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Strain Name:
C57BL/6J-MtgxR5523Btlr/Mmmh
Stock Number:
043265-MU
Citation ID:
RRID:MMRRC_043265-MU
Other Names:
R5523 (G1)
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Acadm
Name: acyl-Coenzyme A dehydrogenase, medium chain
Synonyms: MCAD
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11364
HGNC: HGNC:89
Homologene: 3
Sptbn1
Name: spectrin beta, non-erythrocytic 1
Synonyms: beta fodrin, Spnb-2, spectrin G, brain spectrin, elf3, elf1, 9930031C03Rik, non-erythrocytic, Spnb2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20742
Homologene: 2354
Apoa5
Name: apolipoprotein A-V
Synonyms: RAP3, Apoav, 1300007O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66113
Homologene: 14197
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140919
Homologene: 121617
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,802,112 bp
  • T to C, chromosome 2 at 52,278,815 bp
  • A to T, chromosome 2 at 76,946,897 bp
  • T to C, chromosome 2 at 82,258,995 bp
  • C to A, chromosome 2 at 135,260,566 bp
  • T to C, chromosome 2 at 153,875,985 bp
  • T to A, chromosome 2 at 160,702,775 bp
  • C to T, chromosome 3 at 72,891,421 bp
  • A to G, chromosome 3 at 100,959,242 bp
  • G to A, chromosome 3 at 153,938,636 bp
  • A to T, chromosome 4 at 140,814,853 bp
  • T to C, chromosome 4 at 148,258,097 bp
  • A to G, chromosome 5 at 25,299,339 bp
  • C to A, chromosome 5 at 30,145,254 bp
  • A to G, chromosome 5 at 86,338,870 bp
  • A to C, chromosome 5 at 134,902,782 bp
  • A to T, chromosome 5 at 136,060,100 bp
  • A to G, chromosome 5 at 137,421,893 bp
  • G to C, chromosome 5 at 144,275,958 bp
  • T to C, chromosome 6 at 21,723,212 bp
  • A to T, chromosome 6 at 47,953,521 bp
  • G to A, chromosome 6 at 59,353,946 bp
  • C to A, chromosome 6 at 72,424,034 bp
  • T to C, chromosome 6 at 88,725,761 bp
  • T to A, chromosome 6 at 103,708,714 bp
  • A to G, chromosome 6 at 113,290,231 bp
  • A to C, chromosome 6 at 115,490,071 bp
  • T to C, chromosome 6 at 125,643,042 bp
  • A to T, chromosome 7 at 19,397,007 bp
  • A to T, chromosome 7 at 28,370,431 bp
  • A to T, chromosome 7 at 51,626,850 bp
  • G to A, chromosome 7 at 82,574,442 bp
  • T to A, chromosome 7 at 103,717,480 bp
  • G to A, chromosome 7 at 105,703,209 bp
  • A to G, chromosome 7 at 126,863,862 bp
  • G to T, chromosome 7 at 133,876,840 bp
  • G to T, chromosome 7 at 140,112,513 bp
  • A to G, chromosome 8 at 117,108,693 bp
  • G to A, chromosome 8 at 122,568,093 bp
  • T to C, chromosome 9 at 46,270,589 bp
  • A to T, chromosome 9 at 72,944,984 bp
  • A to G, chromosome 9 at 122,926,389 bp
  • A to T, chromosome 10 at 45,469,068 bp
  • T to A, chromosome 10 at 59,222,963 bp
  • A to G, chromosome 10 at 77,935,961 bp
  • G to T, chromosome 11 at 23,349,198 bp
  • A to G, chromosome 11 at 30,137,560 bp
  • C to A, chromosome 11 at 67,305,962 bp
  • T to C, chromosome 11 at 97,336,782 bp
  • A to G, chromosome 11 at 105,343,582 bp
  • T to C, chromosome 11 at 116,668,284 bp
  • T to A, chromosome 12 at 11,291,539 bp
  • A to G, chromosome 12 at 76,438,118 bp
  • T to A, chromosome 12 at 105,741,082 bp
  • T to C, chromosome 12 at 112,775,208 bp
  • T to C, chromosome 13 at 51,711,354 bp
  • T to C, chromosome 13 at 72,631,595 bp
  • A to G, chromosome 14 at 56,552,423 bp
  • G to A, chromosome 14 at 123,409,743 bp
  • A to G, chromosome 15 at 82,372,571 bp
  • T to A, chromosome 15 at 103,249,129 bp
  • C to T, chromosome 16 at 81,434,878 bp
  • G to A, chromosome 17 at 26,624,606 bp
  • C to A, chromosome 17 at 32,470,833 bp
  • T to A, chromosome 17 at 32,669,822 bp
  • T to C, chromosome 17 at 34,036,437 bp
  • C to T, chromosome 17 at 34,899,091 bp
  • A to T, chromosome 18 at 37,012,386 bp
  • T to C, chromosome 18 at 38,283,139 bp
  • A to G, chromosome 18 at 68,313,702 bp
  • T to G, chromosome 18 at 84,960,069 bp
  • A to G, chromosome 19 at 5,383,676 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5523 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043265-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.