Strain Name:
C57BL/6J-MtgxR6016Btlr/Mmmh
Stock Number:
043255-MU
Citation ID:
RRID:MMRRC_043255-MU
Other Names:
R6016 (G1), C57BL/6J-MtgxR6016Btlr
Major Collection:

Gene Information

Rprd2
Name: regulation of nuclear pre-mRNA domain containing 2
Synonyms: 6720469I21Rik, 4930535B03Rik, 2810036A19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 75137
Homologene: 19997
Ncapg2
Name: non-SMC condensin II complex, subunit G2
Synonyms: 5830426I05Rik, Luzp5, Mtb, mCAP-G2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 76044
VEGA: 12
Homologene: 9820
Slitrk6
Name: SLIT and NTRK-like family, member 6
Synonyms: 4832410J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 239250
VEGA: 14
Homologene: 12986
Nop56
Name: NOP56 ribonucleoprotein
Synonyms: Nol5a, 56kDa with KKE/D repeat, NOP56, 2310044F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67134
Homologene: 4660
Plxnb2
Name: plexin B2
Synonyms: 1110007H23Rik, Debt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 140570
HGNC: HGNC:9104
Homologene: 66630
Casz1
Name: castor zinc finger 1
Synonyms: Cst, 2410019P08Rik, D4Ertd432e, castor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69743
Homologene: 9824
Pes1
Name: pescadillo ribosomal biogenesis factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 64934
HGNC: HGNC:8848
Homologene: 5984
Tnks1bp1
Name: tankyrase 1 binding protein 1
Synonyms: TAB182
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228140
Homologene: 14117
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: b2b1537Clo, b2b1565Clo, b2b3491Clo, b2b1154Clo, Mdnah5, Dnahc5, b2b1134Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Gm17067
Name: predicted gene 17067
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 101056115
Sptbn5
Name: spectrin beta, non-erythrocytic 5
Synonyms: Spnb5, EG640524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 640524
Homologene: 41150
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 192187
Homologene: 9035
Dcaf12
Name: DDB1 and CUL4 associated factor 12
Synonyms: 5830424K06Rik, Wdr40a, 1500001L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 68970
Homologene: 49418
Gne
Name: glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Synonyms: 2310066H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 50798
Homologene: 3996
Shkbp1
Name: Sh3kbp1 binding protein 1
Synonyms: SB1, B930062H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 192192
Homologene: 26700
Pde1a
Name: phosphodiesterase 1A, calmodulin-dependent
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 18573
HGNC: HGNC:8774
Homologene: 21043
Olfr1344
Name: olfactory receptor 1344
Synonyms: MOR124-1, GA_x6K02T2QGBW-3169916-3170881, 4930415J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 257882
Olfr467
Name: olfactory receptor 467
Synonyms: MOR204-33P, GA_x6K02T2PBJ9-10144091-10145011
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 257919
Homologene: 81542
Psg23
Name: pregnancy-specific glycoprotein 23
Synonyms: 1620401C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56868
Homologene: 110989
Tgm6
Name: transglutaminase 6
Synonyms: TGM3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241636
Homologene: 27970
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 620697
Homologene: 115466
Hs3st2
Name: heparan sulfate (glucosamine) 3-O-sulfotransferase 2
Synonyms: 6430516N12Rik, A830061E14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 195646
HGNC: HGNC:5195
Homologene: 21220
Gsdmc3
Name: gasdermin C3
Synonyms: 9930109F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 270328
HGNC: HGNC:7151
Homologene: 69487
Lats2
Name: large tumor suppressor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 50523
HGNC: HGNC:6515
Homologene: 56678
Olfr761
Name: olfactory receptor 761
Synonyms: MOR218-6P, MOR218-6P, Olfr1552-ps1, MOR218-12, MOR218-5P, GA_x6K02T2PSCP-2403971-2403000
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 258094
Homologene: 134080
Slc38a8
Name: solute carrier family 38, member 8
Synonyms: LOC234788
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234788
Homologene: 19028
Entpd2
Name: ectonucleoside triphosphate diphosphohydrolase 2
Synonyms: Cd39l1, NTPDase2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 12496
HGNC: HGNC:3364
Homologene: 20333
Il19
Name: interleukin 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329244
HGNC: HGNC:5990
Homologene: 17813
Mill2
Name: MHC I like leukocyte 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243864
HGNC: HGNC:7091
Homologene: 88329
Cltb
Name: clathrin, light polypeptide (Lcb)
Synonyms: 2310046E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 74325
HGNC: HGNC:2091
Homologene: 37532
Apol9b
Name: apolipoprotein L 9b
Synonyms: 2310016F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 71898
VEGA: 15
Homologene: 77567
Vmn1r157
Name: vomeronasal 1 receptor 157
Synonyms: Gm8699, Vmn1r109, Gm4517
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 667551
Homologene: 104166
Gm3250
Name: predicted gene 3250
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 100041281
VEGA: 10
Homologene: 115744
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 130,935,981 bp
  • C to T, chromosome 2 at 25,398,556 bp
  • T to C, chromosome 2 at 79,865,062 bp
  • T to C, chromosome 2 at 85,052,390 bp
  • G to T, chromosome 2 at 120,050,092 bp
  • A to G, chromosome 2 at 130,141,228 bp
  • T to C, chromosome 2 at 130,276,625 bp
  • A to G, chromosome 3 at 95,787,373 bp
  • A to G, chromosome 4 at 41,313,267 bp
  • T to C, chromosome 4 at 44,039,063 bp
  • C to A, chromosome 4 at 148,934,584 bp
  • A to T, chromosome 7 at 6,440,614 bp
  • G to T, chromosome 7 at 18,612,187 bp
  • G to T, chromosome 7 at 18,856,448 bp
  • A to G, chromosome 7 at 22,761,847 bp
  • A to G, chromosome 7 at 27,354,401 bp
  • G to T, chromosome 7 at 42,708,230 bp
  • A to T, chromosome 7 at 85,222,245 bp
  • A to T, chromosome 7 at 107,815,012 bp
  • A to G, chromosome 7 at 121,500,699 bp
  • A to T, chromosome 8 at 119,494,305 bp
  • A to G, chromosome 10 at 77,782,533 bp
  • T to A, chromosome 11 at 3,978,004 bp
  • G to A, chromosome 12 at 116,426,607 bp
  • T to C, chromosome 13 at 54,606,667 bp
  • A to T, chromosome 14 at 31,158,993 bp
  • A to T, chromosome 14 at 57,734,175 bp
  • A to G, chromosome 14 at 110,750,526 bp
  • T to C, chromosome 15 at 28,327,884 bp
  • T to G, chromosome 15 at 63,868,412 bp
  • T to C, chromosome 15 at 77,735,858 bp
  • T to A, chromosome 15 at 89,161,022 bp
  • A to T, chromosome 17 at 37,952,076 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6016 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043255-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.