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Strain Name:
C57BL/6J-MtgxR5814Btlr/Mmmh
Stock Number:
043396-MU
Citation ID:
RRID:MMRRC_043396-MU
Other Names:
R5814 (G1)
Major Collection:

Strain Information

Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20349
Homologene: 8247
Vps33a
Name: VPS33A CORVET/HOPS core subunit
Synonyms: bf, 3830421M04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77573
Homologene: 11294
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Mmp10
Name: matrix metallopeptidase 10
Synonyms: stromelysin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17384
VEGA: 9
HGNC: HGNC:7156
Homologene: 20546
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Srsf11
Name: serine and arginine-rich splicing factor 11
Synonyms: 0610009J05Rik, 2610019N13Rik, Sfrs11
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69207
Homologene: 36164
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,199,405 bp
  • T to A, chromosome 1 at 131,271,779 bp
  • A to G, chromosome 1 at 136,107,142 bp
  • T to A, chromosome 1 at 180,669,198 bp
  • G to A, chromosome 1 at 181,055,098 bp
  • A to T, chromosome 2 at 153,672,497 bp
  • G to A, chromosome 3 at 86,126,604 bp
  • A to C, chromosome 3 at 102,895,897 bp
  • C to T, chromosome 3 at 158,023,344 bp
  • T to A, chromosome 4 at 115,432,497 bp
  • T to A, chromosome 4 at 156,047,627 bp
  • A to C, chromosome 5 at 8,973,996 bp
  • A to G, chromosome 5 at 14,225,666 bp
  • A to G, chromosome 5 at 16,602,307 bp
  • G to A, chromosome 5 at 105,119,919 bp
  • C to A, chromosome 5 at 110,695,578 bp
  • T to A, chromosome 5 at 115,979,419 bp
  • T to C, chromosome 5 at 123,565,056 bp
  • A to T, chromosome 6 at 48,451,884 bp
  • C to A, chromosome 6 at 100,733,235 bp
  • A to T, chromosome 6 at 136,911,566 bp
  • T to A, chromosome 7 at 8,471,476 bp
  • T to A, chromosome 7 at 13,340,618 bp
  • T to C, chromosome 7 at 42,107,098 bp
  • A to G, chromosome 7 at 92,657,714 bp
  • C to A, chromosome 7 at 105,719,895 bp
  • T to C, chromosome 7 at 131,139,082 bp
  • T to C, chromosome 8 at 3,923,348 bp
  • T to C, chromosome 8 at 60,934,573 bp
  • A to G, chromosome 8 at 105,843,971 bp
  • T to A, chromosome 8 at 106,912,275 bp
  • G to A, chromosome 9 at 7,503,620 bp
  • A to G, chromosome 9 at 45,860,264 bp
  • T to A, chromosome 9 at 110,567,758 bp
  • A to G, chromosome 9 at 120,128,485 bp
  • G to A, chromosome 9 at 120,424,668 bp
  • A to T, chromosome 10 at 18,200,009 bp
  • G to T, chromosome 10 at 33,528,507 bp
  • C to A, chromosome 10 at 77,716,123 bp
  • C to T, chromosome 10 at 81,085,662 bp
  • AGCGGTCGTAGGC to AGC, chromosome 11 at 73,395,654 bp
  • T to A, chromosome 11 at 94,082,828 bp
  • G to A, chromosome 11 at 103,138,383 bp
  • T to A, chromosome 11 at 104,736,114 bp
  • C to T, chromosome 11 at 115,984,157 bp
  • A to G, chromosome 13 at 47,063,146 bp
  • G to T, chromosome 13 at 80,890,579 bp
  • A to G, chromosome 14 at 31,124,520 bp
  • T to A, chromosome 15 at 102,483,162 bp
  • T to C, chromosome 16 at 88,924,114 bp
  • G to T, chromosome 16 at 91,030,496 bp
  • C to A, chromosome 19 at 8,774,846 bp
  • A to G, chromosome 19 at 17,016,361 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5814 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043396-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.