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Strain Name:
C57BL/6J-MtgxR5362Btlr/Mmmh
Stock Number:
043264-MU
Citation ID:
RRID:MMRRC_043264-MU
Other Names:
R5362 (G1)
Major Collection:

Strain Information

Nrp2
Name: neuropilin 2
Synonyms: NP2, Npn-2, Npn2, NP-2, 1110048P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Ano1
Name: anoctamin 1, calcium activated chloride channel
Synonyms: Tmem16a
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101772
Homologene: 75079
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Foxj3
Name: forkhead box J3
Synonyms: C330039G02Rik, Fhd6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230700
Homologene: 35243
Trappc9
Name: trafficking protein particle complex 9
Synonyms: 4632408O18Rik, 2900005P22Rik, Nibp, TRS130, 1810044A24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76510
VEGA: 15
Homologene: 81931
Top2a
Name: topoisomerase (DNA) II alpha
Synonyms: DNA Topoisomerase II alpha, Top-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21973
Homologene: 830
Prpf8
Name: pre-mRNA processing factor 8
Synonyms: DBF3/PRP8, Prp8, D11Bwg0410e, Sfprp8l
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192159
Homologene: 4706
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 62,769,062 bp
  • A to T, chromosome 1 at 92,854,893 bp
  • T to C, chromosome 1 at 192,847,106 bp
  • T to C, chromosome 2 at 41,375,902 bp
  • G to A, chromosome 2 at 66,699,998 bp
  • G to T, chromosome 2 at 122,061,000 bp
  • G to T, chromosome 2 at 148,783,378 bp
  • G to A, chromosome 3 at 32,992,916 bp
  • G to A, chromosome 3 at 84,515,693 bp
  • A to C, chromosome 4 at 56,778,969 bp
  • A to T, chromosome 4 at 76,128,813 bp
  • A to T, chromosome 4 at 92,146,800 bp
  • T to A, chromosome 4 at 119,620,143 bp
  • A to G, chromosome 4 at 155,438,166 bp
  • T to A, chromosome 5 at 87,694,649 bp
  • G to A, chromosome 5 at 90,265,545 bp
  • T to C, chromosome 5 at 144,023,856 bp
  • A to G, chromosome 6 at 49,376,115 bp
  • A to G, chromosome 6 at 122,932,237 bp
  • A to T, chromosome 6 at 133,294,482 bp
  • T to C, chromosome 6 at 143,083,243 bp
  • G to A, chromosome 7 at 19,523,268 bp
  • A to T, chromosome 7 at 20,101,461 bp
  • G to A, chromosome 7 at 25,092,895 bp
  • A to G, chromosome 7 at 103,346,247 bp
  • A to G, chromosome 7 at 104,916,627 bp
  • A to C, chromosome 7 at 144,648,600 bp
  • T to C, chromosome 8 at 11,245,760 bp
  • T to A, chromosome 8 at 105,381,398 bp
  • A to G, chromosome 9 at 21,587,359 bp
  • G to A, chromosome 9 at 110,489,944 bp
  • C to T, chromosome 10 at 79,912,458 bp
  • T to C, chromosome 10 at 129,874,553 bp
  • G to A, chromosome 11 at 34,402,788 bp
  • A to T, chromosome 11 at 49,216,612 bp
  • A to G, chromosome 11 at 73,111,849 bp
  • G to A, chromosome 11 at 75,506,410 bp
  • T to C, chromosome 11 at 95,120,500 bp
  • T to C, chromosome 11 at 99,018,912 bp
  • A to G, chromosome 11 at 102,461,135 bp
  • G to A, chromosome 11 at 106,208,213 bp
  • G to T, chromosome 11 at 115,117,114 bp
  • T to C, chromosome 11 at 116,447,150 bp
  • A to G, chromosome 14 at 49,960,529 bp
  • C to T, chromosome 15 at 73,058,217 bp
  • G to T, chromosome 17 at 19,379,339 bp
  • T to C, chromosome 17 at 79,861,461 bp
  • C to T, chromosome 18 at 5,057,345 bp
  • G to T, chromosome 18 at 67,421,259 bp
  • G to T, chromosome 19 at 29,811,728 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5362 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043264-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.