Strain Name:
C57BL/6J-MtgxR5596Btlr/Mmmh
Stock Number:
043148-MU
Citation ID:
RRID:MMRRC_043148-MU
Other Names:
R5596 (G1), C57BL/6J-MtgxR5596Btlr
Major Collection:

Gene Information

Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Gjd2
Name: gap junction protein, delta 2
Synonyms: connexin36, Cx36, Gja9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14617
Homologene: 7734
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Capzb
Name: capping actin protein of muscle Z-line subunit beta
Synonyms: CPbeta2, CPB2, CPbeta1, CPB1, 1700120C01Rik, Cappb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12345
HGNC: HGNC:1491
Homologene: 3620
Cacng7
Name: calcium channel, voltage-dependent, gamma subunit 7
Synonyms: TARP gamma 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 81904
Homologene: 12895
Enox1
Name: ecto-NOX disulfide-thiol exchanger 1
Synonyms: D230005D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 239188
VEGA: 14
Homologene: 56793
Ptpn1
Name: protein tyrosine phosphatase, non-receptor type 1
Synonyms: PTP-1B, PTP1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19246
HGNC: HGNC:9642
Homologene: 2119
Herc1
Name: HECT and RLD domain containing E3 ubiquitin protein ligase family member 1
Synonyms: D130015N03Rik, 2810449H11Rik, tbl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235439
HGNC: HGNC:4867
Homologene: 31207
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Tnfrsf9
Name: tumor necrosis factor receptor superfamily, member 9
Synonyms: 4-1BB, CDw137, Ly63, Cd137, ILA, A930040I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21942
Homologene: 1199
Irx4
Name: Iroquois homeobox 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 50916
HGNC: HGNC:6129
Homologene: 9485
Bub1b
Name: BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms: BUBR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12236
HGNC: HGNC:1149
Homologene: 933
Fbxw4
Name: F-box and WD-40 domain protein 4
Synonyms: dactylin, Fbw4, dactylyn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 30838
Homologene: 32197
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329650
Homologene: 43143
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1537Clo, b2b1565Clo, b2b1154Clo, b2b1134Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 229003
Homologene: 14118
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: EG640524, Spnb5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 640524
Homologene: 41150
Impg1
Name: interphotoreceptor matrix proteoglycan 1
Synonyms: IMP150, SPACR, A930015H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 63859
HGNC: HGNC:6055
Homologene: 1201
Lmntd1
Name: lamin tail domain containing 1
Synonyms: Lmna-rs1, 4933403M22Rik, Ifltd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74071
Homologene: 69453
Abca15
Name: ATP-binding cassette, sub-family A (ABC1), member 15
Synonyms: 4930500I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320631
Homologene: 87255
Sycp2
Name: synaptonemal complex protein 2
Synonyms: 3830402K23Rik, 4930518F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320558
Homologene: 8604
Cfap69
Name: cilia and flagella associated protein 69
Synonyms: A330021E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 207686
Homologene: 11718
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 81877
Homologene: 49589
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp-beta, Nalp4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384752
Vwa5a
Name: von Willebrand factor A domain containing 5A
Synonyms: BCSC-1, 5830475I06Rik, Loh11cr2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67776
HGNC: HGNC:6658
Homologene: 18222
Tmcc2
Name: transmembrane and coiled-coil domains 2
Synonyms: 1110063G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 68875
Homologene: 8905
Kcnip1
Name: Kv channel-interacting protein 1
Synonyms: 2900046L02Rik, KCHIP1, 3202002F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70357
Homologene: 22824
Ccdc149
Name: coiled-coil domain containing 149
Synonyms: LOC242997, Gm447
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100503884
Homologene: 65264
Pbld2
Name: phenazine biosynthesis-like protein domain containing 2
Synonyms: 3110049J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67307
Xkr5
Name: X-linked Kx blood group related 5
Synonyms: 5430438H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319581
Homologene: 52219
Fdxr
Name: ferredoxin reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14149
HGNC: HGNC:3642
Homologene: 3033
Musk
Name: muscle, skeletal, receptor tyrosine kinase
Synonyms: MDK4, Nsk1, Nsk2, Nsk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18198
HGNC: HGNC:7525
Homologene: 4084
Tdpoz4
Name: TD and POZ domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 399675
Homologene: 130121
Sstr4
Name: somatostatin receptor 4
Synonyms: sst4, Smstr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20608
Homologene: 20286
Fam186b
Name: family with sequence similarity 186, member B
Synonyms: EG545136
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 545136
VEGA: 15
Homologene: 69502
Creb3l3
Name: cAMP responsive element binding protein 3-like 3
Synonyms: CREB-H, D10Bur1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 208677
Homologene: 13080
Cyp2j8
Name: cytochrome P450, family 2, subfamily j, polypeptide 8
Synonyms: OTTMUSG00000007938, Cyp2j8-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 665095
HGNC: HGNC:2634
Homologene: 133819
Abraxas1
Name: BRCA1 A complex subunit
Synonyms: 5630400M01Rik, 3830405G04Rik, Ccdc98, Fam175a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 70681
Homologene: 16376
Erich3
Name: glutamate rich 3
Synonyms: 5031409G23Rik, 4922501L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 209601
Homologene: 27877
C1qtnf7
Name: C1q and tumor necrosis factor related protein 7
Synonyms: 5530401N20Rik, 8430425G24Rik, CTRP7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 109323
Homologene: 12900
Sap18b
Name: Sin3-associated polypeptide 18B
Synonyms: Gm10094
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100041953
Homologene: 4289
Mir7027
Name: microRNA 7027
Synonyms: mmu-mir-7027
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 102465621
VEGA: 5
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 132,360,483 bp
  • C to A, chromosome 2 at 114,011,484 bp
  • T to C, chromosome 2 at 118,630,982 bp
  • G to A, chromosome 2 at 120,046,484 bp
  • G to T, chromosome 2 at 148,395,732 bp
  • T to C, chromosome 2 at 167,974,763 bp
  • T to C, chromosome 2 at 178,354,953 bp
  • A to T, chromosome 2 at 181,237,289 bp
  • T to A, chromosome 3 at 59,252,350 bp
  • A to T, chromosome 3 at 93,797,499 bp
  • A to G, chromosome 3 at 153,938,607 bp
  • A to T, chromosome 3 at 154,727,396 bp
  • T to C, chromosome 4 at 58,373,036 bp
  • C to T, chromosome 4 at 88,630,055 bp
  • T to A, chromosome 4 at 96,507,341 bp
  • T to G, chromosome 4 at 139,279,427 bp
  • T to A, chromosome 4 at 143,654,455 bp
  • T to C, chromosome 4 at 150,929,874 bp
  • A to G, chromosome 5 at 5,626,020 bp
  • A to T, chromosome 5 at 43,515,970 bp
  • A to G, chromosome 5 at 52,404,151 bp
  • A to T, chromosome 5 at 100,818,537 bp
  • T to A, chromosome 5 at 114,406,160 bp
  • A to G, chromosome 6 at 18,268,096 bp
  • T to A, chromosome 6 at 145,413,414 bp
  • A to T, chromosome 7 at 3,366,904 bp
  • A to T, chromosome 7 at 10,382,024 bp
  • A to G, chromosome 7 at 120,401,749 bp
  • A to G, chromosome 8 at 18,950,652 bp
  • G to T, chromosome 8 at 95,825,370 bp
  • A to T, chromosome 9 at 38,722,578 bp
  • T to TTN, chromosome 9 at 66,434,063 bp
  • G to A, chromosome 9 at 79,703,759 bp
  • A to T, chromosome 9 at 80,345,218 bp
  • C to T, chromosome 10 at 63,072,012 bp
  • T to G, chromosome 10 at 81,085,047 bp
  • T to C, chromosome 11 at 33,630,597 bp
  • A to T, chromosome 11 at 115,270,326 bp
  • A to G, chromosome 13 at 49,592,338 bp
  • A to G, chromosome 13 at 73,267,680 bp
  • A to G, chromosome 14 at 77,579,053 bp
  • G to T, chromosome 15 at 28,343,608 bp
  • A to T, chromosome 15 at 99,271,289 bp
  • T to A, chromosome 17 at 34,688,804 bp
  • T to C, chromosome 19 at 45,579,259 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5596 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043148-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.