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Strain Name:
C57BL/6J-MtgxR5781Btlr
Stock Number:
043378-MU
Citation ID:
RRID:MMRRC_043378-MU
Other Names:
R5781 (G1)
Major Collection:

Strain Information

Hyal1
Name: hyaluronoglucosaminidase 1
Synonyms: Hyal-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15586
HGNC: HGNC:5320
Homologene: 5277
Tgfb1
Name: transforming growth factor, beta 1
Synonyms: TGF-beta 1, Tgfb-1, Tgfb, TGFbeta1, TGF-beta1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21803
Homologene: 540
Msx2
Name: msh homeobox 2
Synonyms: Hox8.1, Hox8, Hox-8
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17702
HGNC: HGNC:7392
Homologene: 1837
Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78514
Homologene: 12695
Ubr5
Name: ubiquitin protein ligase E3 component n-recognin 5
Synonyms: 4432411E13Rik, Edd1, Edd
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70790
VEGA: 15
Homologene: 9295
Hspa12a
Name: heat shock protein 12A
Synonyms: Hspa12a, 1700063D12Rik, Gm19925
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73442
VEGA: 19
Homologene: 18422
Pnn
Name: pinin
Synonyms: D12Ertd512e
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18949
VEGA: 12
HGNC: HGNC:9162
Homologene: 37656
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 55,695,989 bp
  • A to G, chromosome 1 at 120,031,620 bp
  • T to C, chromosome 1 at 171,251,015 bp
  • A to G, chromosome 2 at 18,731,993 bp
  • G to T, chromosome 2 at 70,016,244 bp
  • C to A, chromosome 2 at 120,305,023 bp
  • T to C, chromosome 2 at 181,494,154 bp
  • A to C, chromosome 3 at 127,680,035 bp
  • C to T, chromosome 4 at 86,601,263 bp
  • C to T, chromosome 4 at 116,820,578 bp
  • G to A, chromosome 4 at 133,996,830 bp
  • T to C, chromosome 4 at 139,468,096 bp
  • A to T, chromosome 5 at 37,326,570 bp
  • A to C, chromosome 5 at 96,086,165 bp
  • T to A, chromosome 5 at 141,936,048 bp
  • A to T, chromosome 6 at 24,564,378 bp
  • G to A, chromosome 6 at 108,510,738 bp
  • G to T, chromosome 6 at 142,365,618 bp
  • A to G, chromosome 7 at 25,696,960 bp
  • T to A, chromosome 8 at 3,439,439 bp
  • G to T, chromosome 8 at 77,450,707 bp
  • G to A, chromosome 8 at 78,505,641 bp
  • G to A, chromosome 8 at 111,273,084 bp
  • A to G, chromosome 8 at 119,410,280 bp
  • A to T, chromosome 8 at 121,604,593 bp
  • A to T, chromosome 9 at 44,847,842 bp
  • C to T, chromosome 9 at 107,577,667 bp
  • A to G, chromosome 9 at 123,794,833 bp
  • T to A, chromosome 10 at 39,823,093 bp
  • T to A, chromosome 10 at 50,637,978 bp
  • T to A, chromosome 10 at 57,807,938 bp
  • T to G, chromosome 10 at 62,417,933 bp
  • T to A, chromosome 10 at 81,243,713 bp
  • A to T, chromosome 10 at 129,133,147 bp
  • A to G, chromosome 11 at 110,101,987 bp
  • T to C, chromosome 12 at 59,071,819 bp
  • C to A, chromosome 13 at 53,472,608 bp
  • T to G, chromosome 13 at 114,820,919 bp
  • G to T, chromosome 15 at 35,794,035 bp
  • T to C, chromosome 15 at 38,006,541 bp
  • A to G, chromosome 15 at 55,423,512 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • T to G, chromosome 17 at 57,524,938 bp
  • A to G, chromosome 18 at 20,032,510 bp
  • G to T, chromosome 18 at 58,837,968 bp
  • A to T, chromosome 18 at 68,407,395 bp
  • A to T, chromosome 18 at 68,407,397 bp
  • A to C, chromosome 19 at 38,882,683 bp
  • T to C, chromosome 19 at 58,822,086 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5781 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043378-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.