name: dermokine
Synonyms: cI-36, sk30, sk89, Dmkn, dermokine, 1110014F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73712
Homologene: 136295
Enpp7
Name: ectonucleotide pyrophosphatase/phosphodiesterase 7
Synonyms: Alk-SMase, LOC238011
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 238011
Homologene: 110852
Sqstm1
Name: sequestosome 1
Synonyms: A170, OSF-6, STAP, Osi, p62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18412
Homologene: 31202
Sec31a
Name: Sec31 homolog A (S. cerevisiae)
Synonyms: 1810024J13Rik, Sec31l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69162
Homologene: 42056
Clmn
Name: calmin
Synonyms: 9330188N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 94040
VEGA: 12
Homologene: 11683
Fancm
Name: Fanconi anemia, complementation group M
Synonyms: C730036B14Rik, D12Ertd364e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104806
VEGA: 12
Homologene: 35378
Zfp534
Name: zinc finger protein 534
Synonyms: Gm13159
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100503584
Homologene: 133076
Acr
Name: acrosin prepropeptide
Synonyms: preproacrosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11434
VEGA: 15
HGNC: HGNC:126
Homologene: 855
Tas1r3
Name: taste receptor, type 1, member 3
Synonyms: T1r3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 83771
Homologene: 12890
Cdh23
Name: cadherin 23 (otocadherin)
Synonyms: 4930542A03Rik, USH1D, mdfw, ahl, bob, nmf112, nmf181, nmf252, sals
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22295
Homologene: 11142
Nav3
Name: neuron navigator 3
Synonyms: Pomfil1p, POMFIL1, 4732483H20Rik, unc53H3, steerin 3, 9630020C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 260315
Homologene: 56688
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195046
Homologene: 133820
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100125586
Homologene: 129753
Klc4
Name: kinesin light chain 4
Synonyms: 1200014P03Rik, Knsl8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74764
VEGA: 17
Homologene: 12584
Fshr
Name: follicle stimulating hormone receptor
Synonyms: follicle-stimulating hormone receptor, Follitropin receptor, FSH-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14309
VEGA: 17
HGNC: HGNC:3969
Homologene: 117
Necab3
Name: N-terminal EF-hand calcium binding protein 3
Synonyms: XB51, 2900010M17Rik, Nip1, Apba2bp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56846
Homologene: 10992
Hdac7
Name: histone deacetylase 7
Synonyms: 5830434K02Rik, Hdac7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56233
Homologene: 9124
Tlr1
Name: toll-like receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21897
Homologene: 20694
9130023H24Rik
Name: RIKEN cDNA 9130023H24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043133
Cd177
Name: CD177 antigen
Synonyms: Pdp3, 1190003K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68891
Homologene: 49628
Or8b52
Name: olfactory receptor family 8 subfamily B member 52
Synonyms: GA_x6K02T2PVTD-32368166-32367237, MOR168-2P, Olfr917
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258183
VEGA: 9
Csrp3
Name: cysteine and glycine-rich protein 3
Synonyms: CRP3, muscle LIM protein, MLP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13009
HGNC: HGNC:2472
Homologene: 20742
Lgalsl
Name: galectin like
Synonyms: 1110067D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216551
Homologene: 8581
Lcn9
Name: lipocalin 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 77704
Homologene: 12681
Or5v1
Name: olfactory receptor family 5 subfamily V member 1
Synonyms: GA_x6K02T2PSCP-1956307-1957260, MOR249-2, Olfr110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258325
Homologene: 73968
Vmn2r43
Name: vomeronasal 2, receptor 43
Synonyms: EC2-V2R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381838
Homologene: 113703
Gm8730
Name: predicted pseudogene 8730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 667618
Gm3898
Name: predicted gene 3898
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100042548
VEGA: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 52,168,435 bp
  • A to G, chromosome 1 at 161,243,685 bp
  • T to A, chromosome 2 at 25,823,651 bp
  • A to T, chromosome 2 at 40,875,123 bp
  • T to C, chromosome 2 at 154,545,156 bp
  • T to A, chromosome 3 at 64,116,995 bp
  • T to G, chromosome 4 at 147,674,716 bp
  • C to A, chromosome 4 at 150,246,610 bp
  • T to C, chromosome 4 at 155,862,065 bp
  • T to A, chromosome 5 at 64,926,085 bp
  • T to C, chromosome 5 at 100,402,274 bp
  • T to C, chromosome 7 at 8,244,812 bp
  • A to T, chromosome 7 at 24,745,137 bp
  • G to A, chromosome 7 at 27,636,306 bp
  • T to C, chromosome 7 at 30,764,546 bp
  • T to C, chromosome 7 at 48,839,477 bp
  • A to C, chromosome 7 at 128,236,654 bp
  • G to T, chromosome 8 at 102,865,136 bp
  • A to G, chromosome 9 at 38,665,001 bp
  • T to A, chromosome 9 at 43,830,065 bp
  • T to C, chromosome 9 at 66,481,981 bp
  • G to A, chromosome 9 at 70,370,471 bp
  • A to G, chromosome 10 at 60,304,616 bp
  • A to T, chromosome 10 at 109,769,403 bp
  • G to A, chromosome 11 at 20,829,316 bp
  • T to C, chromosome 11 at 50,207,439 bp
  • A to T, chromosome 11 at 51,734,621 bp
  • T to A, chromosome 11 at 71,099,574 bp
  • T to A, chromosome 11 at 118,992,127 bp
  • T to A, chromosome 12 at 65,130,411 bp
  • G to T, chromosome 12 at 104,777,070 bp
  • T to A, chromosome 15 at 54,752,501 bp
  • C to A, chromosome 15 at 89,574,238 bp
  • A to T, chromosome 15 at 91,765,745 bp
  • A to T, chromosome 15 at 97,811,455 bp
  • T to A, chromosome 17 at 37,499,602 bp
  • G to T, chromosome 17 at 46,635,429 bp
  • T to C, chromosome 17 at 46,687,975 bp
  • A to T, chromosome 17 at 88,985,923 bp
  • G to C, chromosome 18 at 20,836,779 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5577 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043132-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.