Strain Detail Sheet

Strain Name:
B6J(FVB)-Pitx1em1Chalu/Mmmh
Stock Number:
043534-MU
Citation ID:
RRID:MMRRC_043534-MU
Other Names:
PDE-/-,

Strain Information

Gene Details

Pitx1em1Chalu
Name: paired-like homeodomain transcription factor 1, endonuclease-mediated mutation 1, James Noonan
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Pitx1
Name: paired-like homeodomain transcription factor 1
Synonyms: Bft, Ptx1, P-OTX, Potx
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18740
HGNC: HGNC:9004
Homologene: 20584
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Genetic Alterations
A 13kb Pitx1 enhancer 132kb 3' of the promoter, shown to topologically interact with the target gene through 3C and 4C, was deleted via CRISPR. The deleted sequence spans chr13:56,055,928-56,068,947.
Genotype Determination
ES Cell Line
Not specified

Strain Description

Phenotype

Homozygous: The mice no longer topologically interact with the enhancer region. At E11.5 Pitx1 expression is reduced by 15% in the hindlimb and 39% in the mandibular arch. Their morphology is overtly normal and exhibit normal viability.

Hetero/Hemizygous: The heterozygous mice display a normal phenotype. In depth molecular analysis was not conducted on the hets.

Cre-excised Phenotype: Undetermined. 

Strain Development
A conditional allele was produced by using CRISPR to concurrently flox the 13kb enhancer sequence (the Pitx1 distal enhacner, or PDE) through pronuclear injection by the Yale Transgenic Facility. The founder mouse containing the two loxp sites was bred back to wt B6 mouse. This mouse was crossed with an actin cre expressing mouse to produce PDE-/- mice. These mice were then used in sib crosses to expand the line.
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Developmental Biology

Strain Origin

Donor
James Noonan, Ph.D., Yale University.

Colony and Husbandry Information

Special Considerations

None.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Backcross
Breeding Scheme(s)
Sib-mating
Generation
2
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.