Strain Name:
C57BL/6J-MtgxR5827Btlr/Mmmh
Stock Number:
043218-MU
Citation ID:
RRID:MMRRC_043218-MU
Other Names:
R5827 (G1), C57BL/6J-MtgxR5827Btlr
Major Collection:

Gene Information

Tnpo1
Name: transportin 1
Synonyms: D13Ertd688e, Kpnb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 238799
HGNC: HGNC:6401
Homologene: 5358
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Mycn
Name: v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
Synonyms: Nmyc1, N-myc, Nmyc-1, Nmyc, bHLHe37
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 18109
HGNC: HGNC:7559
Homologene: 3922
Mapk8
Name: mitogen-activated protein kinase 8
Synonyms: JNK1, c-Jun N-terminal kinase, Prkm8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 26419
HGNC: HGNC:6881
Homologene: 56760
Nup188
Name: nucleoporin 188
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227699
Homologene: 45683
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 9030205D12Rik, 1810014J18Rik, AD013, A330063D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 109151
Homologene: 11844
Klhl24
Name: kelch-like 24
Synonyms: 4930429H24Rik, 6530402O20Rik, 1110046J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 75785
VEGA: 16
Homologene: 32352
Pcsk9
Name: proprotein convertase subtilisin/kexin type 9
Synonyms: Narc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100102
Homologene: 17790
Npnt
Name: nephronectin
Synonyms: 1110009H02Rik, POEM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 114249
Homologene: 14138
Tdp2
Name: tyrosyl-DNA phosphodiesterase 2
Synonyms: Ttrap, D13Ertd656e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 56196
Homologene: 9591
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 319262
Homologene: 14127
Fbxw4
Name: F-box and WD-40 domain protein 4
Synonyms: dactylin, Fbw4, dactylyn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 30838
Homologene: 32197
Ube2cbp
Name: ubiquitin-conjugating enzyme E2C binding protein
Synonyms: 2610018I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 70348
Homologene: 18898
Sec63
Name: SEC63-like (S. cerevisiae)
Synonyms: 5730478J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 140740
Homologene: 5220
Agl
Name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase
Synonyms: 1110061O17Rik, 9630046L06Rik, 9430004C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 77559
HGNC: HGNC:321
Homologene: 536
Dscam
Name: DS cell adhesion molecule
Synonyms: 4932410A21Rik, Down syndrome cell adhesion molecule
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 13508
HGNC: HGNC:3039
Homologene: 74393
Slx4
Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
Synonyms: D16Bwg1016e, Btbd12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 52864
Homologene: 23770
Nek11
Name: NIMA (never in mitosis gene a)-related expressed kinase 11
Synonyms: 4932416N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 208583
Homologene: 57008
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Skint10
Name: selection and upkeep of intraepithelial T cells 10
Synonyms: A030001H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230613
Homologene: 136292
Col6a5
Name: collagen, type VI, alpha 5
Synonyms: Gm7455, Col6a5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 665033
Homologene: 122792
Sh3pxd2b
Name: SH3 and PX domains 2B
Synonyms: Fad49, Tsk4, G431001E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268396
Homologene: 27952
Olfr1277
Name: olfactory receptor 1277
Synonyms: GA_x6K02T2Q125-72321818-72320907, MOR248-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258391
Homologene: 105176
Pcdhb9
Name: protocadherin beta 9
Synonyms: Pcdhb4C, PcdhbI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93880
Homologene: 87124
Serpina3b
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3B
Synonyms: A030003A19Rik, antitrypsin, alpha-1 antiproteinase, 6A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 271047
HGNC: HGNC:16
Homologene: 120222
Hist1h1t
Name: histone cluster 1, H1t
Synonyms: H1ft
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 107970
HGNC: HGNC:4720
Homologene: 3889
Nr1d2
Name: nuclear receptor subfamily 1, group D, member 2
Synonyms: RVR, Rev-erb beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 353187
HGNC: HGNC:7963
Homologene: 3763
Pcdhb7
Name: protocadherin beta 7
Synonyms: PcdhbG, Pcdhb4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93878
Homologene: 87123
Tiam2
Name: T cell lymphoma invasion and metastasis 2
Synonyms: STEF, 3000002F19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 24001
VEGA: 17
Homologene: 40796
Disc1
Name: disrupted in schizophrenia 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 244667
HGNC: HGNC:2888
Homologene: 10257
P2rx2
Name: purinergic receptor P2X, ligand-gated ion channel, 2
Synonyms: P2X2a, P2x2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231602
Homologene: 14251
Zfp58
Name: zinc finger protein 58
Synonyms: Mfg-1, Zfp817, A530094I17Rik, Mfg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 238693
Rhebl1
Name: Ras homolog enriched in brain like 1
Synonyms: 1810036J22Rik, Rheb2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 69159
Homologene: 5477
Olfr957
Name: olfactory receptor 957
Synonyms: GA_x6K02T2PVTD-33208209-33207274, MOR171-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258740
VEGA: 9
Ugt1a2
Name: UDP glucuronosyltransferase 1 family, polypeptide A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22236
Homologene: 86211
Cavin4
Name: caveolae associated 4
Synonyms: cavin 4, 2310039E09Rik, Murc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 68016
Homologene: 12224
Gstm6
Name: glutathione S-transferase, mu 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 14867
Homologene: 117950
Ptgr2
Name: prostaglandin reductase 2
Synonyms: 9630002F03Rik, Zadh1, PGR-2, 1810016I24Rik, B830026H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 77219
Homologene: 39824
Olfr1029
Name: olfactory receptor 1029
Synonyms: GA_x6K02T2Q125-47454152-47455126, MOR198-1P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258154
Homologene: 73972
Olfr164
Name: olfactory receptor 164
Synonyms: MOR279-2, GA_x54KRFPKG5P-15738260-15737319
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 258443
HGNC: HGNC:8269
Homologene: 51725
Tmem200c
Name: transmembrane protein 200C
Synonyms: Gm6338
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 622645
VEGA: 17
Homologene: 78765
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 88,201,065 bp
  • C to T, chromosome 2 at 30,339,847 bp
  • C to A, chromosome 2 at 85,975,306 bp
  • A to T, chromosome 2 at 111,269,921 bp
  • G to A, chromosome 3 at 98,072,862 bp
  • G to A, chromosome 3 at 107,942,236 bp
  • T to C, chromosome 3 at 116,781,054 bp
  • A to C, chromosome 3 at 132,906,775 bp
  • A to T, chromosome 4 at 48,672,074 bp
  • C to T, chromosome 4 at 106,448,947 bp
  • T to C, chromosome 4 at 112,746,775 bp
  • C to T, chromosome 5 at 110,340,329 bp
  • A to G, chromosome 8 at 90,989,450 bp
  • T to C, chromosome 8 at 125,135,365 bp
  • T to A, chromosome 9 at 39,511,058 bp
  • T to C, chromosome 9 at 86,372,436 bp
  • T to C, chromosome 9 at 105,314,745 bp
  • G to A, chromosome 9 at 105,928,120 bp
  • A to G, chromosome 10 at 42,827,912 bp
  • A to G, chromosome 11 at 32,422,422 bp
  • C to A, chromosome 11 at 109,977,813 bp
  • G to A, chromosome 12 at 12,939,793 bp
  • G to A, chromosome 12 at 84,295,336 bp
  • A to G, chromosome 12 at 104,130,777 bp
  • A to G, chromosome 13 at 23,696,202 bp
  • T to C, chromosome 13 at 24,831,853 bp
  • A to T, chromosome 13 at 67,491,293 bp
  • T to C, chromosome 13 at 98,856,908 bp
  • C to A, chromosome 14 at 18,222,248 bp
  • G to A, chromosome 14 at 33,408,093 bp
  • T to A, chromosome 15 at 98,878,270 bp
  • G to T, chromosome 16 at 4,001,284 bp
  • T to A, chromosome 16 at 19,286,432 bp
  • T to A, chromosome 16 at 20,120,121 bp
  • A to G, chromosome 16 at 96,649,991 bp
  • A to G, chromosome 17 at 3,448,489 bp
  • A to G, chromosome 17 at 68,842,009 bp
  • A to G, chromosome 18 at 37,342,024 bp
  • A to T, chromosome 18 at 37,401,958 bp
  • C to T, chromosome 18 at 37,959,873 bp
  • T to C, chromosome 19 at 45,579,657 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5827 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043218-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.