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Strain Name:
C57BL/6J-MtgxR5629Btlr
Stock Number:
043280-MU
Citation ID:
RRID:MMRRC_043280-MU
Other Names:
R5629 (G1)
Major Collection:

Strain Information

Hapln1
Name: hyaluronan and proteoglycan link protein 1
Synonyms: link protein, cartilage linking protein 1, Crtl1l, Crtl1, CLP, LP-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12950
VEGA: 13
HGNC: HGNC:2380
Homologene: 1420
Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Zic4
Name: zinc finger protein of the cerebellum 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22774
Homologene: 32075
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67414
Homologene: 11481
Ctnna1
Name: catenin alpha 1
Synonyms: alpha(E)-catenin, alpha E catenin, Catna1, 2010010M04Rik, catenin (cadherin associated protein), alpha 1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12385
VEGA: 18
HGNC: HGNC:2509
Homologene: 1433
Etv4
Name: ets variant 4
Synonyms: Pea-3, Pea3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18612
HGNC: HGNC:3493
Homologene: 1504
Nsfl1c
Name: NSFL1 (p97) cofactor (p47)
Synonyms: p47
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 386649
Homologene: 41114
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 87,981,009 bp
  • C to T, chromosome 1 at 130,582,879 bp
  • T to C, chromosome 1 at 162,648,587 bp
  • A to T, chromosome 2 at 9,918,178 bp
  • T to C, chromosome 2 at 70,965,569 bp
  • T to A, chromosome 2 at 128,596,785 bp
  • T to C, chromosome 2 at 151,504,165 bp
  • T to A, chromosome 2 at 164,290,120 bp
  • A to T, chromosome 3 at 32,561,510 bp
  • G to A, chromosome 3 at 64,105,117 bp
  • A to T, chromosome 3 at 100,080,119 bp
  • G to A, chromosome 4 at 113,012,979 bp
  • T to A, chromosome 4 at 119,256,919 bp
  • A to G, chromosome 5 at 21,675,903 bp
  • G to A, chromosome 5 at 35,857,163 bp
  • G to A, chromosome 5 at 37,457,232 bp
  • G to A, chromosome 5 at 120,738,451 bp
  • A to G, chromosome 5 at 122,460,096 bp
  • T to A, chromosome 5 at 135,742,050 bp
  • A to G, chromosome 6 at 18,405,218 bp
  • A to T, chromosome 6 at 48,570,260 bp
  • A to T, chromosome 6 at 92,798,133 bp
  • A to G, chromosome 7 at 14,562,086 bp
  • A to C, chromosome 7 at 89,510,192 bp
  • A to G, chromosome 7 at 102,107,079 bp
  • A to T, chromosome 7 at 102,774,640 bp
  • T to C, chromosome 7 at 104,487,929 bp
  • T to A, chromosome 7 at 122,170,166 bp
  • T to A, chromosome 7 at 128,444,697 bp
  • T to C, chromosome 7 at 141,861,299 bp
  • C to A, chromosome 8 at 125,898,041 bp
  • T to A, chromosome 9 at 25,288,293 bp
  • G to A, chromosome 9 at 37,408,362 bp
  • G to A, chromosome 9 at 37,419,211 bp
  • A to T, chromosome 9 at 44,356,376 bp
  • A to T, chromosome 9 at 75,203,845 bp
  • G to A, chromosome 9 at 91,378,752 bp
  • A to G, chromosome 9 at 108,849,067 bp
  • A to G, chromosome 9 at 123,566,399 bp
  • G to A, chromosome 10 at 112,078,403 bp
  • A to G, chromosome 10 at 115,347,260 bp
  • C to T, chromosome 11 at 60,479,752 bp
  • T to A, chromosome 11 at 101,771,925 bp
  • A to C, chromosome 11 at 102,404,893 bp
  • G to T, chromosome 11 at 120,377,011 bp
  • T to C, chromosome 12 at 8,007,847 bp
  • G to A, chromosome 12 at 81,199,631 bp
  • T to C, chromosome 12 at 103,425,547 bp
  • A to T, chromosome 12 at 113,625,622 bp
  • A to T, chromosome 13 at 23,481,960 bp
  • A to T, chromosome 13 at 76,093,908 bp
  • A to G, chromosome 13 at 89,601,515 bp
  • T to A, chromosome 13 at 95,632,174 bp
  • T to C, chromosome 14 at 50,927,916 bp
  • C to T, chromosome 14 at 70,422,967 bp
  • A to G, chromosome 15 at 58,054,811 bp
  • A to T, chromosome 15 at 77,718,076 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • T to A, chromosome 16 at 32,144,405 bp
  • C to A, chromosome 16 at 32,332,299 bp
  • T to C, chromosome 16 at 34,039,934 bp
  • T to C, chromosome 16 at 72,983,710 bp
  • T to A, chromosome 16 at 88,729,159 bp
  • T to C, chromosome 17 at 24,722,682 bp
  • A to T, chromosome 17 at 90,590,032 bp
  • T to A, chromosome 18 at 24,664,085 bp
  • T to A, chromosome 18 at 35,249,749 bp
  • T to C, chromosome 18 at 35,626,492 bp
  • T to A, chromosome 18 at 36,788,666 bp
  • T to C, chromosome 18 at 37,643,502 bp
  • C to T, chromosome 19 at 5,336,137 bp
  • T to C, chromosome 19 at 16,436,733 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5629 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043280-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.