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Availability & Fees Order this Strain
Homozygous: Same as wild type
Hetero/Hemizygous: Same as wild type
Cre-excised Phenotype: Undetermined
A BAC construct containing the mouse Pten was used as a genomic source to create a targeting vector. Briefly, a 5’ homology arm of exon 6 followed by exon 7 containing tyrosine 240 to phenylalanine mutation and a right homology arm containing exons 8 and 9 were inserted into targeting vector pKO scrambler V915 (Lexicon Genetics Inc., Woodland, TX) flanking a LoxP-pGK-Neo-LoxP selection cassette. The linearized targeting vector was electroporated into 129SvEvTac-dereived TC1 ES cells. Clones were screened by southern blot analysis for correct targeting into the Pten locus. Two correctly targeted ES cell clones were injected into blastocysts to generate chimeric mice in C57BL/6J. Mice with greater than 80% chimerism, as determined by agouti coat color, were bred with C57BL/6 mice and progeny were screened by southern blot to ascertain germline transmission. Heterozygous mice were bred with E2a-cre mice (Jackson Lab, #003314) to excise the pGK-Neo selection cassette and progeny were screened by PCR to confirm successful cre-mediated excision. The strian donor reports that the mice are maintained on a C57BL/6NCrl background.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.