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Strain Name:
C57BL/6J-MtgxR5590Btlr/Mmmh
Stock Number:
043143-MU
Citation ID:
RRID:MMRRC_043143-MU
Other Names:
R5590 (G1), C57BL/6J-MtgxR5590Btlr
Major Collection:

Strain Information

Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Uso1
Name: USO1 vesicle docking factor
Synonyms: transcytosis associated protein p115, TAP, Vdp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56041
Homologene: 2754
Tnrc6b
Name: trinucleotide repeat containing 6b
Synonyms: A730065C02Rik, D230019K20Rik, 2700090M07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 213988
VEGA: 15
Homologene: 66194
Zfa-ps
Name: zinc finger protein, autosomal, pseudogene
Synonyms: Zfa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22639
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Adgre1
Name: adhesion G protein-coupled receptor E1
Synonyms: F4/80, DD7A5-7, TM7LN3, EGF-TM7, Ly71, Emr1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13733
VEGA: 17
HGNC: HGNC:3336
Homologene: 1493
Pzp
Name: PZP, alpha-2-macroglobulin like
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11287
Homologene: 104112
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 52,794,661 bp
  • A to T, chromosome 1 at 55,084,769 bp
  • G to A, chromosome 1 at 91,427,856 bp
  • T to G, chromosome 1 at 94,008,301 bp
  • A to T, chromosome 1 at 106,392,927 bp
  • A to T, chromosome 1 at 130,882,516 bp
  • G to T, chromosome 1 at 153,094,061 bp
  • T to C, chromosome 1 at 162,592,679 bp
  • T to C, chromosome 1 at 172,133,212 bp
  • A to T, chromosome 1 at 174,175,770 bp
  • T to C, chromosome 2 at 67,514,035 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to T, chromosome 2 at 105,127,284 bp
  • A to T, chromosome 3 at 9,699,422 bp
  • A to T, chromosome 3 at 32,563,847 bp
  • T to C, chromosome 3 at 64,504,076 bp
  • A to C, chromosome 3 at 88,056,533 bp
  • A to G, chromosome 3 at 89,088,027 bp
  • A to G, chromosome 3 at 90,454,842 bp
  • C to T, chromosome 3 at 133,476,480 bp
  • T to C, chromosome 4 at 45,106,679 bp
  • T to C, chromosome 4 at 72,124,971 bp
  • A to T, chromosome 4 at 109,432,987 bp
  • T to A, chromosome 4 at 120,715,885 bp
  • T to G, chromosome 4 at 124,610,487 bp
  • T to C, chromosome 4 at 126,048,695 bp
  • G to A, chromosome 4 at 133,421,563 bp
  • T to A, chromosome 4 at 139,642,104 bp
  • A to T, chromosome 5 at 36,525,045 bp
  • C to A, chromosome 5 at 65,422,874 bp
  • A to G, chromosome 5 at 92,180,608 bp
  • A to T, chromosome 5 at 92,239,139 bp
  • G to T, chromosome 5 at 110,381,618 bp
  • A to G, chromosome 5 at 134,223,501 bp
  • A to G, chromosome 5 at 144,782,265 bp
  • C to A, chromosome 5 at 145,865,977 bp
  • A to G, chromosome 5 at 146,845,242 bp
  • A to G, chromosome 6 at 16,834,200 bp
  • G to T, chromosome 6 at 17,548,782 bp
  • A to G, chromosome 6 at 48,474,491 bp
  • A to T, chromosome 6 at 70,672,283 bp
  • A to T, chromosome 6 at 118,687,182 bp
  • C to T, chromosome 6 at 121,676,932 bp
  • A to C, chromosome 6 at 128,523,796 bp
  • G to A, chromosome 7 at 16,833,874 bp
  • T to C, chromosome 7 at 46,653,792 bp
  • A to G, chromosome 7 at 50,279,611 bp
  • G to T, chromosome 7 at 96,797,400 bp
  • C to A, chromosome 7 at 96,797,401 bp
  • C to T, chromosome 7 at 120,544,772 bp
  • A to T, chromosome 7 at 126,107,078 bp
  • T to A, chromosome 7 at 126,979,532 bp
  • T to A, chromosome 7 at 140,943,412 bp
  • T to C, chromosome 8 at 22,351,452 bp
  • G to A, chromosome 8 at 61,361,732 bp
  • A to C, chromosome 8 at 70,539,707 bp
  • G to A, chromosome 8 at 70,813,767 bp
  • G to A, chromosome 8 at 94,345,788 bp
  • T to G, chromosome 8 at 120,581,747 bp
  • T to C, chromosome 8 at 123,303,963 bp
  • A to T, chromosome 9 at 30,951,336 bp
  • T to C, chromosome 9 at 32,728,798 bp
  • T to A, chromosome 9 at 38,672,965 bp
  • T to A, chromosome 9 at 45,265,626 bp
  • T to A, chromosome 9 at 75,457,141 bp
  • T to C, chromosome 9 at 99,358,413 bp
  • G to A, chromosome 9 at 124,028,793 bp
  • T to C, chromosome 10 at 40,581,341 bp
  • T to C, chromosome 10 at 52,544,315 bp
  • A to T, chromosome 10 at 53,108,178 bp
  • T to A, chromosome 10 at 61,343,710 bp
  • A to G, chromosome 10 at 63,120,048 bp
  • A to T, chromosome 10 at 63,927,066 bp
  • A to T, chromosome 10 at 77,870,365 bp
  • T to C, chromosome 10 at 84,375,255 bp
  • T to C, chromosome 10 at 111,272,168 bp
  • T to C, chromosome 10 at 127,664,657 bp
  • G to A, chromosome 11 at 22,517,450 bp
  • A to T, chromosome 11 at 51,627,962 bp
  • T to A, chromosome 11 at 78,155,032 bp
  • A to G, chromosome 11 at 87,856,655 bp
  • A to G, chromosome 11 at 101,124,032 bp
  • A to G, chromosome 11 at 105,923,306 bp
  • T to C, chromosome 12 at 55,304,472 bp
  • T to C, chromosome 12 at 74,976,689 bp
  • C to A, chromosome 12 at 112,051,980 bp
  • T to A, chromosome 12 at 116,059,727 bp
  • A to T, chromosome 13 at 40,705,813 bp
  • T to A, chromosome 13 at 61,201,812 bp
  • T to A, chromosome 13 at 104,148,241 bp
  • A to T, chromosome 14 at 56,373,380 bp
  • A to C, chromosome 14 at 103,123,355 bp
  • A to T, chromosome 15 at 10,462,277 bp
  • G to A, chromosome 15 at 78,437,302 bp
  • C to T, chromosome 15 at 80,876,502 bp
  • T to A, chromosome 16 at 16,891,577 bp
  • T to A, chromosome 16 at 31,989,513 bp
  • A to G, chromosome 16 at 32,103,898 bp
  • T to A, chromosome 16 at 34,879,352 bp
  • A to G, chromosome 16 at 35,801,663 bp
  • A to G, chromosome 16 at 59,085,997 bp
  • A to G, chromosome 17 at 29,839,867 bp
  • A to G, chromosome 17 at 33,961,221 bp
  • T to C, chromosome 17 at 34,740,335 bp
  • A to T, chromosome 17 at 35,526,680 bp
  • A to G, chromosome 17 at 46,258,871 bp
  • T to A, chromosome 17 at 57,445,034 bp
  • G to A, chromosome 18 at 37,961,327 bp
  • A to T, chromosome 19 at 11,569,488 bp
  • A to G, chromosome 19 at 12,059,278 bp
  • A to T, chromosome 19 at 27,802,380 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5590 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043143-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.