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Strain Name:
C57BL/6J-MtgxR5645Btlr/Mmmh
Stock Number:
043293-MU
Citation ID:
RRID:MMRRC_043293-MU
Other Names:
R5645 (G1)
Major Collection:

Strain Information

Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Ano6
Name: anoctamin 6
Synonyms: 2900059G15Rik, F730003B03Rik, Tmem16f
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105722
VEGA: 15
Homologene: 27888
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320365
Homologene: 113770
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 6030449K19Rik, 2900036C23Rik, 2410152H17Rik, 2810413I22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66315
Homologene: 10778
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57752
Homologene: 5087
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,588,381 bp
  • T to C, chromosome 1 at 66,801,344 bp
  • T to C, chromosome 1 at 93,152,946 bp
  • G to T, chromosome 1 at 195,154,273 bp
  • T to G, chromosome 2 at 26,439,895 bp
  • C to A, chromosome 2 at 31,420,812 bp
  • A to T, chromosome 2 at 89,339,705 bp
  • T to C, chromosome 2 at 147,184,399 bp
  • C to T, chromosome 2 at 163,407,045 bp
  • T to A, chromosome 3 at 14,645,120 bp
  • T to C, chromosome 3 at 88,117,699 bp
  • C to T, chromosome 3 at 146,650,509 bp
  • A to T, chromosome 3 at 152,656,033 bp
  • G to T, chromosome 4 at 43,425,758 bp
  • T to C, chromosome 4 at 56,776,920 bp
  • G to A, chromosome 4 at 108,557,373 bp
  • A to T, chromosome 4 at 111,657,330 bp
  • A to G, chromosome 4 at 112,025,502 bp
  • A to T, chromosome 4 at 129,616,527 bp
  • G to T, chromosome 4 at 136,612,917 bp
  • A to G, chromosome 5 at 4,050,590 bp
  • T to C, chromosome 5 at 150,380,867 bp
  • T to C, chromosome 6 at 141,834,659 bp
  • A to G, chromosome 7 at 4,145,274 bp
  • G to A, chromosome 7 at 63,891,600 bp
  • A to G, chromosome 7 at 64,695,806 bp
  • A to G, chromosome 7 at 119,640,697 bp
  • A to T, chromosome 7 at 130,624,051 bp
  • G to A, chromosome 7 at 140,248,940 bp
  • T to G, chromosome 8 at 60,896,800 bp
  • T to C, chromosome 8 at 105,357,013 bp
  • A to G, chromosome 8 at 123,247,748 bp
  • A to G, chromosome 9 at 4,331,426 bp
  • A to T, chromosome 9 at 15,332,794 bp
  • C to A, chromosome 9 at 15,335,108 bp
  • G to A, chromosome 9 at 20,922,147 bp
  • G to T, chromosome 9 at 26,824,826 bp
  • A to G, chromosome 9 at 44,682,678 bp
  • T to C, chromosome 9 at 53,622,943 bp
  • T to A, chromosome 9 at 75,025,679 bp
  • T to C, chromosome 9 at 99,014,191 bp
  • A to G, chromosome 9 at 108,397,204 bp
  • T to C, chromosome 10 at 41,552,356 bp
  • T to C, chromosome 10 at 81,278,620 bp
  • T to A, chromosome 11 at 23,015,725 bp
  • T to C, chromosome 11 at 23,375,024 bp
  • A to G, chromosome 11 at 68,991,132 bp
  • A to T, chromosome 11 at 72,351,837 bp
  • A to G, chromosome 11 at 73,129,248 bp
  • T to C, chromosome 11 at 75,756,924 bp
  • T to C, chromosome 11 at 88,845,627 bp
  • T to C, chromosome 11 at 110,250,408 bp
  • T to A, chromosome 11 at 115,446,785 bp
  • T to A, chromosome 12 at 21,265,982 bp
  • C to A, chromosome 13 at 68,729,202 bp
  • A to G, chromosome 13 at 74,323,839 bp
  • T to C, chromosome 14 at 50,643,067 bp
  • A to T, chromosome 14 at 103,188,608 bp
  • C to A, chromosome 14 at 103,188,615 bp
  • A to G, chromosome 15 at 11,277,420 bp
  • A to G, chromosome 15 at 44,532,992 bp
  • A to G, chromosome 15 at 95,920,351 bp
  • A to G, chromosome 15 at 98,844,397 bp
  • T to A, chromosome 16 at 9,992,226 bp
  • A to G, chromosome 16 at 33,706,963 bp
  • A to G, chromosome 16 at 44,499,661 bp
  • T to A, chromosome 16 at 56,173,208 bp
  • A to G, chromosome 16 at 91,404,227 bp
  • G to T, chromosome 17 at 25,737,842 bp
  • G to T, chromosome 17 at 46,910,667 bp
  • A to G, chromosome 17 at 67,802,948 bp
  • A to G, chromosome 18 at 52,894,956 bp
  • A to G, chromosome 19 at 18,853,604 bp
  • G to A, chromosome 19 at 39,994,152 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5645 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043293-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.