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Strain Name:
C57BL/6J-MtgxR5770Btlr/Mmmh
Stock Number:
043370-MU
Citation ID:
RRID:MMRRC_043370-MU
Other Names:
R5770 (G1)
Major Collection:

Strain Information

Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Phf21a
Name: PHD finger protein 21A
Synonyms: 80kDa, Braf35/HDAC complex (Bhc), PFTF1, Bhc80, D030065N23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192285
Homologene: 9597
1700030C10Rik
Name: RIKEN cDNA 1700030C10 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 69513
Kif11
Name: kinesin family member 11
Synonyms: Eg5, Knsl1, Kifl1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Zc3h4
Name: zinc finger CCCH-type containing 4
Synonyms: LOC330474, Bwq1, Kiaa1064-hp
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330474
Homologene: 87150
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Nudcd3
Name: NudC domain containing 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 209586
Homologene: 9097
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 178,256,495 bp
  • T to C, chromosome 2 at 26,414,390 bp
  • A to G, chromosome 2 at 31,017,508 bp
  • T to A, chromosome 2 at 89,816,549 bp
  • C to T, chromosome 2 at 92,351,854 bp
  • C to G, chromosome 2 at 132,872,156 bp
  • T to C, chromosome 2 at 141,232,182 bp
  • T to C, chromosome 2 at 158,195,580 bp
  • T to C, chromosome 3 at 97,215,175 bp
  • A to G, chromosome 4 at 34,806,747 bp
  • T to C, chromosome 4 at 59,092,466 bp
  • A to T, chromosome 4 at 96,077,432 bp
  • A to G, chromosome 6 at 40,669,804 bp
  • T to C, chromosome 6 at 85,817,675 bp
  • T to C, chromosome 6 at 146,555,073 bp
  • T to A, chromosome 7 at 10,715,487 bp
  • T to A, chromosome 7 at 16,429,611 bp
  • T to C, chromosome 7 at 27,212,988 bp
  • T to C, chromosome 7 at 28,299,009 bp
  • A to G, chromosome 7 at 81,078,562 bp
  • A to T, chromosome 7 at 105,090,895 bp
  • C to T, chromosome 7 at 122,179,907 bp
  • A to T, chromosome 7 at 128,248,547 bp
  • A to G, chromosome 7 at 140,610,943 bp
  • A to G, chromosome 8 at 71,457,328 bp
  • C to A, chromosome 8 at 117,055,018 bp
  • A to G, chromosome 8 at 123,235,720 bp
  • T to A, chromosome 9 at 37,419,201 bp
  • C to T, chromosome 9 at 40,006,338 bp
  • T to A, chromosome 9 at 42,345,589 bp
  • C to T, chromosome 9 at 71,645,487 bp
  • T to A, chromosome 9 at 76,259,666 bp
  • G to A, chromosome 10 at 6,789,026 bp
  • T to C, chromosome 10 at 62,286,449 bp
  • T to A, chromosome 10 at 74,185,345 bp
  • C to T, chromosome 10 at 75,638,019 bp
  • T to C, chromosome 11 at 6,113,286 bp
  • A to G, chromosome 11 at 49,514,592 bp
  • G to A, chromosome 11 at 58,786,594 bp
  • G to A, chromosome 11 at 67,297,200 bp
  • C to G, chromosome 11 at 72,760,256 bp
  • G to A, chromosome 11 at 99,772,555 bp
  • T to G, chromosome 11 at 101,331,752 bp
  • A to G, chromosome 11 at 115,472,778 bp
  • A to G, chromosome 12 at 11,092,249 bp
  • T to C, chromosome 12 at 20,815,459 bp
  • A to G, chromosome 12 at 85,087,380 bp
  • A to T, chromosome 12 at 100,878,821 bp
  • A to T, chromosome 13 at 38,937,758 bp
  • A to G, chromosome 13 at 47,014,712 bp
  • A to T, chromosome 13 at 74,323,120 bp
  • G to A, chromosome 14 at 93,886,943 bp
  • A to G, chromosome 14 at 113,315,375 bp
  • T to A, chromosome 15 at 69,049,463 bp
  • A to T, chromosome 16 at 59,329,151 bp
  • TCACACA to TCACA, chromosome 17 at 56,270,629 bp
  • A to T, chromosome 17 at 58,879,151 bp
  • A to G, chromosome 18 at 37,498,037 bp
  • A to G, chromosome 18 at 78,350,249 bp
  • T to C, chromosome 19 at 5,681,579 bp
  • A to T, chromosome 19 at 8,386,527 bp
  • G to T, chromosome 19 at 18,645,074 bp
  • A to G, chromosome 19 at 37,390,865 bp
  • T to A, chromosome 19 at 43,614,533 bp
  • T to A, chromosome 19 at 44,021,579 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5770 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043370-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.