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Strain Name:
C57BL/6J-MtgxR5654Btlr/Mmmh
Stock Number:
043300-MU
Citation ID:
RRID:MMRRC_043300-MU
Other Names:
R5654 (G1)
Major Collection:

Strain Information

Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Cs
Name: citrate synthase
Synonyms: Cis, 2610511A05Rik, 9030605P22Rik, ahl4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12974
VEGA: 10
HGNC: HGNC:2422
Homologene: 56073
Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: Pck-1, PEPCK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Cpne1
Name: copine I
Synonyms: 1810028N16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 266692
HGNC: HGNC:2314
Homologene: 36501
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 44,211,095 bp
  • T to C, chromosome 1 at 75,174,835 bp
  • C to T, chromosome 1 at 91,445,501 bp
  • C to T, chromosome 1 at 97,864,398 bp
  • C to T, chromosome 1 at 133,131,644 bp
  • A to T, chromosome 1 at 135,385,472 bp
  • A to G, chromosome 2 at 85,976,156 bp
  • T to A, chromosome 2 at 111,754,981 bp
  • G to A, chromosome 2 at 121,316,676 bp
  • G to A, chromosome 2 at 126,375,805 bp
  • T to C, chromosome 2 at 140,164,228 bp
  • T to C, chromosome 2 at 155,574,655 bp
  • A to G, chromosome 2 at 156,077,641 bp
  • T to A, chromosome 2 at 173,158,560 bp
  • A to C, chromosome 3 at 87,257,544 bp
  • T to C, chromosome 4 at 3,172,261 bp
  • C to T, chromosome 4 at 24,800,805 bp
  • A to G, chromosome 5 at 33,146,611 bp
  • T to A, chromosome 5 at 107,903,648 bp
  • T to C, chromosome 5 at 124,490,472 bp
  • A to G, chromosome 5 at 141,936,098 bp
  • A to G, chromosome 5 at 142,885,803 bp
  • T to C, chromosome 5 at 149,209,711 bp
  • G to T, chromosome 6 at 22,986,134 bp
  • A to G, chromosome 6 at 30,117,062 bp
  • T to G, chromosome 6 at 125,153,067 bp
  • G to A, chromosome 6 at 142,625,645 bp
  • T to C, chromosome 7 at 4,808,432 bp
  • G to A, chromosome 7 at 13,261,168 bp
  • T to A, chromosome 7 at 43,778,386 bp
  • A to T, chromosome 7 at 44,191,465 bp
  • A to G, chromosome 7 at 47,427,405 bp
  • A to C, chromosome 7 at 103,777,975 bp
  • G to A, chromosome 7 at 107,074,187 bp
  • G to A, chromosome 8 at 13,171,388 bp
  • C to T, chromosome 8 at 24,587,803 bp
  • G to T, chromosome 9 at 87,047,480 bp
  • A to G, chromosome 9 at 92,593,823 bp
  • C to T, chromosome 10 at 18,143,062 bp
  • T to A, chromosome 10 at 34,102,707 bp
  • T to A, chromosome 10 at 67,230,006 bp
  • T to C, chromosome 10 at 127,517,799 bp
  • G to A, chromosome 10 at 128,351,217 bp
  • T to C, chromosome 11 at 60,906,066 bp
  • C to T, chromosome 11 at 73,712,138 bp
  • T to A, chromosome 11 at 94,090,712 bp
  • T to C, chromosome 11 at 115,800,079 bp
  • C to T, chromosome 11 at 115,984,157 bp
  • T to A, chromosome 12 at 13,583,475 bp
  • A to G, chromosome 12 at 44,564,058 bp
  • C to A, chromosome 13 at 12,478,280 bp
  • T to A, chromosome 13 at 46,674,338 bp
  • A to G, chromosome 13 at 54,736,536 bp
  • C to A, chromosome 13 at 98,692,066 bp
  • T to C, chromosome 14 at 33,107,618 bp
  • A to G, chromosome 14 at 55,659,008 bp
  • G to A, chromosome 14 at 75,724,799 bp
  • A to T, chromosome 15 at 31,485,936 bp
  • T to A, chromosome 15 at 89,521,326 bp
  • C to T, chromosome 17 at 11,237,649 bp
  • A to T, chromosome 17 at 24,881,465 bp
  • A to T, chromosome 17 at 26,016,064 bp
  • C to T, chromosome 17 at 35,686,508 bp
  • A to G, chromosome 17 at 83,215,908 bp
  • A to G, chromosome 18 at 31,583,240 bp
  • G to C, chromosome 18 at 63,145,091 bp
  • A to T, chromosome 18 at 73,970,294 bp
  • G to A, chromosome 18 at 89,048,432 bp
  • A to T, chromosome 19 at 36,983,615 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5654 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043300-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.