Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5695Btlr
Stock Number:
043326-MU
Citation ID:
RRID:MMRRC_043326-MU
Other Names:
R5695 (G1)
Major Collection:

Strain Information

Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Cdca2
Name: cell division cycle associated 2
Synonyms: 2610311M19Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 108912
Homologene: 18444
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Epc2
Name: enhancer of polycomb homolog 2
Synonyms: D2Ertd694e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227867
Homologene: 32274
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 133,680,578 bp
  • G to A, chromosome 1 at 139,479,669 bp
  • A to T, chromosome 1 at 192,717,019 bp
  • G to A, chromosome 2 at 4,763,241 bp
  • A to G, chromosome 2 at 25,084,334 bp
  • C to A, chromosome 2 at 32,034,373 bp
  • T to A, chromosome 2 at 49,547,607 bp
  • T to A, chromosome 2 at 103,266,779 bp
  • T to C, chromosome 2 at 146,333,477 bp
  • G to A, chromosome 2 at 153,942,923 bp
  • G to A, chromosome 2 at 166,132,758 bp
  • TGAGCA to TGAGCAGAGCA, chromosome 3 at 88,896,216 bp
  • A to G, chromosome 3 at 93,149,023 bp
  • G to A, chromosome 3 at 94,773,635 bp
  • C to T, chromosome 3 at 129,309,099 bp
  • G to T, chromosome 3 at 154,733,573 bp
  • A to T, chromosome 4 at 45,826,374 bp
  • T to A, chromosome 4 at 138,163,433 bp
  • A to T, chromosome 5 at 28,339,012 bp
  • C to T, chromosome 5 at 52,862,239 bp
  • A to G, chromosome 5 at 96,781,344 bp
  • T to C, chromosome 5 at 120,915,011 bp
  • C to T, chromosome 5 at 137,734,984 bp
  • A to T, chromosome 6 at 38,818,875 bp
  • A to T, chromosome 6 at 58,008,753 bp
  • T to A, chromosome 7 at 9,025,151 bp
  • A to T, chromosome 7 at 100,655,395 bp
  • A to G, chromosome 7 at 133,869,346 bp
  • A to G, chromosome 7 at 141,258,465 bp
  • C to A, chromosome 8 at 33,324,318 bp
  • A to T, chromosome 8 at 45,792,875 bp
  • A to C, chromosome 8 at 71,483,243 bp
  • A to T, chromosome 8 at 110,535,283 bp
  • T to C, chromosome 8 at 123,246,916 bp
  • A to G, chromosome 9 at 38,431,176 bp
  • T to C, chromosome 9 at 40,187,601 bp
  • T to C, chromosome 10 at 24,654,908 bp
  • T to A, chromosome 10 at 130,389,195 bp
  • G to T, chromosome 11 at 69,946,391 bp
  • T to C, chromosome 11 at 119,039,311 bp
  • T to A, chromosome 12 at 84,613,971 bp
  • T to A, chromosome 12 at 109,594,097 bp
  • A to G, chromosome 13 at 40,918,199 bp
  • A to G, chromosome 13 at 64,787,955 bp
  • C to A, chromosome 13 at 93,045,866 bp
  • T to C, chromosome 14 at 49,960,623 bp
  • A to T, chromosome 14 at 52,174,144 bp
  • T to C, chromosome 14 at 55,677,234 bp
  • A to G, chromosome 14 at 67,705,629 bp
  • A to G, chromosome 14 at 84,446,360 bp
  • G to A, chromosome 15 at 79,712,174 bp
  • C to T, chromosome 17 at 71,244,803 bp
  • T to G, chromosome 18 at 15,063,516 bp
  • A to G, chromosome 19 at 5,136,791 bp
  • A to G, chromosome 19 at 42,076,117 bp
  • A to T, chromosome Y at 6,916,501 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5695 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043326-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.