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Strain Name:
C57BL/6J-MtgxR5621Btlr/Mmmh
Stock Number:
043279-MU
Citation ID:
RRID:MMRRC_043279-MU
Other Names:
R5621 (G1)
Major Collection:

Strain Information

Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Chrna6
Name: cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms: Acra6, alpha6 nAChR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11440
Homologene: 20888
Fgf8
Name: fibroblast growth factor 8
Synonyms: Fgf-8, Aigf
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14179
HGNC: HGNC:3686
Homologene: 7715
Stk17b
Name: serine/threonine kinase 17b (apoptosis-inducing)
Synonyms: Drak2, 3110009A03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98267
Homologene: 31231
Rrp12
Name: ribosomal RNA processing 12 homolog
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107094
VEGA: 19
Homologene: 22370
Kif5b
Name: kinesin family member 5B
Synonyms: Khc, kinesin heavy chain
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16573
HGNC: HGNC:6324
Homologene: 55829
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: 2610042O15Rik, Mms19, C86341, Mms19l
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72199
Homologene: 41480
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 39,359,713 bp
  • G to T, chromosome 1 at 53,771,784 bp
  • A to G, chromosome 1 at 59,191,890 bp
  • A to G, chromosome 1 at 66,638,043 bp
  • G to A, chromosome 1 at 74,380,906 bp
  • G to T, chromosome 1 at 77,515,049 bp
  • A to G, chromosome 2 at 6,440,432 bp
  • T to A, chromosome 2 at 10,102,805 bp
  • C to A, chromosome 2 at 32,371,741 bp
  • T to C, chromosome 2 at 88,973,466 bp
  • G to A, chromosome 2 at 89,282,353 bp
  • C to T, chromosome 2 at 110,292,523 bp
  • C to T, chromosome 2 at 112,900,984 bp
  • A to G, chromosome 2 at 139,678,721 bp
  • A to G, chromosome 2 at 178,381,918 bp
  • T to G, chromosome 3 at 50,438,875 bp
  • G to A, chromosome 3 at 92,428,357 bp
  • T to A, chromosome 4 at 32,716,371 bp
  • C to T, chromosome 4 at 34,811,703 bp
  • A to G, chromosome 4 at 132,553,252 bp
  • T to A, chromosome 5 at 64,112,038 bp
  • A to T, chromosome 5 at 72,108,728 bp
  • A to G, chromosome 7 at 28,466,836 bp
  • A to G, chromosome 7 at 48,643,368 bp
  • G to T, chromosome 7 at 65,701,618 bp
  • A to G, chromosome 8 at 18,632,170 bp
  • C to T, chromosome 8 at 27,407,040 bp
  • A to T, chromosome 8 at 63,144,006 bp
  • A to T, chromosome 9 at 7,120,909 bp
  • A to G, chromosome 9 at 65,278,791 bp
  • A to T, chromosome 10 at 18,216,268 bp
  • T to A, chromosome 11 at 20,648,061 bp
  • T to C, chromosome 11 at 59,450,393 bp
  • A to G, chromosome 11 at 86,242,947 bp
  • C to T, chromosome 11 at 116,536,366 bp
  • G to A, chromosome 13 at 22,278,389 bp
  • A to T, chromosome 14 at 16,387,280 bp
  • T to G, chromosome 15 at 48,313,978 bp
  • G to T, chromosome 15 at 57,259,151 bp
  • T to C, chromosome 15 at 58,558,326 bp
  • A to T, chromosome 15 at 91,844,420 bp
  • G to T, chromosome 16 at 4,039,118 bp
  • G to A, chromosome 16 at 14,244,855 bp
  • T to C, chromosome 16 at 75,766,763 bp
  • G to C, chromosome 17 at 56,620,263 bp
  • T to G, chromosome 17 at 84,695,993 bp
  • T to C, chromosome 18 at 6,226,883 bp
  • A to G, chromosome 18 at 37,682,169 bp
  • T to A, chromosome 19 at 4,619,167 bp
  • C to T, chromosome 19 at 8,071,011 bp
  • T to A, chromosome 19 at 36,618,751 bp
  • T to C, chromosome 19 at 41,880,417 bp
  • A to G, chromosome 19 at 41,966,313 bp
  • T to C, chromosome 19 at 45,742,382 bp
  • A to C, chromosome 19 at 55,079,260 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5621 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043279-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.