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Strain Name:
C57BL/6J-MtgxR5581Btlr/Mmmh
Stock Number:
043135-MU
Citation ID:
RRID:MMRRC_043135-MU
Other Names:
R5581 (G1), C57BL/6J-MtgxR5581Btlr
Major Collection:

Strain Information

Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, MYH-beta/slow, beta-MHC, B-MHC, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Slit1
Name: slit guidance ligand 1
Synonyms: Slil1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20562
Homologene: 2302
Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Ptpn12
Name: protein tyrosine phosphatase, non-receptor type 12
Synonyms: P19-PTP, PTP-PEST, PTP-P19
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19248
HGNC: HGNC:9645
Homologene: 37691
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67039
Appbp2
Name: amyloid beta precursor protein binding protein 2
Synonyms: 1300003O07Rik, PAT1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66884
HGNC: HGNC:622
Homologene: 31378
Casr
Name: calcium-sensing receptor
Synonyms: CaR, Gprc2a, cation sensing receptor
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12374
HGNC: HGNC:1514
Homologene: 332
Pdia5
Name: protein disulfide isomerase associated 5
Synonyms: 2700053F16Rik, Pdir
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 72599
VEGA: 16
Homologene: 4957
Cdc123
Name: cell division cycle 123
Synonyms: G431001I09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98828
Homologene: 4394
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319565
Homologene: 56700
Plcz1
Name: phospholipase C, zeta 1
Synonyms: 1700041H07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 114875
Homologene: 23815
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Rnh1
Name: ribonuclease/angiogenin inhibitor 1
Synonyms: RNH
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 107702
Homologene: 2204
Filip1
Name: filamin A interacting protein 1
Synonyms: 5730485H21Rik, FILIP
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70598
Homologene: 6700
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329942
Homologene: 89034
Dixdc1
Name: DIX domain containing 1
Synonyms: 4930563F16Rik, Ccd1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330938
Homologene: 82369
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 207596
Homologene: 49796
Lvrn
Name: laeverin
Synonyms: 4833403I15Rik, Aqpep
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 74574
VEGA: 18
Homologene: 51352
Gm7052
Name: predicted gene 7052
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 631010
Or8g17
Name: olfactory receptor family 8 subfamily G member 17
Synonyms: M15, MOR171-10, GA_x6K02T2PVTD-32715386-32714466, Olfr146
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258742
VEGA: 9
Chfr
Name: checkpoint with forkhead and ring finger domains
Synonyms: RNF116, 5730484M20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231600
Homologene: 10078
Bcl11a
Name: BCL11 transcription factor A
Synonyms: mouse myeloid leukemia gene, COUP-TF interacting protein 1, CTIP1, Evi9, 2810047E18Rik, D930021L15Rik, Evi9c, Evi9b, Evi9a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14025
Homologene: 11284
Ndufb11b
Name: NADH:ubiquinone oxidoreductase subunit B11B
Synonyms: 1700029P11Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 66346
Homologene: 32635
Gm10705
Name: predicted gene 10705
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100042355
Homologene: 138801
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 2 at 5,833,116 bp
  • T to A, chromosome 2 at 82,998,128 bp
  • T to C, chromosome 3 at 87,207,948 bp
  • A to G, chromosome 4 at 128,493,447 bp
  • T to A, chromosome 5 at 21,015,726 bp
  • T to C, chromosome 5 at 110,153,282 bp
  • C to T, chromosome 5 at 124,584,297 bp
  • A to G, chromosome 6 at 140,023,125 bp
  • T to C, chromosome 7 at 141,163,381 bp
  • C to T, chromosome 9 at 35,504,081 bp
  • T to A, chromosome 9 at 39,018,702 bp
  • A to G, chromosome 9 at 50,669,480 bp
  • C to A, chromosome 9 at 59,972,458 bp
  • C to T, chromosome 9 at 79,819,760 bp
  • A to G, chromosome 11 at 24,163,932 bp
  • G to C, chromosome 11 at 85,210,095 bp
  • T to G, chromosome 12 at 75,945,085 bp
  • T to A, chromosome 12 at 83,651,572 bp
  • T to A, chromosome 14 at 54,978,954 bp
  • T to C, chromosome 15 at 81,980,836 bp
  • T to C, chromosome 16 at 35,449,442 bp
  • A to G, chromosome 16 at 36,494,744 bp
  • A to T, chromosome 17 at 22,039,743 bp
  • A to G, chromosome 18 at 46,890,865 bp
  • A to G, chromosome 19 at 41,616,663 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5581 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043135-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.