Strain Name:
C57BL/6J-MtgxR5709Btlr/Mmmh
Stock Number:
043184-MU
Citation ID:
RRID:MMRRC_043184-MU
Other Names:
R5709 (G1), C57BL/6J-MtgxR5709Btlr
Major Collection:

Strain Information

Zmpste24
Name: zinc metallopeptidase, STE24
Synonyms: A530043O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230709
Homologene: 4277
Matr3
Name: matrin 3
Synonyms: D030046F20Rik, 2810017I02Rik, 1110061A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17184
HGNC: HGNC:6912
Homologene: 7830
Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Rpl23
Name: ribosomal protein L23
Synonyms: 2810009A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 65019
Homologene: 68103
Mkks
Name: McKusick-Kaufman syndrome
Synonyms: 1300013E18Rik, Bbs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 59030
HGNC: HGNC:7108
Homologene: 10318
Usp1
Name: ubiquitin specific peptidase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230484
Homologene: 2528
Rps6kc1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: RPK118, B130003F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320119
Homologene: 8244
Spred2
Name: sprouty-related EVH1 domain containing 2
Synonyms: Spred-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 114716
Homologene: 24918
Sbno2
Name: strawberry notch 2
Synonyms: Stno
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216161
VEGA: 10
Homologene: 8981
Shb
Name: src homology 2 domain-containing transforming protein B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230126
Homologene: 74458
Gpc1
Name: glypican 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14733
HGNC: HGNC:4449
Homologene: 20477
Rcn1
Name: reticulocalbin 1
Synonyms: Rcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19672
HGNC: HGNC:9934
Homologene: 20637
Armt1
Name: acidic residue methyltransferase 1
Synonyms: 1700052N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73419
Homologene: 41557
Cpne6
Name: copine VI
Synonyms: neuronal copine
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12891
HGNC: HGNC:2319
Homologene: 81815
Car14
Name: carbonic anhydrase 14
Synonyms: CA XIV
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23831
HGNC: HGNC:1372
Homologene: 69105
Zfp386
Name: zinc finger protein 386 (Kruppel-like)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56220
Homologene: 51668
Dpp9
Name: dipeptidylpeptidase 9
Synonyms: DPRP2, 6430584G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224897
VEGA: 17
Homologene: 16385
Nfkb2
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
Synonyms: p52, NF kappaB2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18034
VEGA: 19
HGNC: HGNC:7795
Homologene: 1873
Prol1
Name: proline rich, lacrimal 1
Synonyms: Muc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17830
Sohlh2
Name: spermatogenesis and oogenesis specific basic helix-loop-helix 2
Synonyms: 4933406N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74434
Homologene: 9864
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Uspl1
Name: ubiquitin specific peptidase like 1
Synonyms: E430026A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231915
Homologene: 4235
Rint1
Name: RAD50 interactor 1
Synonyms: 2810450M21Rik, 1500019C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72772
Homologene: 11070
Tdrd12
Name: tudor domain containing 12
Synonyms: 2410070K17Rik, G1-476-14, ecat8, 2410004F06Rik, EG434165, repro23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71981
Homologene: 87053
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241116
Homologene: 28093
Inhbb
Name: inhibin beta-B
Synonyms: activin beta-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16324
HGNC: HGNC:6067
Homologene: 1654
Krt13
Name: keratin 13
Synonyms: Krt-1.13, K13, Krt1-13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16663
HGNC: HGNC:6415
Homologene: 40740
Gm973
Name: predicted gene 973
Synonyms: LOC381260
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381260
Homologene: 124277
Ccdc154
Name: coiled-coil domain containing 154
Synonyms: LOC207209, ntl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 207209
Homologene: 19361
Lztfl1
Name: leucine zipper transcription factor-like 1
Synonyms: 5530402H04Rik, 6130400H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 93730
HGNC: HGNC:6741
Homologene: 41368
Vmn1r4
Name: vomeronasal 1 receptor 4
Synonyms: C230065D10Rik, V1rc21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171194
Homologene: 131060
9230009I02Rik
Name: RIKEN cDNA 9230009I02 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 619293
Rdm1
Name: RAD52 motif 1
Synonyms: 2410008M22Rik, Rad52b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66599
Homologene: 11992
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Iglc2
Name: immunoglobulin lambda constant 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 110786
Rtkn2
Name: rhotekin 2
Synonyms: B130039D23Rik, RTKN2, Mbf, Plekhk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 170799
Homologene: 17065
Or10d5j
Name: olfactory receptor family 10 subfamily D member 5J
Synonyms: GA_x6K02T2PVTD-33657378-33656440, MOR224-10, Olfr976
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258364
VEGA: 9
Homologene: 64863
Stra8
Name: stimulated by retinoic acid gene 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20899
Homologene: 49197
Plekhd1os
Name: pleckstrin homology domain containing, family D (with coiled-coil domains) member 1, opposite strand
Synonyms: 0610009B14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68354
Gm4832
Name: predicted gene 4832
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 225058
Homologene: 136395
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 59,552,555 bp
  • G to A, chromosome 1 at 74,925,355 bp
  • C to G, chromosome 1 at 92,857,461 bp
  • T to A, chromosome 1 at 119,417,530 bp
  • C to T, chromosome 1 at 190,800,169 bp
  • A to G, chromosome 2 at 76,894,658 bp
  • A to G, chromosome 2 at 76,943,387 bp
  • T to C, chromosome 2 at 105,394,783 bp
  • G to A, chromosome 2 at 136,880,736 bp
  • T to A, chromosome 2 at 155,792,542 bp
  • T to C, chromosome 3 at 55,192,302 bp
  • A to T, chromosome 3 at 95,898,988 bp
  • T to C, chromosome 4 at 45,458,327 bp
  • A to G, chromosome 4 at 98,931,123 bp
  • T to C, chromosome 4 at 121,065,878 bp
  • T to G, chromosome 5 at 23,815,833 bp
  • A to T, chromosome 5 at 88,327,852 bp
  • C to T, chromosome 5 at 124,584,297 bp
  • G to A, chromosome 5 at 149,213,509 bp
  • A to T, chromosome 6 at 34,927,762 bp
  • A to G, chromosome 6 at 56,956,619 bp
  • A to G, chromosome 7 at 35,476,053 bp
  • A to T, chromosome 9 at 39,956,563 bp
  • A to T, chromosome 9 at 123,710,396 bp
  • T to G, chromosome 10 at 4,434,903 bp
  • T to A, chromosome 10 at 68,001,970 bp
  • T to C, chromosome 10 at 80,086,337 bp
  • T to C, chromosome 11 at 20,021,415 bp
  • A to T, chromosome 11 at 51,091,754 bp
  • A to G, chromosome 11 at 97,781,404 bp
  • C to T, chromosome 11 at 100,117,643 bp
  • C to T, chromosome 11 at 101,630,321 bp
  • C to T, chromosome 12 at 80,686,422 bp
  • T to A, chromosome 12 at 116,059,685 bp
  • A to G, chromosome 14 at 55,513,699 bp
  • T to C, chromosome 16 at 19,198,634 bp
  • T to C, chromosome 17 at 25,170,144 bp
  • C to T, chromosome 17 at 56,189,393 bp
  • T to G, chromosome 17 at 88,130,782 bp
  • T to G, chromosome 18 at 12,539,799 bp
  • A to G, chromosome 18 at 35,581,962 bp
  • A to G, chromosome 19 at 46,310,521 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5709 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043184-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.