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Strain Name:
C57BL/6J-MtgxR5586Btlr/Mmmh
Stock Number:
043140-MU
Citation ID:
RRID:MMRRC_043140-MU
Other Names:
R5586 (G1), C57BL/6J-MtgxR5586Btlr
Major Collection:

Strain Information

Kif3c
Name: kinesin family member 3C
Synonyms: N-4 kinesin
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16570
VEGA: 12
HGNC: HGNC:6321
Homologene: 55640
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Calb1
Name: calbindin 1
Synonyms: Brain-2, Calb-1, Calb, CB, calbindin-D (28k), calbindin, CalbindinD28K
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12307
HGNC: HGNC:1434
Homologene: 21026
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Zfp423
Name: zinc finger protein 423
Synonyms: Roaz, ataxia1, Zfp104, Ebfaz
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94187
Homologene: 9010
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 40,225,251 bp
  • T to C, chromosome 1 at 63,745,906 bp
  • T to A, chromosome 1 at 110,929,857 bp
  • T to A, chromosome 1 at 133,379,305 bp
  • C to T, chromosome 1 at 151,717,556 bp
  • T to C, chromosome 1 at 155,312,863 bp
  • T to A, chromosome 1 at 172,105,222 bp
  • T to A, chromosome 2 at 21,212,390 bp
  • T to C, chromosome 2 at 26,915,951 bp
  • T to A, chromosome 2 at 65,707,295 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • C to T, chromosome 2 at 180,659,652 bp
  • G to A, chromosome 3 at 50,443,083 bp
  • T to C, chromosome 3 at 55,631,971 bp
  • C to T, chromosome 3 at 115,990,051 bp
  • G to A, chromosome 3 at 154,270,165 bp
  • A to T, chromosome 4 at 15,900,811 bp
  • T to A, chromosome 4 at 101,910,120 bp
  • A to G, chromosome 4 at 116,435,563 bp
  • C to T, chromosome 4 at 141,905,131 bp
  • A to T, chromosome 5 at 17,711,424 bp
  • T to A, chromosome 5 at 81,724,147 bp
  • T to C, chromosome 5 at 86,135,047 bp
  • A to G, chromosome 5 at 144,282,139 bp
  • T to A, chromosome 5 at 146,484,457 bp
  • G to T, chromosome 6 at 13,836,001 bp
  • A to C, chromosome 6 at 123,825,296 bp
  • A to G, chromosome 6 at 124,398,901 bp
  • A to T, chromosome 7 at 25,917,012 bp
  • A to G, chromosome 7 at 29,577,728 bp
  • G to A, chromosome 7 at 35,551,527 bp
  • G to T, chromosome 7 at 42,045,681 bp
  • T to A, chromosome 7 at 49,826,751 bp
  • A to G, chromosome 7 at 105,024,221 bp
  • T to C, chromosome 7 at 109,905,721 bp
  • G to T, chromosome 8 at 3,510,389 bp
  • A to T, chromosome 8 at 9,988,318 bp
  • T to A, chromosome 8 at 87,859,340 bp
  • T to A, chromosome 8 at 93,127,599 bp
  • C to T, chromosome 9 at 8,901,460 bp
  • A to T, chromosome 9 at 21,034,820 bp
  • A to G, chromosome 9 at 39,591,263 bp
  • T to C, chromosome 9 at 59,949,201 bp
  • C to T, chromosome 9 at 92,266,392 bp
  • C to T, chromosome 9 at 100,985,803 bp
  • A to T, chromosome 9 at 107,898,695 bp
  • T to C, chromosome 9 at 108,356,462 bp
  • C to A, chromosome 10 at 62,878,294 bp
  • A to G, chromosome 10 at 80,359,464 bp
  • A to T, chromosome 10 at 80,719,130 bp
  • A to G, chromosome 10 at 86,656,545 bp
  • T to A, chromosome 10 at 90,077,064 bp
  • T to A, chromosome 10 at 92,972,611 bp
  • T to C, chromosome 10 at 116,353,827 bp
  • A to G, chromosome 11 at 29,503,484 bp
  • A to G, chromosome 11 at 35,827,386 bp
  • T to C, chromosome 11 at 50,203,022 bp
  • G to A, chromosome 11 at 59,001,468 bp
  • G to T, chromosome 11 at 69,226,256 bp
  • G to A, chromosome 11 at 94,364,421 bp
  • T to G, chromosome 11 at 101,106,339 bp
  • T to C, chromosome 11 at 101,733,036 bp
  • T to A, chromosome 11 at 107,486,475 bp
  • T to A, chromosome 11 at 116,250,862 bp
  • A to T, chromosome 12 at 3,389,656 bp
  • G to A, chromosome 12 at 17,416,828 bp
  • T to A, chromosome 12 at 30,003,142 bp
  • A to G, chromosome 12 at 52,519,912 bp
  • A to G, chromosome 12 at 69,307,693 bp
  • T to C, chromosome 13 at 19,594,548 bp
  • C to A, chromosome 13 at 21,555,096 bp
  • T to C, chromosome 13 at 21,949,680 bp
  • A to C, chromosome 13 at 40,860,953 bp
  • A to G, chromosome 14 at 20,520,690 bp
  • C to A, chromosome 14 at 27,506,711 bp
  • A to G, chromosome 14 at 30,870,819 bp
  • A to T, chromosome 14 at 61,206,441 bp
  • A to T, chromosome 15 at 7,884,288 bp
  • A to T, chromosome 15 at 39,099,913 bp
  • T to C, chromosome 15 at 77,526,399 bp
  • A to G, chromosome 15 at 100,787,164 bp
  • A to T, chromosome 15 at 102,131,937 bp
  • A to T, chromosome 15 at 102,346,676 bp
  • A to G, chromosome 16 at 20,517,289 bp
  • A to T, chromosome 16 at 23,889,737 bp
  • A to G, chromosome 16 at 23,973,176 bp
  • A to T, chromosome 16 at 32,041,519 bp
  • A to T, chromosome 16 at 35,157,116 bp
  • A to G, chromosome 16 at 57,122,130 bp
  • A to T, chromosome 16 at 91,009,977 bp
  • T to C, chromosome 17 at 23,808,138 bp
  • A to T, chromosome 17 at 37,610,254 bp
  • A to G, chromosome 17 at 48,264,899 bp
  • A to G, chromosome 17 at 65,824,568 bp
  • A to G, chromosome 17 at 66,920,196 bp
  • T to A, chromosome 17 at 70,818,161 bp
  • C to T, chromosome 18 at 37,308,981 bp
  • A to G, chromosome 18 at 37,401,114 bp
  • C to T, chromosome 18 at 63,664,325 bp
  • T to A, chromosome 19 at 11,013,674 bp
  • A to T, chromosome 19 at 13,454,382 bp
  • C to T, chromosome 19 at 34,594,277 bp
  • C to T, chromosome 19 at 38,814,695 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5586 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043140-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.