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Strain Name:
C57BL/6J-MtgxR5726Btlr/Mmmh
Stock Number:
043344-MU
Citation ID:
RRID:MMRRC_043344-MU
Other Names:
R5726 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Foxp4
Name: forkhead box P4
Synonyms: 1200010K03Rik, 2310007G05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74123
Homologene: 12536
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75725
Homologene: 8775
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Dgkh
Name: diacylglycerol kinase, eta
Synonyms: 5930402B05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 380921
VEGA: 14
HGNC: HGNC:2854
Homologene: 99373
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 162,808,259 bp
  • A to G, chromosome 1 at 177,448,553 bp
  • T to C, chromosome 2 at 69,509,147 bp
  • A to G, chromosome 2 at 90,717,851 bp
  • A to G, chromosome 2 at 118,443,132 bp
  • T to A, chromosome 2 at 120,584,271 bp
  • G to A, chromosome 2 at 121,305,065 bp
  • T to C, chromosome 2 at 148,396,083 bp
  • T to A, chromosome 3 at 5,403,321 bp
  • A to T, chromosome 3 at 90,129,207 bp
  • A to G, chromosome 4 at 66,840,415 bp
  • A to G, chromosome 4 at 117,864,013 bp
  • T to C, chromosome 4 at 129,071,892 bp
  • C to T, chromosome 5 at 92,943,005 bp
  • A to T, chromosome 5 at 109,217,620 bp
  • A to G, chromosome 5 at 115,479,646 bp
  • A to G, chromosome 5 at 150,991,538 bp
  • T to C, chromosome 6 at 11,933,538 bp
  • C to T, chromosome 6 at 86,925,124 bp
  • C to T, chromosome 6 at 87,463,459 bp
  • T to C, chromosome 6 at 88,848,739 bp
  • A to G, chromosome 7 at 44,643,462 bp
  • T to C, chromosome 7 at 75,124,214 bp
  • C to T, chromosome 7 at 85,559,107 bp
  • T to C, chromosome 7 at 126,575,501 bp
  • T to C, chromosome 7 at 128,236,660 bp
  • A to T, chromosome 9 at 56,885,904 bp
  • G to A, chromosome 9 at 67,023,412 bp
  • C to T, chromosome 9 at 119,533,847 bp
  • A to T, chromosome 10 at 12,669,806 bp
  • A to G, chromosome 10 at 41,483,696 bp
  • C to T, chromosome 10 at 60,407,480 bp
  • T to C, chromosome 10 at 83,512,306 bp
  • T to C, chromosome 10 at 107,123,322 bp
  • T to C, chromosome 10 at 116,956,887 bp
  • C to T, chromosome 10 at 121,267,574 bp
  • G to T, chromosome 11 at 6,509,420 bp
  • T to A, chromosome 11 at 67,294,566 bp
  • T to C, chromosome 11 at 69,633,346 bp
  • A to G, chromosome 11 at 113,851,800 bp
  • A to G, chromosome 11 at 119,416,458 bp
  • T to C, chromosome 12 at 36,547,830 bp
  • A to C, chromosome 12 at 70,078,179 bp
  • C to T, chromosome 12 at 70,191,142 bp
  • T to A, chromosome 12 at 86,162,183 bp
  • T to C, chromosome 12 at 114,896,674 bp
  • T to A, chromosome 13 at 23,459,352 bp
  • T to C, chromosome 13 at 76,118,347 bp
  • G to A, chromosome 14 at 45,165,764 bp
  • A to T, chromosome 14 at 55,783,661 bp
  • T to A, chromosome 14 at 75,330,829 bp
  • T to A, chromosome 14 at 76,505,317 bp
  • G to T, chromosome 14 at 78,624,902 bp
  • A to G, chromosome 15 at 91,260,503 bp
  • T to C, chromosome 16 at 20,710,535 bp
  • T to C, chromosome 16 at 38,208,136 bp
  • T to A, chromosome 16 at 49,699,498 bp
  • C to T, chromosome 17 at 20,913,339 bp
  • A to G, chromosome 17 at 47,869,108 bp
  • C to T, chromosome 17 at 86,120,729 bp
  • C to A, chromosome 18 at 12,697,935 bp
  • A to G, chromosome 18 at 34,331,773 bp
  • C to T, chromosome 18 at 36,670,173 bp
  • G to T, chromosome 18 at 57,896,354 bp
  • G to T, chromosome 19 at 6,351,332 bp
  • A to G, chromosome 19 at 12,241,280 bp
  • A to G, chromosome 19 at 18,853,617 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5726 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043344-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.