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Strain Name:
C57BL/6J-MtgxR5135Btlr/Mmmh
Stock Number:
043261-MU
Citation ID:
RRID:MMRRC_043261-MU
Other Names:
R5135 (G1)
Major Collection:

Strain Information

Kitl
Name: kit ligand
Synonyms: SF, stem cell factor, Steel factor, SLF, Mgf, Sl, Gb, grizzle-belly, Steel, SCF, Kitlg, blz
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17311
HGNC: HGNC:6343
Homologene: 692
Zfa-ps
Name: zinc finger protein, autosomal, pseudogene
Synonyms: Zfa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22639
Zic4
Name: zinc finger protein of the cerebellum 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22774
Homologene: 32075
Gcm2
Name: glial cells missing homolog 2
Synonyms: Gcm1-rs2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107889
VEGA: 13
HGNC: HGNC:4198
Homologene: 3490
Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Kpna4
Name: karyopherin subunit alpha 4
Synonyms: IPOA3, 1110058D08Rik, importin alpha 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16649
HGNC: HGNC:6397
Homologene: 20521
Slc4a2
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: B3RP, Ae2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20535
Homologene: 128699
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 133,633,789 bp
  • T to A, chromosome 1 at 134,588,746 bp
  • T to A, chromosome 1 at 161,198,231 bp
  • T to C, chromosome 1 at 166,483,982 bp
  • T to C, chromosome 1 at 184,033,506 bp
  • A to G, chromosome 2 at 28,559,423 bp
  • A to T, chromosome 2 at 89,751,895 bp
  • A to T, chromosome 2 at 150,255,490 bp
  • A to T, chromosome 2 at 165,353,670 bp
  • T to A, chromosome 2 at 180,202,220 bp
  • A to T, chromosome 3 at 30,684,890 bp
  • G to T, chromosome 3 at 32,955,831 bp
  • T to C, chromosome 3 at 38,455,803 bp
  • C to T, chromosome 3 at 69,092,809 bp
  • T to C, chromosome 3 at 95,585,220 bp
  • T to C, chromosome 3 at 108,398,659 bp
  • C to T, chromosome 3 at 142,304,365 bp
  • A to T, chromosome 3 at 144,954,946 bp
  • G to T, chromosome 4 at 57,855,912 bp
  • G to T, chromosome 4 at 88,868,271 bp
  • A to G, chromosome 4 at 115,489,960 bp
  • G to T, chromosome 4 at 126,720,544 bp
  • T to G, chromosome 4 at 143,419,009 bp
  • T to A, chromosome 4 at 154,882,324 bp
  • G to A, chromosome 5 at 24,430,127 bp
  • T to C, chromosome 5 at 92,303,934 bp
  • T to A, chromosome 5 at 110,101,363 bp
  • C to T, chromosome 5 at 151,062,767 bp
  • A to T, chromosome 6 at 68,755,554 bp
  • T to C, chromosome 6 at 91,773,916 bp
  • A to G, chromosome 6 at 116,413,786 bp
  • T to C, chromosome 6 at 121,383,919 bp
  • T to A, chromosome 7 at 16,747,323 bp
  • A to T, chromosome 7 at 80,923,064 bp
  • A to G, chromosome 7 at 89,407,501 bp
  • T to C, chromosome 7 at 127,374,394 bp
  • T to A, chromosome 7 at 133,651,044 bp
  • G to T, chromosome 8 at 10,476,114 bp
  • G to T, chromosome 8 at 70,452,718 bp
  • T to G, chromosome 8 at 72,818,096 bp
  • T to C, chromosome 8 at 105,463,898 bp
  • T to C, chromosome 9 at 3,000,937 bp
  • T to A, chromosome 9 at 70,766,074 bp
  • C to T, chromosome 9 at 91,384,152 bp
  • A to T, chromosome 9 at 106,932,997 bp
  • T to G, chromosome 9 at 110,098,795 bp
  • A to G, chromosome 9 at 123,577,025 bp
  • T to A, chromosome 10 at 52,543,022 bp
  • A to G, chromosome 10 at 76,147,594 bp
  • T to C, chromosome 10 at 89,789,355 bp
  • T to C, chromosome 10 at 91,060,094 bp
  • T to C, chromosome 10 at 100,088,222 bp
  • A to T, chromosome 11 at 6,224,245 bp
  • A to T, chromosome 11 at 59,129,653 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • G to A, chromosome 11 at 79,527,451 bp
  • C to T, chromosome 11 at 105,857,553 bp
  • G to T, chromosome 11 at 118,264,905 bp
  • C to T, chromosome 12 at 8,010,086 bp
  • G to C, chromosome 12 at 81,377,830 bp
  • A to T, chromosome 12 at 113,574,462 bp
  • T to A, chromosome 12 at 116,427,786 bp
  • A to G, chromosome 13 at 9,170,737 bp
  • G to T, chromosome 13 at 11,662,130 bp
  • A to T, chromosome 13 at 27,579,819 bp
  • A to G, chromosome 13 at 33,880,097 bp
  • A to G, chromosome 13 at 41,102,959 bp
  • A to G, chromosome 13 at 93,627,323 bp
  • A to T, chromosome 13 at 100,179,440 bp
  • T to A, chromosome 14 at 26,770,477 bp
  • C to T, chromosome 14 at 47,399,665 bp
  • T to C, chromosome 14 at 52,463,854 bp
  • A to G, chromosome 15 at 65,883,830 bp
  • G to T, chromosome 15 at 71,799,337 bp
  • T to A, chromosome 15 at 101,627,768 bp
  • A to T, chromosome 16 at 21,956,165 bp
  • T to A, chromosome 17 at 34,243,516 bp
  • T to A, chromosome 17 at 78,866,345 bp
  • A to G, chromosome 18 at 47,291,172 bp
  • T to A, chromosome 19 at 6,320,618 bp
  • A to C, chromosome 19 at 40,554,817 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5135 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043261-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.