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Strain Name:
C57BL/6J-MtgxR5653Btlr/Mmmh
Stock Number:
043299-MU
Citation ID:
RRID:MMRRC_043299-MU
Other Names:
R5653 (G1)
Major Collection:

Strain Information

Baz1b
Name: bromodomain adjacent to zinc finger domain, 1B
Synonyms: Wbscr9, WSTF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22385
HGNC: HGNC:961
Homologene: 22651
Kat6b
Name: K(lysine) acetyltransferase 6B
Synonyms: qkf, querkopf, Morf, B130044K16Rik, monocytic leukemia, Myst4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 54169
Homologene: 136480
Dnm1l
Name: dynamin 1-like
Synonyms: 6330417M19Rik, Drp1, python, Dnmlp1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74006
VEGA: 16
HGNC: HGNC:2973
Homologene: 6384
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: SF21, 2610528O22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Gtf2ird1
Name: general transcription factor II I repeat domain-containing 1
Synonyms: WBSCR11, Cream1, GTF3, MusTRD1, binding factor for early enhancer, BEN, ESTM9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57080
HGNC: HGNC:4661
Homologene: 4158
Bard1
Name: BRCA1 associated RING domain 1
Synonyms: ENSMUSG00000060893, ENSMUSG00000073653
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12021
HGNC: HGNC:952
Homologene: 400
Cacnb1
Name: calcium channel, voltage-dependent, beta 1 subunit
Synonyms: Cchlb1, Cchb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12295
HGNC: HGNC:1401
Homologene: 20186
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 71,031,429 bp
  • A to G, chromosome 1 at 156,656,510 bp
  • T to A, chromosome 2 at 80,349,368 bp
  • T to A, chromosome 2 at 98,664,051 bp
  • A to G, chromosome 3 at 84,497,795 bp
  • A to T, chromosome 3 at 85,722,501 bp
  • T to A, chromosome 4 at 106,458,916 bp
  • C to A, chromosome 4 at 120,702,411 bp
  • T to A, chromosome 5 at 25,248,858 bp
  • T to C, chromosome 5 at 35,883,412 bp
  • A to G, chromosome 5 at 43,722,462 bp
  • A to T, chromosome 5 at 72,264,067 bp
  • A to G, chromosome 5 at 123,499,580 bp
  • A to T, chromosome 5 at 134,410,967 bp
  • A to T, chromosome 5 at 135,209,097 bp
  • G to A, chromosome 6 at 17,654,113 bp
  • A to G, chromosome 6 at 32,517,616 bp
  • T to C, chromosome 6 at 53,817,985 bp
  • T to C, chromosome 6 at 89,654,085 bp
  • T to C, chromosome 7 at 9,097,765 bp
  • T to A, chromosome 7 at 25,398,408 bp
  • C to T, chromosome 7 at 27,517,604 bp
  • G to T, chromosome 7 at 92,858,729 bp
  • A to T, chromosome 7 at 107,990,385 bp
  • T to C, chromosome 7 at 135,653,943 bp
  • T to G, chromosome 8 at 94,636,611 bp
  • A to G, chromosome 9 at 69,428,380 bp
  • A to T, chromosome 9 at 110,524,931 bp
  • A to G, chromosome 9 at 121,798,787 bp
  • A to G, chromosome 10 at 123,026,094 bp
  • C to T, chromosome 11 at 16,911,617 bp
  • T to C, chromosome 11 at 55,310,316 bp
  • G to A, chromosome 11 at 58,720,251 bp
  • T to C, chromosome 11 at 65,849,980 bp
  • C to T, chromosome 11 at 69,853,585 bp
  • A to T, chromosome 11 at 98,009,279 bp
  • C to T, chromosome 11 at 115,984,157 bp
  • T to A, chromosome 13 at 22,827,208 bp
  • A to T, chromosome 13 at 89,131,812 bp
  • A to G, chromosome 14 at 21,669,372 bp
  • T to C, chromosome 14 at 54,427,229 bp
  • T to A, chromosome 14 at 70,490,094 bp
  • A to T, chromosome 15 at 54,259,866 bp
  • C to T, chromosome 15 at 89,108,612 bp
  • C to T, chromosome 15 at 102,487,089 bp
  • A to G, chromosome 16 at 16,319,489 bp
  • T to A, chromosome 16 at 37,040,534 bp
  • A to G, chromosome 16 at 58,872,484 bp
  • C to T, chromosome 17 at 11,237,649 bp
  • T to C, chromosome 17 at 34,977,420 bp
  • C to T, chromosome 17 at 35,686,508 bp
  • T to A, chromosome 17 at 38,131,184 bp
  • A to G, chromosome 18 at 64,545,197 bp
  • C to T, chromosome 19 at 43,691,157 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5653 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043299-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.