Strain Name:
B6;129S4-Cdkn2atm2.1Nesh/Mmnc
Stock Number:
043540-UNC
Citation ID:
RRID:MMRRC_043540-UNC
Other Names:
p16INKa4a L , p16 Conditional, p16INK4a Conditional, p16-floxed, p16INK4a-floxed

Gene Information

Allele Symbol: Cdkn2atm2.1Nesh (Mus musculus (mouse))
Name: cyclin dependent kinase inhibitor 2A; targeted mutation 2.1, Norman E Sharpless
Synonyms: p16INKa4a L, p16
Chromosome: 4
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:4835008
Gene Symbol: Cdkn2a (Mus musculus (mouse))
Name: cyclin dependent kinase inhibitor 2A
Synonyms: INK4a-ARF, p16, Arf, Ink4a/Arf, p19, Pctr1, p16INK4a, p19ARF, MTS1, ARF-INK4a
Chromosome: 4
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:104738
Genetic Alterations
A Neo cassette comprised of a left loxP site, a FRT site, a PGK-neomycin resistance gene and another FRT site was inserted 5' to the exon 1a start site. An Nhe I site was introduced next to a StyI site. A non-conserved Variable Number Tandem Microsatellite repeat (VNTR) of approximately 560 bases was deleted immediately upstream of the StyI site. The right loxP site was introduced into the first intron. The neomycin cassette was excised by mating with Flp-recombinase expressing mice (obtained from Jackson Laboratories).
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    v6.5 derived from (C57BL/6 x 129S4/SvJae)F1
    Phenotype

    Homozygous: Same as wild-type until crossed with a Cre-expressing line.

    Hetero/Hemizygous: Same as wild-type until crossed with a Cre-expressing line.

    Cre-excised Phenotype: Undetermined.

    MeSH Terms
      Strain Development
      The p16INK4a conditional allele was created by inserting a neo cassette 3.5 Kb 5’ to the exon 1a start site. The Neo cassette comprised of a left loxP site, a FRT site, a PGK-neomycin resistance gene and another FRT site. An Nhe I site was introduced next to a StyI site. A non-conserved Variable Number Tandem Microsatellite repeat (VNTR) of approximately 560 bases was deleted immediately upstream of the StyI site. The right loxP site was introduced with engineered NotI and XhoI sites in an XbaI site in the first intron. Homologous targeting of the conditional allele was confirmed using southern blot for either targeting arm. The neomycin cassette was excised by mating with Flp-recombinase expressing mice (Jackson Laboratories).
      Suggested Control Mice
      Wild-type littermates
      • Cancer
      • Cell Biology
      • Dermatology
      • Hematology
      • Immunology and Inflammation
      • Models for Human Disease
      • Research Tools
      Donor
      Norman Sharpless, M.D., University of North Carolina at Chapel Hill.

      Colony and Husbandry Informationon

      Colony Surveillance Program and Current Health Reports

      Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
      Coat Color
      Black
      MMRRC Breeding System
      Sib-mating
      Breeding Scheme(s)
      Homozygous x homozygous
      Generation
      1 (C57BL/6)
      Overall Breeding Performance
      Good
      Viability and Fertility: Female Male Comments
      Homozygotes are viable: Yes Yes
      Homozygotes are fertile: Yes Yes
      Heterozygotes are fertile: Yes Yes
      Age Reproductive Decline: 6 to 8 months 8 to 12 months
      Average litter size
      5-9
      Recommended wean age
      3-4 weeks

      Order Request Information

      Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

      Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

      The donor or their institution limits the distribution to non-profit institutions only.

      Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

      Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
      MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
      043540-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
      043540-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity2
      043540-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

      1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

      2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

      3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

      To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.