Strain Name:
C57BL/6J-MtgxR5746Btlr/Mmmh
Stock Number:
043199-MU
Citation ID:
RRID:MMRRC_043199-MU
Other Names:
R5746 (G1), C57BL/6J-MtgxR5746Btlr
Major Collection:

Strain Information

Ptpn14
Name: protein tyrosine phosphatase, non-receptor type 14
Synonyms: PTP36, C130080N23Rik, OTTMUSG00000022087
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19250
HGNC: HGNC:9647
Homologene: 3941
Cbln3
Name: cerebellin 3 precursor protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 56410
VEGA: 14
Homologene: 56861
Kdm2b
Name: lysine (K)-specific demethylase 2B
Synonyms: Cxxc2, Jhdm1b, Fbxl10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 30841
Homologene: 13069
Stx16
Name: syntaxin 16
Synonyms: 5430410K23Rik, SYN16, 6330500A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228960
Homologene: 2791
Smg6
Name: SMG6 nonsense mediated mRNA decay factor
Synonyms: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103677
Homologene: 23024
Tbc1d15
Name: TBC1 domain family, member 15
Synonyms: 4432405K22Rik, Ly6dl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66687
VEGA: 10
Homologene: 11249
Zfp146
Name: zinc finger protein 146
Synonyms: OZF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26465
Homologene: 117615
Glb1l2
Name: galactosidase, beta 1-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244757
Homologene: 71790
Kel
Name: Kell blood group
Synonyms: CD238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 23925
HGNC: HGNC:6308
Homologene: 362
Ank1
Name: ankyrin 1, erythroid
Synonyms: Ank-1, pale
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Zcchc14
Name: zinc finger, CCHC domain containing 14
Synonyms: Bdg29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 142682
Homologene: 9037
Cpsf1
Name: cleavage and polyadenylation specific factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 94230
VEGA: 15
HGNC: HGNC:2324
Homologene: 40865
Igkv6-29
Name: immunoglobulin kappa chain variable 6-29
Synonyms: Gm16692
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 620191
Gvin3
Name: GTPase, very large interferon inducible, family member 3
Synonyms: Gm1966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434223
Homologene: 32708
Ttf1
Name: transcription termination factor, RNA polymerase I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22130
Homologene: 137223
Cdh12
Name: cadherin 12
Synonyms: Br-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 215654
HGNC: HGNC:1751
Homologene: 37873
Kcnma1
Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
Synonyms: Slo, mSlo1, Slo1, MaxiK, BK channel alpha subunit, 5730414M22Rik, BKCa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16531
HGNC: HGNC:6284
Homologene: 1693
Plekha6
Name: pleckstrin homology domain containing, family A member 6
Synonyms: Pepp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240753
Homologene: 135779
Cyp2d10
Name: cytochrome P450, family 2, subfamily d, polypeptide 10
Synonyms: P450-2D, Cyp2d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13101
VEGA: 15
HGNC: HGNC:2625
Homologene: 86099
Adora3
Name: adenosine A3 receptor
Synonyms: A3AR, ARA3, Gpcr 2, 4930578J19Rik, AA3R, A3R, 1700001D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11542
Homologene: 550
Vmn2r11
Name: vomeronasal 2, receptor 11
Synonyms: EG384219
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384219
Homologene: 129606
Ankrd16
Name: ankyrin repeat domain 16
Synonyms: D430029B21Rik, 2810455F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320816
Homologene: 19509
Nod2
Name: nucleotide-binding oligomerization domain containing 2
Synonyms: F830032C23Rik, Card15, Nlrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 257632
HGNC: HGNC:5331
Homologene: 11156
Gba2
Name: glucosidase beta 2
Synonyms: bile acid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230101
Homologene: 10859
Atp6ap1l
Name: ATPase, H+ transporting, lysosomal accessory protein 1-like
Synonyms: EG435376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 435376
Homologene: 28130
Rps10-ps1
Name: ribosomal protein S10, pseudogene 1
Synonyms: Gm9180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 668457
Gm11149
Name: predicted gene 11149
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 100036537
VEGA: 9
Tpm2
Name: tropomyosin 2, beta
Synonyms: Tpm-2, Trop-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22004
Homologene: 134309
Ly6g
Name: lymphocyte antigen 6 complex, locus G
Synonyms: Gr1, Ly-6G, Gr-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 546644
Homologene: 113718
Gm6866
Name: predicted gene 6866
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 628304
Homologene: 80005
Zfp994
Name: zinc finger protein 994
Synonyms: Gm4944
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240038
VEGA: 17
Homologene: 133246
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 133,272,307 bp
  • G to A, chromosome 1 at 189,846,413 bp
  • T to A, chromosome 2 at 11,784,367 bp
  • G to T, chromosome 2 at 29,065,742 bp
  • G to A, chromosome 2 at 174,093,499 bp
  • A to G, chromosome 3 at 95,622,699 bp
  • A to G, chromosome 3 at 105,907,810 bp
  • A to G, chromosome 4 at 43,519,731 bp
  • T to C, chromosome 4 at 43,568,465 bp
  • T to A, chromosome 5 at 109,053,694 bp
  • A to G, chromosome 5 at 122,879,364 bp
  • C to T, chromosome 6 at 41,699,027 bp
  • C to A, chromosome 6 at 70,138,600 bp
  • T to C, chromosome 7 at 30,162,393 bp
  • A to T, chromosome 7 at 41,217,667 bp
  • T to C, chromosome 7 at 106,598,329 bp
  • C to T, chromosome 8 at 23,116,596 bp
  • T to C, chromosome 8 at 88,664,342 bp
  • T to C, chromosome 8 at 121,604,639 bp
  • G to A, chromosome 9 at 26,796,790 bp
  • C to T, chromosome 9 at 49,546,194 bp
  • T to C, chromosome 10 at 115,210,279 bp
  • G to A, chromosome 11 at 75,139,287 bp
  • T to C, chromosome 13 at 90,883,579 bp
  • T to C, chromosome 14 at 23,494,567 bp
  • A to G, chromosome 14 at 55,883,144 bp
  • T to A, chromosome 15 at 21,358,724 bp
  • A to T, chromosome 15 at 75,156,747 bp
  • C to T, chromosome 15 at 76,599,837 bp
  • T to G, chromosome 15 at 82,405,271 bp
  • A to G, chromosome 17 at 22,201,273 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5746 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043199-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.