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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR5644Btlr/Mmmh
Stock Number:
043292-MU
Citation ID:
RRID:MMRRC_043292-MU
Other Names:
R5644 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78514
Homologene: 12695

Additional Resources
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IMSR
Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Rai14
Name: retinoic acid induced 14
Synonyms: 1700020L11Rik, Ankycorbin, Norpeg, 1700008J19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 33,746,953 bp
  • C to T, chromosome 1 at 39,370,027 bp
  • A to T, chromosome 1 at 53,540,979 bp
  • A to T, chromosome 1 at 71,627,250 bp
  • T to A, chromosome 1 at 88,359,739 bp
  • A to C, chromosome 1 at 100,383,601 bp
  • A to G, chromosome 1 at 119,932,802 bp
  • A to G, chromosome 1 at 121,483,336 bp
  • A to T, chromosome 1 at 169,955,948 bp
  • A to T, chromosome 2 at 10,568,373 bp
  • A to T, chromosome 2 at 24,014,239 bp
  • A to G, chromosome 2 at 69,670,471 bp
  • T to A, chromosome 2 at 76,938,523 bp
  • T to C, chromosome 2 at 86,085,159 bp
  • T to A, chromosome 2 at 88,559,505 bp
  • A to T, chromosome 2 at 112,071,668 bp
  • A to T, chromosome 2 at 149,899,508 bp
  • A to T, chromosome 2 at 177,507,261 bp
  • A to T, chromosome 3 at 59,218,095 bp
  • C to T, chromosome 3 at 141,678,125 bp
  • T to C, chromosome 4 at 40,984,973 bp
  • T to A, chromosome 4 at 43,483,562 bp
  • T to A, chromosome 4 at 63,165,893 bp
  • C to T, chromosome 4 at 116,098,947 bp
  • T to A, chromosome 4 at 118,965,236 bp
  • T to C, chromosome 4 at 123,049,172 bp
  • T to C, chromosome 4 at 135,863,820 bp
  • C to T, chromosome 4 at 143,948,804 bp
  • T to C, chromosome 4 at 149,238,482 bp
  • T to C, chromosome 4 at 152,104,340 bp
  • A to T, chromosome 5 at 65,416,861 bp
  • C to T, chromosome 5 at 151,649,979 bp
  • A to G, chromosome 6 at 3,708,538 bp
  • G to A, chromosome 6 at 42,642,773 bp
  • T to C, chromosome 6 at 89,870,372 bp
  • T to A, chromosome 6 at 129,379,960 bp
  • A to T, chromosome 6 at 129,656,457 bp
  • A to C, chromosome 6 at 142,237,594 bp
  • C to T, chromosome 6 at 149,520,403 bp
  • A to T, chromosome 7 at 26,558,568 bp
  • T to C, chromosome 7 at 27,703,769 bp
  • T to C, chromosome 7 at 44,507,053 bp
  • G to A, chromosome 7 at 48,168,207 bp
  • T to C, chromosome 7 at 63,891,560 bp
  • A to G, chromosome 7 at 64,715,511 bp
  • T to C, chromosome 7 at 83,989,184 bp
  • C to A, chromosome 7 at 84,916,119 bp
  • A to T, chromosome 7 at 108,094,804 bp
  • A to G, chromosome 7 at 108,124,651 bp
  • G to T, chromosome 8 at 41,360,295 bp
  • A to G, chromosome 8 at 69,139,077 bp
  • C to T, chromosome 8 at 77,411,055 bp
  • A to G, chromosome 8 at 84,462,777 bp
  • T to A, chromosome 8 at 85,094,210 bp
  • G to T, chromosome 9 at 3,385,384 bp
  • G to T, chromosome 9 at 18,334,931 bp
  • T to C, chromosome 9 at 31,106,510 bp
  • T to C, chromosome 9 at 37,649,413 bp
  • A to G, chromosome 9 at 48,392,750 bp
  • G to A, chromosome 9 at 65,045,735 bp
  • G to T, chromosome 9 at 86,790,153 bp
  • A to T, chromosome 10 at 14,432,934 bp
  • T to C, chromosome 10 at 62,771,978 bp
  • T to A, chromosome 10 at 94,134,050 bp
  • A to T, chromosome 10 at 129,527,103 bp
  • T to A, chromosome 10 at 129,857,427 bp
  • A to T, chromosome 11 at 82,722,455 bp
  • T to C, chromosome 11 at 99,559,312 bp
  • T to A, chromosome 11 at 116,973,400 bp
  • T to A, chromosome 11 at 119,280,535 bp
  • T to A, chromosome 12 at 11,289,994 bp
  • T to A, chromosome 12 at 30,884,890 bp
  • A to T, chromosome 12 at 91,348,851 bp
  • C to T, chromosome 12 at 109,591,579 bp
  • A to T, chromosome 12 at 116,000,060 bp
  • C to A, chromosome 13 at 11,595,582 bp
  • A to T, chromosome 13 at 13,637,496 bp
  • A to G, chromosome 13 at 21,423,152 bp
  • T to A, chromosome 13 at 47,028,959 bp
  • T to C, chromosome 13 at 66,972,540 bp
  • T to C, chromosome 13 at 113,098,260 bp
  • C to A, chromosome 14 at 30,607,413 bp
  • A to G, chromosome 14 at 101,929,336 bp
  • T to A, chromosome 14 at 103,287,334 bp
  • G to A, chromosome 14 at 122,887,069 bp
  • C to T, chromosome 15 at 8,358,907 bp
  • A to T, chromosome 15 at 10,461,915 bp
  • T to A, chromosome 15 at 10,593,051 bp
  • T to A, chromosome 16 at 20,526,276 bp
  • T to C, chromosome 16 at 56,184,149 bp
  • T to C, chromosome 17 at 34,742,417 bp
  • T to C, chromosome 17 at 36,866,821 bp
  • T to A, chromosome 17 at 37,624,432 bp
  • T to A, chromosome 17 at 57,524,977 bp
  • T to C, chromosome 17 at 80,238,873 bp
  • A to T, chromosome 18 at 62,178,682 bp
  • A to G, chromosome 19 at 5,961,170 bp
  • T to C, chromosome 19 at 8,404,616 bp
  • T to A, chromosome 19 at 8,737,876 bp
  • A to G, chromosome 19 at 9,010,657 bp
  • A to T, chromosome 19 at 12,680,972 bp
  • A to C, chromosome 19 at 39,615,694 bp
  • A to G, chromosome 19 at 45,040,180 bp
  • A to G, chromosome 19 at 47,179,477 bp
  • T to A, chromosome 19 at 47,795,675 bp
  • A to T, chromosome 19 at 55,088,899 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5644 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
043292-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.