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Strain Name:
C57BL/6J-MtgxR5691Btlr/Mmmh
Stock Number:
043324-MU
Citation ID:
RRID:MMRRC_043324-MU
Other Names:
R5691 (G1)
Major Collection:

Strain Information

Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Kdm4c
Name: lysine (K)-specific demethylase 4C
Synonyms: 2410141F18Rik, Jmjd2c
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76804
Homologene: 41004
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Setd3
Name: SET domain containing 3
Synonyms: 2610305M23Rik, D12Ertd771e, 2610102I01Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 52690
Homologene: 41748
Dlgap4
Name: DLG associated protein 4
Synonyms: WBP16, SAP90/PSD-95-associated protein 4, PSD-95/SAP90 binding protein 4, DAP4, Sapap4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228836
Homologene: 8935
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, H3a, sirm, Sh3bp3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20402
Homologene: 40787
Trps1
Name: transcriptional repressor GATA binding 1
Synonyms: D15Ertd586e, trichorhinophalangeal syndrome I (human)
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 83925
Homologene: 8556
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,180,550 bp
  • T to G, chromosome 1 at 17,004,363 bp
  • A to T, chromosome 1 at 134,540,991 bp
  • A to T, chromosome 1 at 160,735,684 bp
  • A to G, chromosome 1 at 173,732,426 bp
  • T to A, chromosome 2 at 5,823,175 bp
  • T to C, chromosome 2 at 25,573,695 bp
  • A to T, chromosome 2 at 58,047,764 bp
  • T to C, chromosome 2 at 69,502,553 bp
  • A to G, chromosome 2 at 72,971,459 bp
  • A to T, chromosome 2 at 86,959,272 bp
  • G to A, chromosome 2 at 120,524,471 bp
  • A to G, chromosome 2 at 156,704,470 bp
  • C to T, chromosome 3 at 58,586,026 bp
  • C to T, chromosome 3 at 145,933,149 bp
  • T to A, chromosome 4 at 14,769,648 bp
  • G to A, chromosome 4 at 41,035,510 bp
  • A to T, chromosome 4 at 74,334,728 bp
  • C to A, chromosome 4 at 94,923,728 bp
  • C to A, chromosome 4 at 106,702,618 bp
  • A to C, chromosome 4 at 141,628,289 bp
  • A to T, chromosome 4 at 143,812,009 bp
  • G to A, chromosome 5 at 110,267,093 bp
  • C to A, chromosome 5 at 114,899,767 bp
  • C to T, chromosome 5 at 120,602,506 bp
  • A to G, chromosome 5 at 121,348,815 bp
  • A to G, chromosome 5 at 138,172,391 bp
  • C to T, chromosome 6 at 124,855,153 bp
  • A to T, chromosome 6 at 141,994,855 bp
  • G to A, chromosome 7 at 28,737,472 bp
  • C to A, chromosome 7 at 64,354,370 bp
  • A to G, chromosome 7 at 85,858,091 bp
  • T to C, chromosome 7 at 118,541,893 bp
  • T to C, chromosome 7 at 139,606,700 bp
  • C to T, chromosome 7 at 143,812,579 bp
  • T to A, chromosome 8 at 19,242,133 bp
  • T to A, chromosome 8 at 81,890,694 bp
  • C to A, chromosome 8 at 106,425,078 bp
  • T to A, chromosome 10 at 130,354,769 bp
  • A to G, chromosome 11 at 115,348,819 bp
  • T to C, chromosome 12 at 44,564,256 bp
  • T to C, chromosome 12 at 46,816,955 bp
  • G to A, chromosome 12 at 52,105,276 bp
  • TCCAGGTAGGGCACACC to TCC, chromosome 12 at 76,027,856 bp
  • C to T, chromosome 12 at 83,951,692 bp
  • C to A, chromosome 12 at 105,202,547 bp
  • A to T, chromosome 12 at 108,160,285 bp
  • A to T, chromosome 12 at 118,927,235 bp
  • A to T, chromosome 13 at 8,942,657 bp
  • A to G, chromosome 14 at 56,812,373 bp
  • G to T, chromosome 14 at 88,123,047 bp
  • T to A, chromosome 15 at 50,827,304 bp
  • A to G, chromosome 16 at 35,863,539 bp
  • A to G, chromosome 17 at 23,868,785 bp
  • T to C, chromosome 18 at 71,575,083 bp
  • T to C, chromosome 19 at 7,926,670 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5691 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043324-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.