Strain Name:
C57BL/6J-MtgxR5649Btlr/Mmmh
Stock Number:
043170-MU
Citation ID:
RRID:MMRRC_043170-MU
Other Names:
R5649 (G1), C57BL/6J-MtgxR5649Btlr
Major Collection:

Gene Information

Myo5a
Name: myosin VA
Synonyms: 9630007J19Rik, MyoVA, flail, MVa, Myo5, Dbv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Abca12
Name: ATP-binding cassette, sub-family A (ABC1), member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74591
Homologene: 45441
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66860
Homologene: 18946
Atl3
Name: atlastin GTPase 3
Synonyms: 5730596K20Rik, 4633402C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 109168
VEGA: 19
Homologene: 9149
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228829
Homologene: 9507
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, D330028K02Rik, Calmbp1, Aspm, MCPH5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12316
Homologene: 7650
Dock4
Name: dedicator of cytokinesis 4
Synonyms: EST N28122, 6330411N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238130
VEGA: 12
Homologene: 56680
D6Wsu163e
Name: DNA segment, Chr 6, Wayne State University 163, expressed
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 28040
HGNC: HGNC:1184
Homologene: 10685
Gpihbp1
Name: GPI-anchored HDL-binding protein 1
Synonyms: 1110002J19Rik, GPI-HBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 68453
Homologene: 136164
Olfm3
Name: olfactomedin 3
Synonyms: B230206G02Rik, optimedin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229759
Homologene: 17103
Mrpl55
Name: mitochondrial ribosomal protein L55
Synonyms: 2810038N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 67212
Homologene: 12098
Cnot3
Name: CCR4-NOT transcription complex, subunit 3
Synonyms: A930039N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232791
HGNC: HGNC:7879
Homologene: 133900
0610009B22Rik
Name: RIKEN cDNA 0610009B22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 66050
Homologene: 133292
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Slc45a2
Name: solute carrier family 45, member 2
Synonyms: Oca4, bls, Matp, Aim1, Dbr, blanc-sale, Aim-1, dominant brown
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 22293
Homologene: 9412
Dennd4a
Name: DENN/MADD domain containing 4A
Synonyms: F730015K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 102442
VEGA: 9
Homologene: 55933
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: A530024P18Rik, myomaxin, Cmya3, 2310008C07Rik, mXin beta, 2310003D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241431
Homologene: 19388
Csf3r
Name: colony stimulating factor 3 receptor (granulocyte)
Synonyms: Cd114, Csfgr, G-CSFR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12986
HGNC: HGNC:2439
Homologene: 601
Phf11c
Name: PHD finger protein 11C
Synonyms: Gm6907
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 628705
Homologene: 87807
Tmem30b
Name: transmembrane protein 30B
Synonyms: 9130011B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238257
VEGA: 12
Homologene: 133755
Olfr304
Name: olfactory receptor 304
Synonyms: GA_x6K02T2NHDJ-9721756-9722757, MOR219-3P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258089
Homologene: 121542
Slc15a2
Name: solute carrier family 15 (H+/peptide transporter), member 2
Synonyms: 8430408C16Rik, Pept2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 57738
Homologene: 56912
Vmn1r53
Name: vomeronasal 1 receptor 53
Synonyms: V1rb3, VN5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 113853
Homologene: 113975
Thbs2
Name: thrombospondin 2
Synonyms: Thrombospondin-2, Thbs-2, TSP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 21826
VEGA: 17
Homologene: 2438
Ssc5d
Name: scavenger receptor cysteine rich family, 5 domains
Synonyms: A430110N23Rik, s5d-srcrb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269855
Homologene: 77555
Them4
Name: thioesterase superfamily member 4
Synonyms: 4921507I02Rik, 2700077M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 75778
Homologene: 14208
Col5a1
Name: collagen, type V, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12831
HGNC: HGNC:2209
Homologene: 55434
Rgsl1
Name: regulator of G-protein signaling like 1
Synonyms: 4930415K13Rik, Rgsl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240816
Homologene: 129962
Hpn
Name: hepsin
Synonyms: Hlb320
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 15451
HGNC: HGNC:5155
Homologene: 20498
Apc2
Name: adenomatosis polyposis coli 2
Synonyms: APCL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 23805
Homologene: 4299
Itih3
Name: inter-alpha trypsin inhibitor, heavy chain 3
Synonyms: Itih-3, Intin3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 16426
HGNC: HGNC:6168
Homologene: 1669
Xkr5
Name: X-linked Kx blood group related 5
Synonyms: 5430438H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 319581
Homologene: 52219
Kif28
Name: kinesin family member 28
Synonyms: LOC383592, Gm1305
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 383592
Homologene: 130945
Plbd1
Name: phospholipase B domain containing 1
Synonyms: 1100001H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 66857
Homologene: 11745
Cyp24a1
Name: cytochrome P450, family 24, subfamily a, polypeptide 1
Synonyms: Cyp24, 25-hydroxyvitamin D-24-hydroxylase, CP24, 24-OHase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 13081
HGNC: HGNC:2602
Homologene: 68094
Wdr86
Name: WD repeat domain 86
Synonyms: 2810046M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269633
Homologene: 90112
Vmn1r29
Name: vomeronasal 1 receptor 29
Synonyms: V1rc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 113859
Homologene: 123821
Olfr695
Name: olfactory receptor 695
Synonyms: GA_x6K02T2PBJ9-9092181-9091234, MOR283-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258591
Homologene: 133597
Ttc29
Name: tetratricopeptide repeat domain 29
Synonyms: 1700031F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 73301
Homologene: 12918
Poglut1
Name: protein O-glucosyltransferase 1
Synonyms: Rumi, Ktelc1, wsnp, 9630046K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 224143
Homologene: 41353
Fancg
Name: Fanconi anemia, complementation group G
Synonyms: Xrcc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 60534
HGNC: HGNC:3588
Homologene: 3402
Cdh22
Name: cadherin 22
Synonyms: PB-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 104010
Homologene: 23148
Dlg2
Name: discs large MAGUK scaffold protein 2
Synonyms: LOC382816, A330103J02Rik, B230218P12Rik, Gm21505, Dlgh2, B330007M19Rik, PSD93, Chapsyn-110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 23859
HGNC: HGNC:2901
Homologene: 1046
Zfp607b
Name: zinc finger protein 607B
Synonyms: C030039L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 112415
Homologene: 134321
Pcdha12
Name: protocadherin alpha 12
Synonyms: Pcdha13, Cnr5, Crnr5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 192164
HGNC: HGNC:8667
Homologene: 135870
Ighd2-8
Name: immunoglobulin heavy diversity 2-8
Synonyms: Gm16782
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 777664
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 71,291,342 bp
  • G to A, chromosome 1 at 139,479,669 bp
  • G to A, chromosome 1 at 153,825,893 bp
  • A to G, chromosome 1 at 179,697,771 bp
  • A to G, chromosome 2 at 27,951,456 bp
  • G to A, chromosome 2 at 59,820,326 bp
  • A to G, chromosome 2 at 67,516,895 bp
  • T to A, chromosome 2 at 156,251,768 bp
  • G to T, chromosome 2 at 165,116,280 bp
  • T to C, chromosome 2 at 170,496,309 bp
  • A to T, chromosome 3 at 94,331,544 bp
  • A to G, chromosome 3 at 115,096,924 bp
  • A to G, chromosome 4 at 43,008,736 bp
  • A to G, chromosome 4 at 126,043,814 bp
  • A to G, chromosome 5 at 21,901,625 bp
  • A to T, chromosome 5 at 24,718,087 bp
  • C to G, chromosome 6 at 58,307,691 bp
  • G to A, chromosome 6 at 90,223,760 bp
  • T to A, chromosome 6 at 126,955,111 bp
  • T to A, chromosome 6 at 136,616,989 bp
  • T to C, chromosome 7 at 3,658,083 bp
  • T to A, chromosome 7 at 4,926,518 bp
  • T to G, chromosome 7 at 27,703,981 bp
  • T to C, chromosome 7 at 31,099,332 bp
  • T to C, chromosome 7 at 86,386,313 bp
  • T to A, chromosome 7 at 91,968,239 bp
  • T to C, chromosome 7 at 106,714,166 bp
  • T to C, chromosome 8 at 18,933,966 bp
  • G to A, chromosome 8 at 78,246,313 bp
  • C to A, chromosome 9 at 64,851,209 bp
  • A to G, chromosome 9 at 75,171,719 bp
  • T to A, chromosome 10 at 80,314,138 bp
  • T to C, chromosome 11 at 51,685,972 bp
  • T to A, chromosome 11 at 59,204,571 bp
  • T to C, chromosome 12 at 40,844,540 bp
  • G to T, chromosome 12 at 73,546,166 bp
  • A to G, chromosome 12 at 113,450,867 bp
  • T to C, chromosome 14 at 30,921,418 bp
  • T to C, chromosome 14 at 59,385,532 bp
  • C to T, chromosome 15 at 11,012,607 bp
  • C to A, chromosome 15 at 75,597,720 bp
  • A to T, chromosome 16 at 36,772,110 bp
  • A to G, chromosome 16 at 38,531,811 bp
  • T to C, chromosome 17 at 14,689,953 bp
  • A to T, chromosome 18 at 37,022,415 bp
  • C to A, chromosome 19 at 7,532,227 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5649 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043170-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.