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Strain Name:
C57BL/6J-MtgxR5633Btlr/Mmmh
Stock Number:
043284-MU
Citation ID:
RRID:MMRRC_043284-MU
Other Names:
R5633 (G1)
Major Collection:

Strain Information

Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Elp2
Name: elongator acetyltransferase complex subunit 2
Synonyms: Stat3-interacting protein, StIP1, Statip1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 58523
VEGA: 18
Homologene: 6019
Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Rnf145
Name: ring finger protein 145
Synonyms: 3732413I11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74315
Homologene: 14427
P4htm
Name: prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Synonyms: 4933406E20Rik, P4h-tm
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74443
Homologene: 41765
Dmtn
Name: dematin actin binding protein
Synonyms: dematin, Epb4.9
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13829
VEGA: 14
HGNC: HGNC:3382
Homologene: 1496
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 24,748,862 bp
  • A to G, chromosome 1 at 65,165,136 bp
  • G to A, chromosome 1 at 69,539,097 bp
  • A to G, chromosome 1 at 131,846,587 bp
  • A to T, chromosome 1 at 180,327,225 bp
  • T to A, chromosome 1 at 180,669,198 bp
  • A to G, chromosome 2 at 25,363,349 bp
  • A to G, chromosome 2 at 34,856,488 bp
  • C to G, chromosome 2 at 121,363,629 bp
  • T to A, chromosome 2 at 157,283,596 bp
  • T to C, chromosome 2 at 181,029,460 bp
  • T to A, chromosome 3 at 69,008,110 bp
  • T to A, chromosome 3 at 103,178,424 bp
  • C to A, chromosome 3 at 106,388,752 bp
  • T to A, chromosome 4 at 45,881,455 bp
  • T to A, chromosome 4 at 144,618,028 bp
  • T to C, chromosome 5 at 24,403,109 bp
  • T to C, chromosome 5 at 90,604,118 bp
  • T to A, chromosome 5 at 104,498,506 bp
  • T to C, chromosome 5 at 111,420,326 bp
  • C to T, chromosome 5 at 123,819,057 bp
  • T to C, chromosome 5 at 137,529,192 bp
  • A to T, chromosome 6 at 8,667,257 bp
  • A to T, chromosome 7 at 20,101,945 bp
  • T to C, chromosome 7 at 100,507,419 bp
  • C to T, chromosome 7 at 107,425,296 bp
  • C to A, chromosome 7 at 120,873,290 bp
  • T to C, chromosome 7 at 143,847,423 bp
  • T to A, chromosome 9 at 59,868,184 bp
  • C to T, chromosome 9 at 75,344,514 bp
  • A to C, chromosome 9 at 108,579,723 bp
  • T to C, chromosome 10 at 77,938,353 bp
  • A to T, chromosome 10 at 129,186,849 bp
  • T to C, chromosome 11 at 44,560,088 bp
  • C to T, chromosome 11 at 46,574,606 bp
  • A to T, chromosome 11 at 54,337,189 bp
  • G to T, chromosome 11 at 61,369,261 bp
  • A to G, chromosome 11 at 86,278,931 bp
  • T to C, chromosome 11 at 98,554,758 bp
  • C to A, chromosome 11 at 99,864,586 bp
  • A to G, chromosome 12 at 84,058,950 bp
  • C to T, chromosome 13 at 116,876,580 bp
  • T to G, chromosome 14 at 31,735,065 bp
  • T to C, chromosome 14 at 70,604,979 bp
  • A to T, chromosome 15 at 36,162,095 bp
  • A to G, chromosome 15 at 64,699,285 bp
  • A to T, chromosome 15 at 79,299,142 bp
  • A to T, chromosome 16 at 96,315,509 bp
  • T to A, chromosome 18 at 24,615,210 bp
  • T to A, chromosome 18 at 49,877,697 bp
  • T to C, chromosome 18 at 84,561,044 bp
  • A to G, chromosome 19 at 9,992,103 bp
  • T to C, chromosome 19 at 36,086,990 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5633 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043284-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.