Strain Name:
C57BL/6J-MtgxR5745Btlr/Mmmh
Stock Number:
043198-MU
Citation ID:
RRID:MMRRC_043198-MU
Other Names:
R5745 (G1), C57BL/6J-MtgxR5745Btlr
Major Collection:

Gene Information

Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, sema5, semaV, SemA, Semaa, LUCA-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20347
Homologene: 20972
Hmgn3
Name: high mobility group nucleosomal binding domain 3
Synonyms: HMGN3b, TRIP7, 1110002A15Rik, HMGN3a, 6330514M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 94353
Slc7a7
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 7
Synonyms: my+lat1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 20540
Homologene: 88701
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Rsf1
Name: remodeling and spacing factor 1
Synonyms: 4832420A03Rik, C030033M12Rik, XAP8, Hbxap, p325
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
3110082I17Rik
Name: RIKEN cDNA 3110082I17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73212
Homologene: 49901
Shoc2
Name: Shoc2, leucine rich repeat scaffold protein
Synonyms: Sur-8, soc-2 (suppressor of clear) homolog (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56392
VEGA: 19
Homologene: 7219
Tle3
Name: transducin-like enhancer of split 3
Synonyms: Grg3b, ESG, 2610103N05Rik, Grg3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 21887
Homologene: 21059
Pms1
Name: PMS1 homolog 1, mismatch repair system component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227099
HGNC: HGNC:9121
Homologene: 449
Copb2
Name: coatomer protein complex, subunit beta 2 (beta prime)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 50797
HGNC: HGNC:2232
Homologene: 3499
Dgcr8
Name: DGCR8, microprocessor complex subunit
Synonyms: Vo59c07, N41, D16H22S788E, D16H22S1742E, D16Wis2, DiGeorge syndrome critical region gene 8, Gy1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 94223
HGNC: HGNC:2847
Homologene: 11223
Smcr8
Name: Smith-Magenis syndrome chromosome region, candidate 8 homolog (human)
Synonyms: 2310076G09Rik, D030073L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237782
Homologene: 16989
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269700
Homologene: 28297
Kel
Name: Kell blood group
Synonyms: CD238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23925
HGNC: HGNC:6308
Homologene: 362
Dock8
Name: dedicator of cytokinesis 8
Synonyms: 1200017A24Rik, A130095G14Rik, 5830472H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 76088
VEGA: 19
Homologene: 52414
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Vmn2r57
Name: vomeronasal 2, receptor 57
Synonyms: EG269902
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269902
Homologene: 104832
Fer1l6
Name: fer-1-like 6 (C. elegans)
Synonyms: EG631797
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 631797
Homologene: 53396
Intu
Name: inturned planar cell polarity protein
Synonyms: 9230116I04Rik, 9430087H23Rik, Pdzd6, Pdzk6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 380614
Homologene: 41059
Adcy8
Name: adenylate cyclase 8
Synonyms: AC8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11514
VEGA: 15
HGNC: HGNC:239
Homologene: 37443
Nrp1
Name: neuropilin 1
Synonyms: NPN-1, Neuropilin-1, Npn1, NP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18186
HGNC: HGNC:8004
Homologene: 2876
Myom2
Name: myomesin 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17930
HGNC: HGNC:7614
Homologene: 2953
Tmem94
Name: transmembrane protein 94
Synonyms: 2310067B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71947
Homologene: 8831
Ephb1
Name: Eph receptor B1
Synonyms: Elkh, C130099E04Rik, Elk, Hek6, Net, Cek6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270190
HGNC: HGNC:3392
Homologene: 20936
Fpr1
Name: formyl peptide receptor 1
Synonyms: fMLF-R, FPR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14293
HGNC: HGNC:3826
Homologene: 20466
4933407L21Rik
Name: RIKEN cDNA 4933407L21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71141
Cobll1
Name: Cobl-like 1
Synonyms: D430044D16Rik, Coblr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 319876
Homologene: 8933
Vmn2r45
Name: vomeronasal 2, receptor 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100042810
Homologene: 113703
Olfr787
Name: olfactory receptor 787
Synonyms: MOR111-10, GA_x6K02T2PULF-11141498-11142436
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258069
Homologene: 105326
Olfr958
Name: olfactory receptor 958
Synonyms: MOR224-9, GA_x6K02T2PVTD-33247839-33246901
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258327
VEGA: 9
Homologene: 27128
Pcsk1
Name: proprotein convertase subtilisin/kexin type 1
Synonyms: Nec1, Nec-1, Phpp-1, PC3, prohormone convertase 1/3, SPC3, PC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18548
HGNC: HGNC:8743
Homologene: 379
Cpa5
Name: carboxypeptidase A5
Synonyms: 4930430M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74649
Homologene: 62246
Ighv3-4
Name: immunoglobulin heavy variable V3-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629833
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 53,207,702 bp
  • T to G, chromosome 1 at 85,931,274 bp
  • T to C, chromosome 2 at 65,098,457 bp
  • A to G, chromosome 3 at 40,692,972 bp
  • G to A, chromosome 5 at 121,353,502 bp
  • G to A, chromosome 5 at 139,364,073 bp
  • T to A, chromosome 6 at 30,630,437 bp
  • C to T, chromosome 6 at 41,699,027 bp
  • T to A, chromosome 7 at 8,483,075 bp
  • A to T, chromosome 7 at 41,448,471 bp
  • GGGCCAGG to GGG, chromosome 7 at 80,098,342 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,920 bp
  • T to A, chromosome 8 at 15,122,705 bp
  • A to T, chromosome 8 at 128,468,448 bp
  • T to C, chromosome 9 at 39,550,691 bp
  • C to A, chromosome 9 at 61,414,851 bp
  • T to A, chromosome 9 at 83,112,229 bp
  • T to C, chromosome 9 at 98,574,111 bp
  • C to T, chromosome 9 at 102,195,434 bp
  • C to A, chromosome 9 at 107,601,429 bp
  • T to A, chromosome 10 at 129,463,438 bp
  • A to T, chromosome 11 at 60,784,151 bp
  • T to A, chromosome 11 at 115,777,335 bp
  • T to A, chromosome 12 at 114,253,768 bp
  • A to C, chromosome 13 at 75,131,960 bp
  • G to A, chromosome 14 at 54,377,835 bp
  • A to C, chromosome 14 at 103,156,453 bp
  • A to G, chromosome 15 at 58,571,389 bp
  • A to T, chromosome 15 at 64,920,471 bp
  • A to T, chromosome 16 at 18,280,443 bp
  • A to G, chromosome 17 at 17,877,082 bp
  • T to A, chromosome 19 at 25,130,397 bp
  • C to A, chromosome 19 at 54,029,892 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5745 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043198-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.