Strain Name:
C57BL/6J-MtgxR6003Btlr/Mmmh
Stock Number:
043252-MU
Citation ID:
RRID:MMRRC_043252-MU
Other Names:
R6003 (G1), C57BL/6J-MtgxR6003Btlr
Major Collection:

Strain Information

Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, neurexin II alpha, 6430591O13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Kdm4b
Name: lysine (K)-specific demethylase 4B
Synonyms: 4732474L06Rik, Jmjd2b
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 193796
Homologene: 27773
Usp19
Name: ubiquitin specific peptidase 19
Synonyms: 8430421I07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71472
Homologene: 41730
Nup205
Name: nucleoporin 205
Synonyms: 3830404O05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70699
Homologene: 45971
Nup133
Name: nucleoporin 133
Synonyms: 4832420O05Rik, mermaid
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234865
Homologene: 32402
Eif3a
Name: eukaryotic translation initiation factor 3, subunit A
Synonyms: Csma, Eif3, A830012B05Rik, Eif3s10
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13669
VEGA: 19
HGNC: HGNC:3271
Homologene: 2779
E2f8
Name: E2F transcription factor 8
Synonyms: 4432406C08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108961
Homologene: 11654
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 195018
Homologene: 9027
Ccdc3
Name: coiled-coil domain containing 3
Synonyms: 2310045O21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74186
Homologene: 12870
Gfpt1
Name: glutamine fructose-6-phosphate transaminase 1
Synonyms: GFAT, GFA, GFAT1, 2810423A18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14583
HGNC: HGNC:4241
Homologene: 68220
Srebf1
Name: sterol regulatory element binding transcription factor 1
Synonyms: SREBP-1, ADD-1, SREBP-1c, SREBP-1a, SREBP1, SREBP1c, bHLHd1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20787
Homologene: 3079
Ncoa2
Name: nuclear receptor coactivator 2
Synonyms: TIF2/GRIP-1, Grip1, D1Ertd433e, TIF2, glucocorticoid receptor-interacting protein 1, TIF-2, SRC-2, KAT13C, bHLHe75
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17978
HGNC: HGNC:7669
Homologene: 4768
Ints2
Name: integrator complex subunit 2
Synonyms: 2810417D08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70422
Homologene: 10801
Nup54
Name: nucleoporin 54
Synonyms: 3110079L04Rik, 54kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269113
Homologene: 41169
Cachd1
Name: cache domain containing 1
Synonyms: 1190007F10Rik, Vwcd1, B430218L07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320508
Homologene: 10854
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
A4galt
Name: alpha 1,4-galactosyltransferase
Synonyms: Gb3 synthase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239559
VEGA: 15
Homologene: 9690
Gucy1b1
Name: guanylate cyclase 1, soluble, beta 1
Synonyms: beta 1 sGC, Gucy1b3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54195
HGNC: HGNC:4687
Homologene: 664
Abcb11
Name: ATP-binding cassette, sub-family B member 11
Synonyms: PFIC2, ABC16, PGY4, Lith1, sister of P-glycoprotein, Bsep
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27413
HGNC: HGNC:42
Homologene: 74509
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319695
Homologene: 85163
Slc15a2
Name: solute carrier family 15 (H+/peptide transporter), member 2
Synonyms: Pept2, 8430408C16Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 57738
Homologene: 56912
Rfx6
Name: regulatory factor X, 6
Synonyms: 4930572O07Rik, Rfxdc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320995
Homologene: 18318
Rpap2
Name: RNA polymerase II associated protein 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231571
Homologene: 11733
Rskr
Name: ribosomal protein S6 kinase related
Synonyms: BC030499
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216976
Homologene: 79669
Pappa2
Name: pappalysin 2
Synonyms: placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E, PAPP-A2, PLAC3, Pappe
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23850
Homologene: 10661
Parpbp
Name: PARP1 binding protein
Synonyms: 4930547N16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 75317
Homologene: 49519
Vmn1r86
Name: vomeronasal 1 receptor 86
Synonyms: Gm10301
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100312473
Homologene: 74345
Ggps1
Name: geranylgeranyl diphosphate synthase 1
Synonyms: GGPP synthase, 9530089B04Rik, 1810026C22Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14593
VEGA: 13
HGNC: HGNC:4249
Homologene: 31267
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627479
Homologene: 129606
Hoxc9
Name: homeobox C9
Synonyms: Hox-3.2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 15427
HGNC: HGNC:5130
Homologene: 130558
Ap1m1
Name: adaptor-related protein complex AP-1, mu subunit 1
Synonyms: AP47, mu1A, [m]1A, Adtm1A, Cltnm
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11767
Homologene: 4017
Vps52
Name: VPS52 GARP complex subunit
Synonyms: ARE1, tcl-w5, D430041K17Rik, Sacm2l, tclw5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224705
Homologene: 5756
Lax1
Name: lymphocyte transmembrane adaptor 1
Synonyms: E430019B13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240754
Homologene: 49504
Tmem214
Name: transmembrane protein 214
Synonyms: 4921530J21Rik, 1110039B18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68796
Homologene: 9801
Cope
Name: coatomer protein complex, subunit epsilon
Synonyms: Cope1, 1110005D17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 59042
HGNC: HGNC:2234
Homologene: 5254
Or2ak5
Name: olfactory receptor family 2 subfamily AK member 5
Synonyms: GA_x6K02T2NKPP-692816-693736, MOR285-1, Olfr318
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258494
Homologene: 133825
Antxrl
Name: anthrax toxin receptor-like
Synonyms: 1700112N15Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239029
VEGA: 14
Homologene: 52111
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104816
VEGA: 12
Homologene: 113390
Or5b3
Name: olfactory receptor family 5 subfamily B member 3
Synonyms: GA_x6K02T2RE5P-3743369-3744289, MOR202-11, Olfr1469
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258690
HGNC: HGNC:8324
Homologene: 133675
Marveld3
Name: MARVEL (membrane-associating) domain containing 3
Synonyms: MARVD3, 1810006A16Rik, Mrvldc3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 73608
Homologene: 12504
Gtf2a1l
Name: general transcription factor IIA, 1-like
Synonyms: 1700011N16Rik, Alf, Gtf2a1lf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71828
Homologene: 86732
Obp2a
Name: odorant binding protein 2A
Synonyms: Lcn13
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227627
Homologene: 136130
Rdh16f2
Name: RDH16 family member 2
Synonyms: BC089597
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216454
Homologene: 129514
As3mt
Name: arsenite methyltransferase
Synonyms: Cyt19, 2310045H08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 57344
VEGA: 19
Homologene: 10754
Cnpy1
Name: canopy FGF signaling regulator 1
Synonyms: 9630008K15Rik, 1500012D20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269637
Homologene: 89678
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,167,030 bp
  • T to A, chromosome 1 at 72,698,887 bp
  • T to A, chromosome 1 at 133,684,096 bp
  • T to C, chromosome 1 at 158,936,250 bp
  • T to C, chromosome 2 at 5,141,407 bp
  • A to T, chromosome 2 at 25,701,139 bp
  • T to C, chromosome 2 at 69,243,467 bp
  • C to A, chromosome 3 at 82,058,277 bp
  • A to G, chromosome 3 at 88,896,093 bp
  • T to C, chromosome 4 at 100,952,019 bp
  • T to C, chromosome 5 at 28,245,761 bp
  • C to A, chromosome 5 at 30,870,724 bp
  • A to T, chromosome 5 at 92,422,994 bp
  • A to G, chromosome 5 at 107,601,901 bp
  • A to T, chromosome 5 at 108,797,382 bp
  • T to C, chromosome 6 at 35,212,816 bp
  • T to A, chromosome 6 at 87,088,248 bp
  • C to T, chromosome 7 at 13,102,198 bp
  • T to C, chromosome 7 at 48,870,777 bp
  • AGGGCCAGGG to AGG, chromosome 7 at 80,098,341 bp
  • T to C, chromosome 8 at 70,304,635 bp
  • A to G, chromosome 8 at 72,249,167 bp
  • A to T, chromosome 8 at 109,954,328 bp
  • A to T, chromosome 8 at 123,938,292 bp
  • T to C, chromosome 9 at 108,496,380 bp
  • C to T, chromosome 10 at 51,708,587 bp
  • A to G, chromosome 10 at 88,133,158 bp
  • A to T, chromosome 10 at 127,876,332 bp
  • A to T, chromosome 11 at 58,720,370 bp
  • T to C, chromosome 11 at 60,207,104 bp
  • T to A, chromosome 11 at 72,824,065 bp
  • T to G, chromosome 11 at 78,293,020 bp
  • T to C, chromosome 11 at 86,238,468 bp
  • T to C, chromosome 12 at 112,113,042 bp
  • T to G, chromosome 13 at 14,054,002 bp
  • G to T, chromosome 14 at 34,075,635 bp
  • T to C, chromosome 15 at 83,228,111 bp
  • T to C, chromosome 15 at 102,981,879 bp
  • T to C, chromosome 16 at 36,754,548 bp
  • T to A, chromosome 17 at 33,956,094 bp
  • C to T, chromosome 17 at 56,396,916 bp
  • G to T, chromosome 17 at 88,694,103 bp
  • C to A, chromosome 19 at 6,498,328 bp
  • T to C, chromosome 19 at 13,411,039 bp
  • C to T, chromosome 19 at 46,708,128 bp
  • A to T, chromosome 19 at 60,766,881 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6003 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043252-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.